Partial dehydration of the retinal binding pocket and proof for photochemical deprotonation of the retinal Schiff base in bicelle bacteriorhodopsin crystals

Photochemistry and Photobiology

Volumn 81, Issue 6, 2005, Pages 1356-1360

  Sanii, Laurie S ^a   El Sayed, Mostafa A ^a  

^a GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIORHODOPSIN; PROTON; RETINAL; SCHIFF BASE;

ARTICLE; BINDING SITE; CELL LINE; CHEMISTRY; CRYSTALLIZATION; DESICCATION; HALOBACTERIUM SALINARUM; HEAT; LIGHT; METABOLISM; PHOTOCHEMISTRY; RADIATION EXPOSURE; RAMAN SPECTROMETRY;

BACTERIORHODOPSINS; BINDING SITES; CELL LINE; CRYSTALLIZATION; DESICCATION; HALOBACTERIUM SALINARUM; HEAT; LIGHT; PHOTOCHEMISTRY; PROTONS; RETINALDEHYDE; SCHIFF BASES; SPECTRUM ANALYSIS, RAMAN;

EID: 29844433028     PISSN: 00318655     EISSN: None     Source Type: Journal    
DOI: 10.1562/2005-03-09-RA-458     Document Type: Article

References (36)

1. Goldschmidt, C. R., M. Ottolenghi and R. Korenstein (1976) On the primary quantum yields in the bacteriorhodopsin photocycle. Biophys. J. 16, 839-843.
2. Fodor, S. P., J. B. Ames, R. Gebhard, E. M. van den Berg, W. Stoeckenius, J. Lugtenburg and R. A. Mathies (1988) Chromophore structure in bacteriorhodopsin's N intermediate: implications for the proton-pumping mechanism. Biochemistry 27, 7097-7101.
3. Oesterhelt, D. and B. Hess (1973) Reversible photolysis of the purple complex in the purple membrane of Halobacterium halobium. Eur. J. Biochem. 37, 316-326.
4. Stoeckenius, W. and R. H. Lozier (1974) Light energy conversion in Halobacterium halobium. J. Supramol. Struct. 2, 769-774.
5. Lozier, R. H., R. A. Bogomolni and W. Stoeckenius (1975) Bacteriorhodopsin, a light-driven proton pump in Halobacterium halobium. Biophys. J. 15, 955-962.
6. Jan, L. Y. (1975) Isomeric configuration of the bacteriorhodopsin chromophore. Vision Res. 15, 1081-1086.
7. Henderson, R. (1977) The purple membrane from Halobacterium halobium. Annu. Rev. Biophys. Bioeng. 6, 87-109.
8. Stoeckenius, W., R. H. Lozier and R. A. Bogomolni (1979) Bacteriorhodopsin and the purple membrane of halobacteria. Biochim. Biophys. Acta 505, 215-278.
9. Michel, H. and D. Oesterhelt (1976) Light-induced changes of the pH gradient and the membrane potential in H. halobium. FEBS Lett. 65, 175-178.
10. Bakker, E. P., H. Rottenberg and S. R. Caplan (1976) An estimation of the light-induced electrochemical potential difference of protons across the membrane of Halobacterium halobium. Biochim. Biophys. Acta 440, 557-572.
11. Belyakova, T. N., J. Kadziauskas, V. P. Skulachev, I. A. Smirnova, L. N. Chekulaeva and A. Jasaitis (1975) Generation of hydrogen(1+) ion electrochemical potential and photophosphorylation in Halobacterium halobium cells. Dokl. Akad. Nauk SSSR 223, 483-486.
12. Lewis, A., J. Spoonhower, R. A. Bogomolni, R. H. Lozier and W. Stoeckenius (1974) Tunable laser resonance Raman spectroscopy of bacteriorhodopsin. Proc. Natl. Acad. Sci. USA 71, 4462-4466.
13. Braiman, M. and R. Mathies (1982) Resonance Raman spectra of bacteriorhodopsin's primary photoproduct: evidence for a distorted 13-cis retinal chromophore. Proc. Natl. Acad. Sci. USA 79, 403-407.
14. Mathies, R. A., C. H. Brito Cruz, W. T. Pollard and C. V. Shank (1988) Direct observation of the femtosecond excited-state cis-trans isomerization in bacteriorhodopsin. Science 240, 777-779.
15. Pollard, W. T., C. H. Cruz, C. V. Shank and R. A. Mathies (1989) Direct observation of the excited-state cis-trans photoisomerization of bacteriorhodopsin: multilevel line shape theory for femtosecond dynamic hole burning and its application. J. Chem. Phys. 90, 199-208.
16. Ames, J. B. and R. A. Mathies (1990) The role of back-reactions and proton uptake during the N-O transition in bacteriorhodopsin's photocycle: a kinetic resonance Raman study. Biochemistry 29, 7181-7190.
