Modification of the standard trizol-based technique improves the integrity of RNA isolated from RNase-rich placental tissue [8]

Clinical Chemistry

Volumn 52, Issue 1, 2006, Pages 159-160

  Haimov Kochman, Ronit ^a   Fisher, Susan J ^a,b   Winn, Virginia D ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBONUCLEASE; RNA;

CAPILLARY ELECTROPHORESIS; ENZYME ACTIVITY; GENE EXPRESSION; LETTER; PLACENTA; RNA DEGRADATION; RNA EXTRACTION; RNA ISOLATION;

ELECTROPHORESIS, CAPILLARY; FEMALE; GUANIDINES; HUMANS; INDICATORS AND REAGENTS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHENOLS; PLACENTA; PREGNANCY; RIBONUCLEASES; RNA;

EID: 29744470585     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2005.059758     Document Type: Letter

References (6)

1. Berger SL, Cooper HL. Very short-lived and stable mRNAs from resting human lymphocytes. Proc Natl Acad Sci U S A 1975;72:3873-7.
2. Auer H, Lyianarachchi S, Newsom D, Klisovic MI, Marcucci G, Kornacker K, et al. Chipping away at the chip bias: RNA degradation in microarray analysis. Nat Genet 2003;35:292-3.
3. Chirgwin JM, Praybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 1979;18:5294-9.
4. Krosting J, Latham G. RNase activity in mouse tissue: classification, hierarchy, and methods for control. Ambion TechNotes, Vol. 12, issue 3. Austin, TX: Ambion, 2005.
5. Van de Goor TA. The principle and promise of labchip technology. Pharma Genomics 2003;3: 16-8.
6. Spruessel A, Steimann G, Jung M, Lee SA, Cart T, Fentz AK, et al. Tissue ischemia time affects gene and protein expression patterns within minutes following surgical tumor excision. Biotechniques 2004;36:1030-7.