Real-time quantitative RT-PCR method for estimation of mRNA level of CCAAT/enhancer binding protein in the central nervous system of Lymnaea stagnalis

Acta Biologica Hungarica

Volumn 55, Issue 1-4, 2004, Pages 157-161

  Hatakeyama, D ^a,b   Sadamoto, Hisayo ^a   Ito, E ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b TOKAI UNIVERSITY   (Japan)

Author keywords

C EBP; CNS; Lymnaea; mRNA; qRT PCR

Indexed keywords

CCAAT ENHANCER BINDING PROTEIN; COMPLEMENTARY DNA; MESSENGER RNA; RNA;

ALTERNATIVE RNA SPLICING; ANIMAL; BIOLOGICAL MODEL; BIOSYNTHESIS; CENTRAL NERVOUS SYSTEM; COMPUTER PROGRAM; CONFERENCE PAPER; LYMNAEA; METABOLISM; METHODOLOGY; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ALTERNATIVE SPLICING; ANIMALS; CCAAT-ENHANCER-BINDING PROTEINS; CENTRAL NERVOUS SYSTEM; DNA, COMPLEMENTARY; LYMNAEA; MODELS, GENETIC; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; SOFTWARE;

GASTROPODA; INVERTEBRATA; LYMNAEA; LYMNAEA STAGNALIS; LYMNAEIDAE;

EID: 3042584662     PISSN: 02365383     EISSN: None     Source Type: Journal    
DOI: 10.1556/ABiol.55.2004.1-4.19     Document Type: Conference Paper

References (7)

1. Bustin, S. A. (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J. Mol. Endocrinol. 29, 23-29.
2. Hatakeyama, D., Ito, E. (1999) Three-dimensional reconstruction and mapping of serotonin-like immunoreactive neurons in the central nervous system of the pond snail, Lymnaea stagnalis, with the confocal laser scanning microscope. Bioimages 7, 1-12.
3. Hatakeyama, D., Ito, E. (2000) Distribution and developmental changes in GABA-like immunoreactive neurons in the central nervous system of pond snail, Lymnaea stagnalis. J. Comp. Neurol. 418, 310-322.
4. Hatakeyama, D., Ito, I., Fujito, Y., Ito, E. (2000) Complement receptor 3-like immunoreactivity in the light green cells and the canopy cells of the pond snail, Lymnaea stagnalis. Brain Res. 865, 102-106.
5. Kojima, S., Nakamura, H., Nagayama, S., Fujito, Y., Ito, E. (1997) Enhancement of an inhibitory input to the feeding central pattern generator in Lymnaea stagnalis during conditioned taste-aversion learning. Neurosci. Lett. 230, 179-182.
6. Shi, L., Ho, J., Norling, L. A., Roy, M., Xu, Y., (1999) A real time quantitative PCR-based method for the detection and quantification of simian virus 40. Biologicals 27, 241-252.
7. Wall, S. J., Edwards, D. R. (2002) Quantitative reverse transcription-polymerase chain reaction (RT-PCR): A comparison of primer-dropping, competitive, and real-time RT-PCRs. Anal. Biochem. 15, 269-273.