17. Braiman, M. S., T. Mogi, L. J. Stern, N. R. Hackett, B. H. Chao, H. G. Khorana and K. J. Rothschild (1988) Vibrational spectroscopy of bacteriorhodopsin mutants. I. Tyrosine-185 protonates and deprotonates during the photocycle. Proteins 3, 219-229.
18. Gerwert, K., B. Hess, J. Soppa and D. Oesterhelt (1989) Role of aspartate-96 in proton translocation by bacteriorhodopsin. Proc. Natl. Acad. Sci. USA 86, 4943-4947.
19. Varo, G. and J. K. Lanyi (1991) Thermodynamics and energy coupling in the bacteriorhodopsin photocycle. Biochemistry 30, 5016-5022.
20. Bowie, J. and S. Farham (2002) Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure. J. Mol. Biol. 316, 1-6.
21. Landau, E. M. and J. P. Rosenbusch (1996) Lipidic cubic phases: a novel concept for the crystallization of membrane proteins. Proc. Natl. Acad. Sci. USA 93, 14532-14535.
22. Sanii, L. S., A. W. Schill, C. E. Moran and M. A. El-Sayed (2005) The protonation-deprotonation kinetics of the protonated Schiff base in bicelle bacteriorhodopsin crystals. Biophys. J. 89, 444-451.
23. Alshuth, T., I. Grieger, and M. Stockburger and Max-Planck-Institute of Biophysical Chemistry, Federal Republic of Germany (1982) Time-resolved resonance Raman studies of the photochemical cycle of bacteriorhodopsin. In Time-Resolved Vibrational Spectroscopy (Edited by G. H. Atkinson), pp. 231-237. Academic Press, New York, Proceedings of the International Conference on Time-Resolved Vibrational Spectroscopy, 1982.
24. Schenkl, S., E. Portuondo, C. Zgrablic, M. Chergui, W. Suske, M. Dolder, E. M. Landau and S. Haacke (2003) Compositional heterogeneity reflects partial dehydration in three-dimensional crystals of bacteriorhodopsin. J. Mol. Biol. 329, 711-719.
25. Salem, F., D. Yang, E. Bare, S. Yohannan, J. P. Whitelegge and J. U. Bowie (2004) Side-chain contributions to membrane protein structure and stability. J. Mol. Biol. 335, 297-305.
26. Lin, S. W., S. P. A. Fodor, L. J. W. Miercke, R. F. Shand, M. C. Betlach, R. M. Stroud and R. A. Mathies (1991) Resonance Raman spectra of bacteriorhodopsin mutants with substitutions at Asp-85, Asp-96, and Arg-82. Photochem. Photobiol. 53, 341-346.
27. Mathies, R., T. B. Freedman and L. Stryer (1977) Resonance Raman studies of the conformation of retinal in rhodopsin and isorhodopsin. J. Mol. Biol. 109, 367-372.
28. Oseroff, A. R. and R. H. Callender (1974) Resonance Raman spectroscopy of rhodopsin in retinal disk membranes. Biochemistry 13, 4243-4248.
29. Hildebrandt, P. and M. Stockburger (1984) Role of water in bacteriorhodopsin's chromophore: resonance Raman study. Biochemistry 23, 5539-5548.
30. Stockburger, M., W. Klusmann, H. Gattermann, G. Massig and R. Peters (1979) Photochemical cycle of bacteriorhodopsin studied by resonance Raman spectroscopy. Biochemistry 18, 4886-4900.
31. Braiman, M. and R. Mathies (1980) Resonance Raman evidence for an all-trans to 13-cis isomerization in the proton-pumping cycle of bacteriorhodopsin. Biochemistry 19, 5421-5428.
32. Henderson, R., M. Sulkes, A. Lewis and M. A. Marcus (1978) Resonance Raman spectroscopy of squid and bovine visual pigments: the primary photochemistry in visual transduction. Biochemistry 17, 4712-4722.
33. Althaus, T., W. Eisfeld, R. Lohrmann and M. Stockburger (1995) Application of Raman spectroscopy to retinal proteins. Isr. J. Chem. 35, 227-251.
34. Chronister, E. L. and M. A. El-Sayed (1987) Time-resolved resonance Raman spectra of the photocycle intermediates of acid and deionized bacteriorhodopsin. Photochem. Photobiol. 45, 507-513.
35. Aton, B., A. G. Doukas, R. H. Callender, B. Becher and T. G. Ebrey (1977) Resonance Raman studies of the purple membrane. Biochemistry 16, 2995-2999.
36. Netto, M. M., S. P. A. Fodor and R. A. Mathies (1990) Ultraviolet resonance Raman spectroscopy of bacteriorhodopsin. Photochem. Photobiol. 52, 605-607.