MALDI-TOF mass spectrometry of a combinatorial peptide library: Effect of matrix composition on signal suppression

Journal of Mass Spectrometry

Volumn 40, Issue 12, 2005, Pages 1590-1594

  Schlosser, Gitta ^a   Pocsfalvi, Gabriella ^b   Huszár, Emoke ^a   Malorni, Antonio ^b   Hudecz, Ferenc ^a,c  

^a EÖTVÖS LORÁND UNIVERSITY   (Hungary)

^b CNR   (Italy)

^c EÖTVÖS LORÁND UNIVERSITY   (Hungary)

Author keywords

Combinatorial chemistry; MALDI; Mass spectrometry; Peptide library; Signal suppression

Indexed keywords

COMPOSITION; INTERFERENCE SUPPRESSION; MASS SPECTROMETRY; POSITIVE IONS; PROTEINS;

COMBINATORIAL CHEMISTRY; MALDI; PEPTIDE LIBRARY; SIGNAL SUPPRESSION;

AMINO ACIDS;

AMINO ACID; ARGININE; PENTAPEPTIDE; PEPTIDE LIBRARY;

AMINO ACID SEQUENCE; ANALYTIC METHOD; CHEMICAL COMPOSITION; COMBINATORIAL CHEMISTRY; COMBINATORIAL LIBRARY; CONFERENCE PAPER; HIGH THROUGHPUT SCREENING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MODEL; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; TECHNIQUE;

COMBINATORIAL CHEMISTRY TECHNIQUES; OLIGOPEPTIDES; PEPTIDE LIBRARY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 30044444551     PISSN: 10765174     EISSN: None     Source Type: Journal    
DOI: 10.1002/jms.937     Document Type: Conference Paper

References (15)

1. Furka A, Sebestyén F, Asgedom M, Dibó G. General method for rapid synthesis of multicomponent peptide mixtures. Int. J. Pept. Protein Res. 1991; 37: 487.
2. Furka A, Bennett WD. Combinatorial libraries by portioning and mixing. Comb. Chem. High Throughput Screen. 1999; 2: 105.
3. Jung G (ed). Combinatorial Chemistry. Synthesis, Analysis, Screening. Wiley-VHC: Weinheim, 1999.
4. Jung G (ed). Combinatorial Peptide and Nonpeptide Libraries. Wiley-VHC: Weinheim, 1996.
5. Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. Electrospray ionization for mass-spectrometry of large biomolecules. Science 1989; 246: 64.
6. Demirev PA, Zubarev RA. Probing combinatorial library diversity by mass spectrometry. Anal. Chem. 1997; 69: 2893.
7. Windberg E, Hudecz F, Marquardt A, Sebestyén F, Kiss A, Bosze S, Medzihradszky-Schweiger H, Przybylski M. Characterization of combinatorial libraries of mucin-2 antigen peptides by high-resolution mass spectrometry. Rapid Commun. Mass Spectrom. 2002; 16: 834.
8. Schlosser G, Takáts Z, Vékey K, Pocsfalvi G, Malorni A, Windberg E, Kiss A, Hudecz F. Mass spectrometric analysis of combinatorial peptide libraries derived from the tandem repeat unit of MUC2 Mucin. J. Pept. Sci. 2003; 9: 361.
9. Uray K, Price MR, Majer Z, Vass E, Hollósi M, Hudecz F. Identification and solution conformation of multiple epitopes recognized by a MUC2 mucin specific monoclonal antibody. Arch. Biochem. Biophys. 2003; 410: 254.
10. 2T peptide mixtures by mucin-2 protein specific monoclonal antibody. J. Pept. Sci. 2004; 10: 56.
11. Smirnov IP, Zhu X, Taylor T, Huang Y, Ross P, Papayanopoulos IA, Martin SA, Pappin DJ. Suppression of α-cyano-4-hydroxycinnamic acid matrix clusters and reduction of chemical noise in MALDI-TOF mass spectrometry. Anal. Chem. 2004; 76: 2958.
12. Nordhoff E, Kirpenar F, Roepstorff P. Mass spectrometry of nucleic acids. Mass Spectrom. Rev. 1996; 15: 67.
13. Tang X, Callahan JH, Zhou P, Vertes A. Noncovalent protein- oligonucleotide interactions monitored by matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem. 1995; 67: 4542.
14. Schlosser G, Pocsfalvi G, Malorni A, Puerta A, de Frutos M, Vékey K. Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2003; 17: 2741.
15. Laugesen S, Roepstorff P. Combination of two matrices results in improved performance of MALDI MS for peptide mass mapping and protein analysis. J. Am. Soc. Mass Spectrom. 2003; 14: 992.