Chromosome aberrations, sister chromatid exchanges (SCE) and cell division kinetics in human lymphocytes exposed in vitro to purified trypsin inhibitor from Ascaris

Journal of Applied Genetics

Volumn 45, Issue 2, 2004, Pages 265-274

  Błaszkowska, Joanna ^a   Bratkowska, Wanda ^a   Łopaczyńska, Dobrosława ^a   Strózyński, Henryk ^a   Ferenc, Tomasz ^a  

^a MEDICAL UNIVERSITY OF LODZ   (Poland)

Author keywords

Ascaris; Chromosome aberrations; Human lymphocytes; Mitotic index; Replication index; Sister chromatid exchange; Trypsin inhibitor

Indexed keywords

SEPHADEX; TRYPSIN INHIBITOR; HELMINTH PROTEIN; INSECT PROTEIN; TRYPSIN INHIBITOR, ASCARIS;

ADULT; ARTICLE; ASCARIS SUUM; CELL CULTURE; CELL DIVISION; CELL KINETICS; CHROMOSOME ABERRATION; CONTROLLED STUDY; GEL FILTRATION; GENOTOXICITY; HUMAN; LYMPHOCYTE; MALE; METABOLIC ACTIVATION; MITOSIS INDEX; NONHUMAN; NORMAL HUMAN; PERIPHERAL LYMPHOCYTE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ISOLATION; PROTEIN PURIFICATION; SISTER CHROMATID EXCHANGE; ANIMAL; ASCARIS; CULTURE TECHNIQUE; DRUG EFFECT; KINETICS; MUTAGEN TESTING; PHYSIOLOGY;

ASCARIS; ASCARIS SUUM;

ANIMALS; ASCARIS; CELL CULTURE TECHNIQUES; CELL DIVISION; CHROMOSOME ABERRATIONS; HELMINTH PROTEINS; HUMANS; INSECT PROTEINS; KINETICS; LYMPHOCYTES; MUTAGENICITY TESTS; SISTER CHROMATID EXCHANGE; TRYPSIN INHIBITORS;

