A very rapid multiplex polymerase chain reaction for simultaneous detection of factor V Leiden and G20210A prothrombin mutation [1]

Clinical and Laboratory Haematology

Volumn 26, Issue 3, 2004, Pages 239-240

  Correa M F B ^a   Rezende, S M ^a  

^a Hemominas Foundation   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 5 LEIDEN; PROTHROMBIN;

DNA EXTRACTION; ELECTROPHORESIS; GENE MUTATION; GENOTYPE; LETTER; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

DNA MUTATIONAL ANALYSIS; FACTOR V; HUMANS; POINT MUTATION; POLYMERASE CHAIN REACTION; PROTHROMBIN; QUALITY CONTROL; TIME FACTORS;

EID: 2942604607     PISSN: 01419854     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2257.2004.00603.x     Document Type: Letter

References (6)

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2. Gomez E., van der Poel S.C.P.A.M., Jansen J.H., van der Reijden B.A. & Löwenberg B. (1998) Rapid simultaneous screening of factor V Leiden and G20210A prothrombin variant by multiplex polymerase chain reaction on whole blood. Blood 91, 2208-2209.
3. Huber S., McMaster K.J. & Voelkerding K.V. (2000) Analytical evaluation of primer engineered multiplex polymerase chain reaction-restriction fragment length polymorphism for detection of factor V Leiden and prothrombin G20210A. Journal of Molecular Diagnostic 2, 153-157.
4. Makris M., Preston F.E., Beauchamp N.J., Cooper P.C., Daly M.E., Hamp K.K., Bayliss P., Peake I.R. & Miller G.J. (1997) Co-inheritance of the 20210 A allele of the prothrombin gene increases the risk of thrombosis in subjects with familial thrombophilia. Thrombosis and Haemostasis 78, 1426-1429.
5. Muriel G., Christine B., Phillipe J., Christophe M., Francois S.J. & Patricia A.M. (1998) An even easier method for one-step detection of both FV Leiden and FII G20210A transition. Blood 92, 3478-3479.
6. Poort S.R., Rosendaal F.R., Reitsma P.H. & Bertina R.M. (1996) A common genetic variation in the 3′-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. Blood 88, 3698-3703.