Isoelectric point determination of proteins and other macromolecules: Oscillating method

Computers in Biology and Medicine

Volumn 36, Issue 2, 2006, Pages 157-166

  Sillero, Antonio ^a,b   Maldonado, Andres ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b AUTONOMOUS UNIVERSITY OF MADRID   (Spain)

Author keywords

Acid base residues; Electric charge; pH; pI Theoretical determination; Proteins; Visual Basic

Indexed keywords

ACID BASE RESIDUES; ISOELECTRIC POINT; PI THEORETICAL DETERMINATION; VISUAL BASIC;

COMPUTER PROGRAMMING LANGUAGES; COMPUTER SOFTWARE; ELECTRIC CHARGE; MACROMOLECULES; PH EFFECTS;

PROTEINS;

ACID; BASE; PROTEIN;

ACCURACY; ARTICLE; AUTOANALYSIS; CALCULATION; COMPUTER ANALYSIS; COMPUTER PROGRAM; ISOELECTRIC POINT; MACROMOLECULE; OSCILLATION; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; THEORETICAL STUDY;

COMPUTER SIMULATION; ISOELECTRIC POINT; MACROMOLECULAR SUBSTANCES; MODELS, CHEMICAL; PROTEINS; SOFTWARE; SOFTWARE DESIGN;

EID: 29744432228     PISSN: 00104825     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.compbiomed.2004.09.006     Document Type: Article

References (13)

1. J.C. Cameselle, J.M. Ribeiro, and A. Sillero Derivation and use of a formula to calculate the net charge of acid-base compounds, Its application to aminoacids, proteins and nucleotides Biochem. Educ. 14 1986 131 136
2. A. Sillero, and J.M. Ribeiro Isoelectric points of proteins theoretical determination Anal. Biochem. 179 1989 319 325
3. J.M. Ribeiro, and A. Sillero An algorithm for the computer calculation of the coefficients of a polynomial that allows determination of isoelectric points of proteins and other macromolecules Comput. Biol. Med. 20 1990 235 242
4. J.M. Ribeiro, and A. Sillero A program to calculate the isoelectric point of macromolecules Comput. Biol. Med. 21 1991 131 141
5. J.M. Ribeiro, A. Ruiz, M.A. Sillero, and A. Sillero Theoretical isoelectric points and electric charges of mutated human haemoglobin subunits Clin. Chim. Acta 190 1990 189 197
6. D.S. Moore Amino acid and peptide net charges a simple calculational procedure Biochem. Educ. 13 1985 10 11
7. C.S. Patrickios, and E.N. Yamasaki Polypeptide amino acid composition and isoelectric point. II. Comparison between experiment and theory Anal. Biochem. 231 1995 82 91
8. R. Gras, M. Muller, E. Gasteiger, S. Gay, P.A. Binz, W. Bienvenut, C. Hoogland, J.C. Sanchez, A. Bairoch, D.F. Hochstrasser, and R.D. Appel Improving protein identification from peptide mass fingerprinting through a parameterized multi-level scoring algorithm and an optimized peak detection Electrophoresis 20 1999 3535 3550
9. G. Henriksson, A.K. Englund, G. Johansson, and P. Lundahl Calculation of the isoelectric points of native proteins with spreading of pKa values Electrophoresis 16 1995 1377 1380
10. K. Hiller, M. Schobert, C. Hundertmark, D. Jahn, R. Munch, and JVirGel Calculation of virtual two-dimensional protein gels Nucleic Acids Res. 31 2003 3862 3865
11. P.M. Larsen, L. Storgaard, and S.J. Fey Proteins present in bovine papillomavirus particles J. Virol. 61 1987 3596 3601
12. M.W. Reeves, M.C. Drummond, and M. Tager Partial purification and characterization of the multiple molecular forms of staphylococcal clotting activity (coagulase) J. Bacteriol. 148 1981 861 868
13. A. Santagostino, G. Giagnoni, P. Fumagalli, D. Pavesi, and E. Torretta Isoelectric point determination of human and camel beta-endorphin, alpha-endorphin and enkephalins Biochem. Biophys. Res. Commun. 104 1982 577 582