EID: 2942738913     PISSN: 12341983     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. BARANOVA F.S., BERMAN A.A., ZARETSKAYA Y.M. (1980). Effect of alpha protease inhibitor (alpha-antitrypsin) on the intensity of transformation of phytohemagglutinin-stimulated lymphocytes. Biull. Eksp. Biol. Med. 89: 44-48.
2. BEKISH V.J. (1999). Mutagenic activity of antigens from Ascaris suum tissue. Zdrovookhronene 6: 17-19 (in Russian).
3. BEKISH V.J. (2000). The influence of larvae metabolites of Ascaris suum on the genome of host in reinvasion. Acta Parasitol. 45: 243.
4. BEKISH V.J. (2001). The alterations in genetic apparatus of somatic and generative cells of the host caused by helminths metabolites. Wiad. Parazytol. 47: 891-896.
5. BEKISH O.-J.L., BEKISH V.J. (2000). Nematode metabolites as mutagens of somatic and generative cells of host. Acta Parasitol. 45: 244.
6. BłASZKOWSKA J. (1998a). Embryotoxic and teratogenic action of trypsin inhibitor of Ascaris lumbricoides in mice. Acta Parasitol. 43: 103-108.
7. BłASZKOWSKA J. (1998b). The effect of Ascaris suum homogenate and its proteolysis inhibitors on chicken embryos. Helminthologia 35: 37-42.
8. BłASZKOWSKA J. (1999a). Disturbances of mouse pregnancy after injection of Ascaris trypsin inhibitor during early organogenesis. Zool. Pol. 44: 23-36.
9. BłASZKOWSKA J. (1999b). The effect of Ascaris tegumental homogenate and the α-chymotrypsin inhibitor isolated from the nematode on mouse pregnancy. Helminthologia 36: 225-234.
10. BłASZKOWSKA J. (2001). Disturbances of mouse pregnancy after injection of Ascaris chymotrypsin inhibitor during early organogenesis. Helminthologia 38:15-21.
11. BREIT S.N., LUCKHURST E., PENNY R. (1983). The effect of alpha 1 antitrypsin on the proliferative response of human peripheral blood lymphocytes. J. Immunol. 130: 681-686.
12. EPSTEIN S.S., ARNOLD E., ANDREA J., BASS W., BISHOP Y. (1972). Detection of chemical mutagens by the dominant tethal assay in the mouse. Toxicol. Appl. Pharmacol. 23: 288-325.
13. FERENC T., JANIK-SIECHOWICZ E., BRATKOWSKA W., ŁOPACZYŃSKA D., STRÓŻYŃSKI H., DENYS A., MORDALSKA A. (1999). Genotoxicity assessment of new synthesized acridine derivative - 3,6-diamino-10-methyl-9,10-dihydroacridine. Mut. Res. 444: 463-470.
14. FERREIRA A.P., FAQUM E.S., ABRAHAMSOHN I.A., MACEDO M.S. (1995). Immunization with Ascaris suum extract impairs T cell functions in mice. Cell. Immunol. 162: 202-210.
15. GOODMAN R.B., PEANASKY R.J. (1982). Isolation of the trypsin inhibitors in Ascaris lumbricoides var. suum using affinity chromatography. Anal. Biochem. 120:387-393.
16. GRASBERGER B.L., CLORE G.M., GRONENBORN A.M. (1994). High-resolution structure of Ascaris trypsin inhibitor in solution: direct evidence for a pH-induced conformational transition in the reactive site. Structure 2: 669-678.
17. GRUTTER M.G. (1994). Proteinase inhibitors: another new fold. Structure 2: 575-576.
18. HUANG K., PEANASKY R.J., JAMES M.N.G. (1994). The molecular structure of the complex of the chymotrypsin/elastase inhibitor from Ascaris lumbricoides suum with porcine elastase. Structure 2: 679-689.
19. ISCN (1995). Chromosome breakage. In: An international system for human cytogenetics nomenclature, recommendations of the International Standing Committee on Human Cytogenetic Nomenclature (F. Mitelman, ed.), Cytogenet. Cell. Genet. Karger.: 75-77.
20. JUHASZ S., MATSKASI J. (1979). Proteolytic enzymes and enzyme inhibitors in Ascaris suum. III. In vitro study of proteinase inactivation by the parasite. Acta Veter. Acad. Sci. Hung. 27: 93-97.
21. KASSELL B. (1970). Methods of Enzymology (G. Perlmann, L. Lorand, eds.), Academic Press, New York 19: 872-883.
22. KADłUBOWSKI R., OCHȨCKA A. (1986). Proteases and their inhibitors in host-parasite system. I. The effect of Ascaris inhibitors on the selected parameters of human plasma fibrinolysis. Acta Parasitol. Pol. 30: 179-186.
23. KADŁUBOWSKI R., OCHȨCKA A., WÓJCIK A., BŁASZKOWSKA J. (1991). Proteolysis inhibitors from Ascaris lumbricoides: fact or fiction? Wiad. Parazytol. 37: 423-432.
24. KADŁUBOWSKI R., WÓJCIK A. (1990). Proteases and their inhibitors in host-parasite system. IX. The effect of Ascaris trypsin inhibitor on urine proteolytic activity following treatment with Ascaris tegument homogenate and in experimental larval ascariasis. Acta Parasitol. Pol. 35: 61-68.
25. KUCICH U., PEANASKY R.J. (1970). Trypsin inhibitor from Ascaris lumbricoides var. suis. Biochim. Biophys. Acta 200: 47-57.
26. LEE D.E., XIE C.Y. (1995). IgE regulation by nematodes: The body fluid of Ascaris contains a B-cell mitogen. J. Allergy. Clin. Immunol. 96: 1246-1254.
27. LIPSKI J.J., BERNINGER R.W., HYMAN L.R., TALAMA R.C. (1979). Presence of alpha-1-antitrypsin on mitogen-stimulated human lymphocytes. J. Immunol. 122: 24-26.
28. LOHRISCH I., SCHERBAUM I., GRUHN R., AMBROSIUS H., HAUSTEIN U.F., HERRMANN K. (1981). Alpha 1-antitrypsin: production and binding of in vitro stimulated peripheral blood lymphocytes. Acta Biol. Med. Ger. 40: 1767-1773.
29. MARTZEN M.R., GEISE GL., HOGAN R.J., PEANASKY R.J. (1985). Ascaris suum: localization of the host proteases and parasite proteinase inhibitors in cross-section using immunochemical and fluorescent probe technique. Exp. Parasitol. 60: 139-149.
30. MAZUMDER D.N., SANDRA A., DUTTA G. (1992). Hepatic lesions caused by excretory and secretory products of Ascaris lumbricoides in golden hamster. Indian J. Gastroenterol. 11: 117-120.
31. MUTO R., IMAI S., TEZUKA H., FURUHASHI Y., FUJITA K. (2001). The biological activity of ABA-1-like protein from Ascaris lumbricoides. J. Med. Dent. Sci. 48: 95-104.
32. OCHȨCKA A., KADLUBOWSKI R. (1986). Proteases and their inhibitors in host-parasite system. III. Effect of the parasite (Ascaris) inhibitors on the proteolytic activity. Acta Parasitol. Pol. 30: 97-106.
33. OTLEWSKI J., KROWARSCH D., APOSTOLUK A. (1999). Protein inhibitors of serine proteases. Acta Bioch. Pol. 46: 531-565.
34. RHOAD M.L., FETTERER R.A., URBAN J.F. (1997). Secretion of an aminopeptidase during transition of third to fourth-stage larvae of Ascaris suum. J. Parasitol. 83: 780-784
35. ROJAS E., MONTERO R., HERRERA L.A., SORDO M., GONSEBATT M.E., RODRIGUES R., OSTROSKY-WEGMAN P. (1992). Are mitotic index and lymphocyte proliferation kinetics reproducible endpoints in genetic toxicology testing? Mutat. Res. 282: 283-286.
36. ROLA F.H., PUDLES J.A. (1966). Studies on the chymotryptic inhibitor from Ascaris lumbricoides var. suum, purification and properties. Arch. Biochem. Biophys. 113: 134-142.
37. PUDLES J.A., ROLA F.H., MATIDA A.K. (1967). Studies on the proteolytic inhibitors from Ascaris lumbricoides var. suum. II. Purification, properties and chemical modification of the trypsin inhibitor. Arch. Biochem. Biophys. 120: 594-601.
38. SASAGAWA S., SUZUKU K., FUJIKURA T. (1987). Ascaris suum: Human lymphocyte mitogenic factor content. Exp. Parasitol. 64: 71-77.
39. SHUBBER E.K., SALIN H. (1987). Cytogenic studies on blood lymphocytes from patients with Schistosoma mansoni. Jap. J. Med. Sci. Biol. 4: 137-145.
40. SOARES M.F., MOTA I., MACEDO M.S. (1992). Isolation of Ascaris suum components with suppress IgE antibody responsive. Int. Arch. Allergy Immunol. 97: 37-43.