Isolation of C. elegans deletion mutants following ENU mutagenesis and thermostable restriction enzyme PCR screening

Molecular Biotechnology

Volumn 32, Issue 1, 2006, Pages 83-86

  Huang, Chunyi George ^a   Agre, Peter ^a   Strange, Kevin ^b   Lamitina, Todd ^b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Ethylnitrosourea; Mutation; Polymerase chain reaction

Indexed keywords

ETHYLNITROSOUREA; MUTATION; POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION (PCR);

ENZYME KINETICS; ENZYMES; GENES; GENETIC ENGINEERING; MUTAGENESIS; THERMODYNAMIC STABILITY;

MICROORGANISMS;

ETHYLNITROSOUREA; RESTRICTION ENDONUCLEASE;

ARTICLE; CAENORHABDITIS ELEGANS; GENE DELETION; GENE ISOLATION; GENETIC SCREENING; MUTAGENESIS; MUTANT; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; THERMOSTABILITY;

ALKYLATING AGENTS; ANIMALS; CAENORHABDITIS ELEGANS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, HELMINTH; ETHYLNITROSOUREA; GENE DELETION; GENES, HELMINTH; MUTAGENESIS; MUTATION; POLYMERASE CHAIN REACTION;

CAENORHABDITIS ELEGANS;

EID: 29644431851     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1186/1471-2105-7-83     Document Type: Article

References (7)

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