The effects of epithelial viability on stromal keratocyte apoptosis in porcine corneas stored in Optisol-GS

Cornea

Volumn 25, Issue 1, 2006, Pages 78-84

  Chang, Shu Wen ^a,b,c   Wang, Yao Horng ^d,e   Pang, Jong Hwei Su ^f  

^a FAR EASTERN MEMORIAL HOSPITAL   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

^c TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^d JEN TEH JUNIOR COLLEGE OF MEDICINE   (Taiwan)

^e CHINA MEDICAL UNIVERSITY   (Taiwan)

^f CHANG GUNG UNIVERSITY   (Taiwan)

Author keywords

Apoptosis; Cornea; Corneal epithelium; Keratocyte; Ligation mediated polymerase chain reaction; Preservation; Programmed cell death; Viability

Indexed keywords

BUFFER; DNA; DNA FRAGMENT; OPTISOL GS; PRESERVATION SOLUTION; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL DENSITY; CELL VIABILITY; CONTROLLED STUDY; CORNEA CELL; CORNEA PRESERVATION; CORNEA THICKNESS; CULTURE MEDIUM; DEBRIDEMENT; DNA DAMAGE; EPITHELIUM CELL; LIGATION MEDIATED POLYMERASE CHAIN REACTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SWINE; TIME; TISSUE PRESERVATION;

ANIMALS; APOPTOSIS; CELL COMMUNICATION; CELL COUNT; CELL SURVIVAL; CHONDROITIN SULFATES; COMPLEX MIXTURES; CORNEAL STROMA; CRYOPRESERVATION; DEBRIDEMENT; DEXTRANS; DNA; EPITHELIAL CELLS; EPITHELIUM, CORNEAL; FIBROBLASTS; GENTAMICINS; IN SITU NICK-END LABELING; ORGAN PRESERVATION; ORGAN PRESERVATION SOLUTIONS; POLYMERASE CHAIN REACTION; SWINE;

EID: 29744447892     PISSN: 02773740     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.ico.0000160970.58330.09     Document Type: Article

References (40)

