Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor

BMC Biotechnology

Volumn 5, Issue , 2005, Pages

  Saini, Deepti ^a,b   Kala, Mrinalini ^a,c   Jain, Vishal ^a   Sinha, Subrata ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

^b UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; ANTIGENS; BINDING ENERGY; BIOCHEMISTRY; CLONING; CONFORMATIONS; ENZYME INHIBITION; IMMUNOLOGY; MOLECULES; PHOSPHATES; SUBSTRATES;

ACTIVE SITE; ALKALINE PHOSPHATASE; ISOZYME SPECIFICITY; PARA-NITRO PHENYL PHOSPHATE; PLACENTAL ISOZYME; UNCOMPETITIVE INHIBITOR;

ENZYMES;

4 NITROPHENYL PHOSPHATE; ALKALINE PHOSPHATASE PLACENTA ISOENZYME; ENZYME ANTIBODY; ENZYME INHIBITOR; GLYCYLGLYCINE; PHENYLALANYLGLYCINE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; ALKALINE PHOSPHATASE; IMMUNOGLOBULIN FRAGMENT; ISOENZYME; MONOCLONAL ANTIBODY; PEPTIDE;

ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; COMBINATORIAL LIBRARY; COMPETITIVE INHIBITION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME CONFORMATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; INCUBATION TIME; MOLECULAR CLONING; MOLECULAR PROBE; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PURIFICATION; BINDING SITE; DRUG ANTAGONISM; DRUG DELIVERY SYSTEM; ENZYMOLOGY; GENE TARGETING; IMMUNOLOGY; METHODOLOGY; PEPTIDE LIBRARY; PLACENTA; PROTEIN ANALYSIS; PROTEIN BINDING;

ALKALINE PHOSPHATASE; ANTIBODIES, MONOCLONAL; BINDING SITES; DRUG DELIVERY SYSTEMS; ENZYME INHIBITORS; GENE TARGETING; IMMUNOGLOBULIN FRAGMENTS; ISOENZYMES; PEPTIDE LIBRARY; PEPTIDES; PLACENTA; PROTEIN BINDING; PROTEIN INTERACTION MAPPING;

EID: 30044432079     PISSN: 14726750     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-5-33     Document Type: Article

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