Manual exfoliation of fresh tissue obviates the need for frozen sections for molecular profiling

Cancer

Volumn 105, Issue 6, 2005, Pages 483-491

  Mojica, Wilfrido D ^a   Rapkiewicz, Amy V ^b   Liotta, Lance A ^c   Espina, Virginia ^c  

^a UNIVERSITY AT BUFFALO   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c GEORGE MASON UNIVERSITY   (United States)

Author keywords

Cryosection; Genomics; Laser capture microdissection; Molecular profiling; Nucleic acid; Protein; Proteomics; Translational research

Indexed keywords

CELL PROTEIN; NUCLEIC ACID;

ARTICLE; CELL POPULATION; CYTOLOGY; FROZEN SECTION; GENOMICS; HUMAN; HUMAN TISSUE; LASER CAPTURE MICRODISSECTION; MACROMOLECULE; MANUAL EXFOLIATION; MOLECULAR BIOLOGY; MOLECULAR PROFILING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN MICROARRAY; PROTEOMICS; RESEARCH; REVERSE PHASE PROTEIN MICROARRAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SAMPLING; SMEAR; SOLID TUMOR; TECHNIQUE; TISSUE PRESERVATION; TRANSLATIONAL RESEARCH;

CELL SEPARATION; DNA; FROZEN SECTIONS; HUMANS; LASERS; MICRODISSECTION; POLYMERASE CHAIN REACTION; PRESERVATION, BIOLOGICAL; PROTEOME; RNA; SPECIMEN HANDLING; TISSUE ARRAY ANALYSIS; TISSUE FIXATION;

EID: 30344447275     PISSN: 0008543X     EISSN: None     Source Type: Journal    
DOI: 10.1002/cncr.21347     Document Type: Article

References (21)

1. Yang GC, Hoda SA. Combined use of the "scratch and smear" sampling technique and Ultrafast Papanicolaou stain for intraoperative cytology. Acta Cytol. 1997;41:1513-1518.
2. Stanley MW, Lowhagen T. Fine needle aspiration of palpable masses. Boston: Butterworth-Heinemann, 1993.
3. DeMay RM. The gastrointestinal tract. In: Rogers P, editor. The art and science of cytopathology. Chicago: ASCP Press, 1995:353-356.
4. Roemen G, Lentjes M, Luchtenborg M, et al. Reliable mutation analysis of archival tissue: critical issues and procedures [abstract 6072]. Proc Amer Assoc Cancer Res. 2003;44:1213-1214.
5. Wand G, Brennan C, Rook M, et al. Balanced PCR amplification allows unbiased identification of genomic copy changes in minute cell and tissue samples. Nucleic Acids Res. 2004;32:e76. Available from URL: www.nar.oupjournals.org/ [accessed November 20, 2004].
6. Paweletz CP, Charboneau L, Bichsel VE, et al. Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene. 2001;20:1981-1989.
7. Mohsin SK, Allred DC. Immunohistochemical (IHC) expression of phosphorylated proteins (PP) in breast cancer is significantly affected by formalin fixation [abstract 166]. Mod Pathol. 2004;17(S1):43A.
8. Sharifi AM, Roach C, Wakamiya K, Askaa J. Deterioration of phosphor-epitopes prior to and during specimen fixation [abstract 778]. Proc Amer Assoc Cancer Res. 2005;46:189.
9. Emmert-Buck MR, Stausberg RL, Krizman DB, et al. Molecular profiling of clinical tissue specimens. Feasibility and applications. J Mol Diagn. 2000;2:60-66.
10. Srinivasan M, Sedmak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol. 2002;161:1961-1971.
11. Fetsch PA, Simone NL, Bryant-Greenwood PK, et al. Proteomic evaluation of archival cytologic material using SELDI affinity mass spectrometry. Potential for diagnostic applications. Am J Clin Pathol. 2002;118:870-876.
12. Frayling IM. Methods of molecular analysis: mutation detection in solid tumors. Mol Pathol. 2002;55:73-79.
13. Bielawski K, Zaczek A, Lisowska U, Dybikowska A, Kowalska A, Falkiewicz B. The suitability of DNA extracted from formalin-fixed, paraffin-embedded tissues for double differential polymerase chain reaction analysis. Int J Mol Med. 2001;8:573-578.
14. Specht K, Richter T, Muller U, Walch A, Werner M, Hofler H. Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue. Am J Pathol. 2001;158:419-429.
15. Simpson RJ, Dorow DS. Cancer proteomics: from signaling networks to tumor markers. Trends Biotechnol. 2001;19:S40-S48.
16. Emmert-Buck MR, Bonner RF, Smith PD, et al. Laser capture microdissection. Science. 1996;274:996-1001.
17. Nagel H, Schulten HJ, Gunawan B, Brinck U, Fozesi L. The potential value of comparative genomic hybridization analysis in effusion and fine needle aspiration cytology. Mod Pathol. 2002;15:818-825.
18. Liu Y, Silverman JF, Sturgis CD, Brown HG, Dabbs DJ, Raab SS. Utility of intraoperative consultation touch preparations. Diagn Cytopathol. 2002;26:329-333.
19. Serth J, Kuczyk MA, Paeslack U, Lichtinghagen R, Jonas U. Quantitation of DNA extracted after micropreparation of cells from frozen and formalin-fixed tissue sections. Am J Pathol. 2000;156:1189-1196.
20. Abrahamsen HN, Steiniche T, Nexo E, Hamilton-Dutoit SJ, Sorenson BS. Towards quantitative mRNA analysis in paraffin embedded tissues using real-time reverse transcriptase-polymerase chain reaction. J Mol Diagn. 2003;5:34-41.
21. Farrand K, Jovanovic L, Delahunt B, et al. Loss of heterozygosity studies revisited. Prior quantification of the amplifiable DNA content of archival samples improves efficiency and reliability. J Mol Diagn. 2002;4:150-158.