26 kDa endochitinase from barley seeds: An interaction of the ionizable side chains essential for catalysis

Journal of Biochemistry

Volumn 138, Issue 5, 2005, Pages 553-562

  Ohnishi, Tsuneo ^a   Juffer, André H ^b   Tamoi, Masahiro ^a   Skriver, Karen ^c   Fukamizo, Tamo ^a  

^a KINKI UNIVERSITY   (Japan)

^b UNIVERSITY OF OULU   (Finland)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Catalytic cleft; Chitinase; Site directed mutagenesis; Theoretical pK a calculation; Thermal unfolding

Indexed keywords

ARGININE; CHITINASE; ENDOCHITINASE; GLUTAMIC ACID; GLYCOSIDASE; GUANIDINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BARLEY; CALCULATION; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENETIC CONSERVATION; IONIZATION; MOLECULAR INTERACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PKA; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; STREPTOMYCES; SURFACE CHARGE; THERMOSTABILITY; TRANSITION TEMPERATURE;

AMINO ACID SEQUENCE; CATALYSIS; CATALYTIC DOMAIN; CHITINASE; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, PLANT; HORDEUM; HYDROGEN-ION CONCENTRATION; IONS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI; HORDEUM VULGARE SUBSP. VULGARE; STREPTOMYCES; STREPTOMYCES SP.;

EID: 30344437875     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvi154     Document Type: Article

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