Automated Process of Q-PCR/Gene Expression

Journal of Laboratory Automation

Volumn 9, Issue 3, 2004, Pages 128-134

  Cherry, James M ^a   Stewart, Claudia ^a   Frankenberger, Casey ^a   Martin, Kelly ^a   Tudor, Gabriela ^a   Owens, Garrison ^a   Bhat, Narayan K ^a   Munroe, David J ^a  

^a SAIC FREDERICK INC   (United States)

Author keywords

automation; high throughput; quantitative PCR

Indexed keywords

COMPLEMENTARY DNA; RNA;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; AUTOMATION; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DEVICE; DIAGNOSTIC VALUE; DNA SYNTHESIS; FLUORESCENCE ANALYSIS; GENE EXPRESSION; LABORATORY AUTOMATION; NONHUMAN; POLYMERASE CHAIN REACTION; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; ROBOTICS;

EID: 3042599891     PISSN: 22110682     EISSN: 15402452     Source Type: Journal    
DOI: 10.1016/j.jala.2004.04.011     Document Type: Article

References (7)

1. Detlef B. Sabine H. Alma K. Thomas L. Fanco L. Rapid detection of subtelomeric deletion duplication by novel real-time quantitative PCR using SYBR Green dye. Human Mutation 2004, 23, 368–378.
2. Leb V. Stocher M. Valentine-Thon E. Holzl G. Kessler H. Stekel H. Berg J. Fully automated, internally controlled quantification of hepatitis B Virus DNA by real-time PCR by use of the MagNA Pure LC and LightCycler instruments. J. Microbiol. 2004, 42, 585–590.
3. Richard M. L. Frappier R. H. Newman J. C. Developmental validation of a real-time quantitative PCR assay for automated quantification of human DNA. J. Forensic Sci. 2003, 48, 1041–1046.
4. Emery S. L. Real-time reverse transcription-polymerase chain reaction assay for SARS-associated Coronavirus. Emerg. Infectious Disease 2004, 2, 311–316.
5. Khandurina J. Chang H.-S. Wanders B. Automated high throughput RNA analysis by capillary electrophoresis. Bio Tech. 2002, 32, 1226–1230.
6. Stewart C. Lum N. Hill R. Stephens R. Rudnick P. Rasmussen L. Munroe D. High throughput primer walking of cDNA clones. J. Assoc. Lab. Auto. 2003, 8, 64–65.
7. Lee B.-I. Oak S. Khietala S. Schikora B. Hecker K. H. Automated, high-throughput total and viral RNA purification 15th Annual Genome Sequencing and Analysis Conference (GSAC XV) 2003, 15, 159.