Molecular cloning, expression and purification of protein 2A of hepatitis A virus

New Microbiologica

Volumn 27, Issue 2, 2004, Pages 105-112

  Di Napoli, A ^a   Maltese, E ^b   Bucci, M ^b   Pagnotti, P ^b   Seipelt, J ^c   Duquerroy, S ^a   Perez Bercoff R ^a,b  

^a Lab Virologie Molec S   (France)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c UNIVERSITY OF VIENNA   (Austria)

Author keywords

Expression; Hepatitis A virus; His tag; Molecular cloning; Protein 2A

Indexed keywords

AMINO ACID DERIVATIVE; AMMONIUM SULFATE; CELL EXTRACT; COMPLEMENTARY DNA; DNA FRAGMENT; IMIDAZOLE; INITIATION FACTOR 4G; NICKEL; POLYPEPTIDE; PRIMER DNA; PROTEIN 2A; PROTEIN DERIVATIVE; SEPHAROSE; UNCLASSIFIED DRUG; VIRUS PROTEIN; CYSTEINE PROTEINASE; HISTIDINE; PICORNAIN 2A, PICORNAVIRUS;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AFFINITY CHROMATOGRAPHY; ARTICLE; BIOAVAILABILITY; CHEMICAL BOND; CONTROLLED STUDY; CRYSTALLIZATION; DEGRADATION; DERIVATIZATION; DNA DENATURATION; DNA SEQUENCE; ENERGY YIELD; ESCHERICHIA COLI; EXPRESSION VECTOR; EXTRACTION; FRAGMENTATION REACTION; GENE CONSTRUCT; GENE EXPRESSION; GENE IDENTIFICATION; GENE SEGREGATION; GENETIC CODE; GENETIC VARIABILITY; HELA CELL; HEPATITIS A VIRUS; INCUBATION TIME; LIGHT SCATTERING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROBIOLOGY; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRECIPITATION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; SOLUBILIZATION; TRANSLATION INITIATION; WESTERN BLOTTING; AMINO ACID SEQUENCE; CHEMISTRY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MOLECULAR GENETICS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; HEPATITIS A VIRUS; VECTORS;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; CRYSTALLIZATION; CYSTEINE ENDOPEPTIDASES; GENE EXPRESSION REGULATION, VIRAL; HEPATITIS A VIRUS; HISTIDINE; MOLECULAR SEQUENCE DATA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; VIRAL PROTEINS;

EID: 3042622670     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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