An improved zymographic method for detection of amylolytic enzymes of fungi on polyacrylamide gels

Mycologist

Volumn 19, Issue 4, 2005, Pages 138-140

  Upadhyay, Mukesh K ^a   Sharma, Rahul ^a   Pandey, Akhilesh K ^a   Rajak, Ram C ^a  

^a RANI DURGAVATI UNIVERSITY   (India)

Author keywords

Amylase; Fungi; Isozymes; Zymography

Indexed keywords

FUNGI;

EID: 29744465300     PISSN: 0269915X     EISSN: 14740605     Source Type: Journal    
DOI: 10.1017/S0269915X05004015     Document Type: Article

References (18)

1. Banke, S., Frisvad, J. C. & Rosendal, S. (1997). Taxonomy of Penicillium chrysogenum and related xerophilic species based on isozyme analysis. Mycological Research 101: 617-624.
2. Bosland, P. W. & Williams, P. H. (1987). An evaluation of Fusarium oxysporum from crucifers based on pathogenicity, isozyme polymorphism, vegetative compatibility and geographic origin. Canadian Journal of Botany 65: 2067-2073.
3. Cruickshank, R. H. (1990). Pectin zymogram as criteria in taxonomy of Rhizoctonia. Mycological Research 94: 938-946.
4. Cruickshank, R. H. & Pitt, S. I. (1987). Identification of species in Penicilium subgenus Penicillium by enzyme electrophoresis. Mycologia 79: 614-620.
5. Elias, S. K. & Schneider, R. W. (1992). Genetic diversity within and among race and vegetative compatibility group of Fusarium oxysporum f. sp. lycopersici as determined by isozyme analysis Phytopathology 82: 1421-1427.
6. Grigg, R. & Lichtwardt, R. W. (1996). Isozyme patterns in cultured Harpellales. Mycologia 88: 219-229.
7. Gupta, A. K. & Gautam, S. P. (1993). Production of extracellular amylases by thermophilic and thermotolerent fungi. Cryptogamic Botany 3: 303-306.
8. Hames, B. D. (1990). One dimensional polyacrylamide gel electrophoresis In Gel Electrophoresis of Proteins: A Practical Approach II (ed. Hames, B.D. & Rickwood, D.), pp. 1-147. IRL Press: Oxford.
9. Jones, K. G. &Blackwell, M. (1996). Ribosomal DNA sequence analysis places the yeast like genus Symbiotaphrina within filamentous ascomycetes. Mycologia 88: 212-218.
10. Leuchtmann, A. & Clay, K. (1989). Isozyme variation in the fungus Aktinonella hypoxylon within and among population of its host grasses. Canadian Journal of Botany 67: 2600-2607.
11. Micales, J. A. (1986). Use of isozyme analysis in fungal taxonomy and genetics. Mycotaxon 27: 405-449.
12. Paterson, R. R. M., Bridge, P. D., Crosswhite, M. J. & Hawksworth, D. L. (1989). A reappraisal of the Penicillia using biochemical, physiological and morphological features, III. An evaluation of pectinase and amylase isozymes for species characterization. Journal of General Microbiology 135: 2979-2991.
13. Pierre, K. J. & Tung, K. K. (1984). a-Amylase: UV-method with maltotetraose. In: Methods of Enzymatic Analysis, Vol. 4, third edition (Bergmeyer, H.U., ed.), pp. 146-151. Wiley-VCH: Weinheim.
14. Rauscher, E. (1984). x-Amylase:colorimetric method. In: Methods of Enzymatic Analysis Vol. 4, third edition (ed. Bergmeyer, H.U.), pp. 157-161. Wiley-VCH: Weinheim.
15. Rojanavanich, V., Yoshiike, T., Tasuboi, R., Takamori, K. & Ogawa, H. (1990). Purification and characterization of extracellular proteinase from Hendersonula toruloidea. Infection and Immunity 58: 2850-2861.
16. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press: New York.
17. Skovgaard, K. & Rosendahl, S. (1998). Comparison of intra-and extracellular isozyme banding pattern of Fusarium oxysporum. Mycological Research 102: 1077-1084.
18. Upadhyay, Mukesh K., Pandey, A.K. & Rajak, R.C. (2003). Differentiation of Agaricus bisporus based on extracellular isozyme analysis. Journal of Basic and Applied Mycology 2: 6-9.