1. Gao J, Gelber-Schwalb TA, Addeo JV, et al. Apoptosis in the rabbit cornea after photorefractive keratectomy. Cornea. 1997;16:200-208.
2. Ren H, Wilson G. Apoptosis in the corneal epithelium. Invest Ophthalmol Vis Sci. 1996;37:1017-1025.
3. Wilson SE, He YG, Weng J, et al. Epithelial injury induces keratocyte apoptosis: hypothesized role for the interleukin-1 system in the modulation of corneal tissue organization and wound healing. Exp Eye Res. 1996;62:325-327.
4. Wilson SE. Liu JJ, Mohan RR. Stromal-epithelial interactions in the cornea. Prog Retin Eye Res. 1999;18:293-309.
5. Orwin EJ, Hubel A. in vitro culture characteristics of corneal epithelial, endothelial, and keratocyte cells in a native collagen matrix. Tissue Eng. 2000;6:307-319.
6. Wang RG, Hjortdal JO, Ehlers N, et al. Histopathological findings in failed human epikeratophakia lenticules. Acta Ophthalmol (Copenh). 1994;72:363-368.
7. Meyer RF, Bobb KC. Corneal epithelium in penetrating keratoplasty. Am J Ophthalmol. 1980;90:142-147.
8. Lindstrom RL, Kaufman HE, Skelnik DL, et al. Optisol corneal storage medium. Am J Ophthalmol. 1992;114:345-356.
9. Kaufman HE, Beuerman RW, Steinemann TL, et al. Optisol corneal storage medium. Arch Ophthalmol. 1991;109:864-868.
10. Ardjomand N, Berghold A, Reich ME. Loss of corneal Langerhans cells during storage in organ culture medium, Optisol and McCarey-Kaufman medium. Eye. 1998;12:134-138.
11. Jablonski-Stiemke MM, Edelhauser HF. Storage of human corneas in dextran and chondroitin sulfate-based corneal storage medium: changes in stromal free sodium. Arch Ophthalmol. 1998;116:627-632.
12. Jablonski-Stiemke MM, Edelhauser HF. Comparison of rabbit and human corneas stored in Optisol-GS: changes in stromal sodium. Curr Eye Res. 1998;17:1044-1048.
13. Slack JW, Kangas TA, Edelhauser HF, et al. Comparison of corneal preservation media for corneal hydration and stromal proteoglycan loss. 14. Cornea. 1992;11:204-210.
14. Khodadoust AA, Silverstein AM. The survival and rejection of epithelium in experimental corneal transplants. Invest Ophthalmol. 1969;8:169.
15. Thoft RA, Friend J, Freedman H, et al. Corneal epithelial preservation. Arch Ophthalmol. 1975;93:357.
16. Mc Carey BE. Corneal storage and handling. In Kaufman HE, Zimmerman JT, eds. Current Concepts in Ophthalmology, Vol 5. St. Louis: Mosby: 1976: 134-164.
17. Thoft RA, Friend J. Corneal epithelial changes during midterm storage. Invest Ophthalmol. 1976;15:82.
18. Imbert D, Cullander C. Assessment of cornea viability by confocal laser scanning microscopy and MTT assay. Cornea. 1997;16:666-674.
19. Means TL, Geroski DH, L'Hernault N, et al. The corneal epithelium after Optisol-GS storage. Cornea. 1996;15:599-605.
20. Kristina S, Blaschke AJ, Chun J. Apoptotic DNA fragmentation is detected by a semi-quantitative ligation-mediated PCR of blunt DNA ends. Cell Death Differ. 1997;4:66-75.
21. Moller-Pedersen T, Ehlers N. A three-dimensional study of the human corneal keratocyte density. Curr Eye Res. 1995;14:459-464.
22. Patel SV, McLaren JW, Camp JJ, et al. Automated quantification of keratocyte density by using confocal microscopy in vivo. Invest Ophthalmol Vis Sci. 1999;40:320-326.
23. Prydal JI, Franc F, Dilly PN, et al. Keratocyte density and size in conscious humans by digital image analysis of confocal images. Eye. 1998;12:337-342.
24. Mustonen RK, McDonald MB, Srivannaboon S, et al. Normal human corneal cell populations evaluated by in vivo scanning slit confocal microscopy. Cornea. 1998;17:485-492.
25. Szerenyi KD, Wang X, Gabrielian K, et al. Keratocyte loss and repopulation of anterior corneal stroma after de-epithelialization. Arch Ophthalmol. 1994;112:973-976.
26. Hahnel C, Somodi S, Weiss DG, et al. The keratocyte network of human cornea: a three-dimensional study using confocal laser scanning fluorescence microscopy. Cornea. 2000;19:185-193.
27. Chang SW, Chi RF, Wu CC, et al. Benzalkonium chloride and gentamicin cause a leak in corneal epithelial cell membrane. Exp Eye Res. 2000;71:3-10.
28. Kim T, Palay DA, Lynn M. Donor factors associated with epithelial defects after penetrating keratoplasty. Cornea. 1996;15:451-456.
29. Chou L, Cohen EJ, Laibson PR, et al. Factors associated with epithelial defects after penetrating keratoplasty. Ophthalmic Surg. 1994;25:700-703.
30. Wollensak G, Spoerl E, Seiler T. Stress-strain measurements of human and porcine corneas after riboflavin-ultraviolet-A-induced cross-linking. J Cataract Refract Surg. 2003;29:1780-1785.
31. Hayashi S, Osawa T, Tohyama K. Comparative observations on corneas, with special reference to Bowman's layer and Descemet's membrane in mammals and amphibians. J Morphol. 2002;254:247-258.
32. Modis L Jr, Langenbucher A, Behrens A, et al. Flap quality in single versus multiple use of the same blade in the Flapmaker microkeratome. J Refract Surg. 2004;20:258-264.
33. Wollensak G, Sporl E, Reber F, et al. Corneal endothelial cytotoxicity of riboflavin/UVA treatment in vitro. Ophthalmic Res. 2003;35:324-328.
34. Viestenz A, Langenbucher A, Hofmann-Rummelt C, et al. Evaluation of corneal flap dimensions and cut quality using the SKBM automated microkeratome. J Cataract Refract Surg. 2003;29:825-831.
35. Seo KY, Wan XH, Jang JW, et al. Effect of microkeratome suction duration on corneal flap thickness and incision angle. J Refract Surg. 2002;18:715-719.
36. Poole CA, Brookes NH, Clover GM. Keratocyte networks visualized in the living cornea using vital dyes. J Cell Sci. 1993;106:685-692.
37. Hahnel C, Somodi S, Slowik C, et al. Fluorescence microscopy and three-dimensional imaging of the porcine keratocyte network. Graefes Arch. 1997;235:773-779.
38. Armitage WJ, Easty DL. Factors influencing the suitability of organ-cultured corneas for transplantation. Invest Ophthalmol Vis Sci. 1997;38:16-24.
39. Redbrake C, Salla S, Vonderhecken M, et al. Metabolic changes of the human donor cornea during organ culture. Acta Ophthalmol Scand. 1999;77:266-272.
40. Albon J, Tullo AB, Aktar S, et al. Apoptosis in the endothelium of human corneas for transplantation. Invest Ophthalmol Vis Sci. 2000;41:2887-2893.