Synthesis and testing of a novel fluorescence label for carboxyls in carbohydrates and cellulosics

Synlett

Volumn , Issue 20, 2005, Pages 3087-3090

  Bohrn, Rainer ^a   Potthast, Antje ^a   Rosenau, Thomas ^a   Sixta, Herbert ^b   Kosma, Paul ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b LENZING AG   (Austria)

Author keywords

Carbohydrates; Carboxyls; Fluorescence labeling; Polysaccharides; Uronic acids

Indexed keywords

CARBOHYDRATE; CARBOXYL GROUP; CELLULOSE; OLIGOSACCHARIDE; POLYSACCHARIDE; URONIC ACID;

ARTICLE; CARBOHYDRATE ANALYSIS; CHEMICAL REACTION; CHEMICAL STRUCTURE; DERIVATIZATION; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; HYDROLYSIS; LIGHT SCATTERING; OXIDATION; SYNTHESIS;

EID: 29744435536     PISSN: 09365214     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2005-921923     Document Type: Article

References (24)

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19. 4/Celite® (w/w = 1:1), which was washed with DMAc to a filtrate volume of 40 mL. The resulting red solution of FDAM (6) was used for labeling, and can be stored in the dark at 4 °C for up to 7 d.
20. Determined with an Agilent G1321A fluorescence detector.
21. 2-9″),
22. 20-21 (c 0.5, DMSO).
23. Synthesis and analytical data are reported elsewhere.
24. General Procedure for the Determination of Carboxyls in Cellulosic Pulps by Heterogeneous Fluorescence Labeling. Activated pulp, corresponding to 20 mg of dry pulp, was suspended in 0.1 M HCl and agitated for 20 s in a mixer. The pulp was washed with 0.1 M HCl, EtOH, DMAc, filtered off, transferred into a 4 mL vial, and suspended in 3 mL of DMAc. The FDAM solution (1 mL, approx. 0.125 M in DMAc) was added. The solution was agitated in a shaking bath for 7d at 40 °C. After derivatization, the pulp was filtered off, washed with DMAc, and transferred into a dry vial. Then, 1.6 mL of DMAc/LiCl (9%, m/v) were added, the sample was filtered through 0.45 μm filters after complete dissolution, and was analyzed by multi-detector GPC.