The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins

Cell Research

Volumn 13, Issue 5, 2003, Pages 351-359

  Shu, Bing Zhang ^a   Huang, Jian ^a   Zhao, Hui ^a   Zhang, Yi ^b   Chun, Hui Hou ^a   Xiao, Dong Cheng ^a   Jiang, Chu ^a   Min, Qian Li ^b   Hu, Jun ^b   Qian, Ruo Lan ^a  

^a Shanghai Jiao Tong University Affiliated Sixth People s Hospital   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

Atomic force microscopy; HMG proteins (HMG1 2 and HMG14 17); Human b globin gene; In vitro reconstitution; Nucleosome

Indexed keywords

DNA; GLOBIN; HIGH MOBILITY GROUP B1 PROTEIN; HIGH MOBILITY GROUP B2 PROTEIN; HIGH MOBILITY GROUP N1 PROTEIN; HIGH MOBILITY GROUP N2 PROTEIN; HIGH MOBILITY GROUP PROTEIN; HISTONE;

ANIMAL; ARTICLE; ATOMIC FORCE MICROSCOPY; CELL NUCLEUS; CHICKEN; CHROMATIN; GENE EXPRESSION REGULATION; GENETICS; HUMAN; IN VITRO STUDY; METABOLISM; METHODOLOGY; NUCLEOSOME; PROTEIN BINDING; ULTRASTRUCTURE;

ANIMALS; CELL NUCLEUS; CHICKENS; CHROMATIN; DNA; GENE EXPRESSION REGULATION; GLOBINS; HIGH MOBILITY GROUP PROTEINS; HISTONES; HMGB1 PROTEIN; HMGB2 PROTEIN; HMGN1 PROTEIN; HMGN2 PROTEIN; HUMANS; MICROSCOPY, ATOMIC FORCE; NUCLEOSOMES; PROTEIN BINDING;

EID: 2942596397     PISSN: 10010602     EISSN: 17487838     Source Type: Journal    
DOI: 10.1038/sj.cr.7290180     Document Type: Article

References (22)

1. Qian RL, Liu ZX, Zhou MY, Xie HY, Jiang C, Yan ZJ, Li MQ, Zhang Y, and Hu J. Visualization of chromatin folding patterns in chicken erythrocytes by atomic force microscopy (AFM). Cell Research 1997; 7:143-50.
2. Zhao H, Zhang Y, Zhang SB, Jiang C, He QY, Li MQ, and Qian RL. The structure of the nucleosome core particle of chromatin in chicken erythrocytes visualized by using atomic force microscopy. Cell Research 1999; 9:255-60.
3. Berg, PE, Williams DM, Qian RL, Cohen Rb, Cao SX, Mittelman M, and Schechter AN. A common protein binds to two silencers 5′to the human b-globin gene. Nucleic Acids Res 1989; 17:8833-52.
4. Lutter LC. Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix. J Mol Biol 1978; 124:391-420.
5. Drew HR and Calladine CR. Sequence-specific positioning of core histones on an 860 base-pair DNA, experiment and theory. J Mol Biol 1987; 195:143-73.
6. Rhodes D and Laskey RA. Assembly of nucleosomes and chromatin in vitro. Methods Enzymol 1989; 170:575-85.
7. Goodwin GH and Johns EW.Isolation and characterization of two calf-thymus chromotin non-histone proteins with high contents of acidic and basic amino acids. Eur J Biochem 1973; 40:215-9.
8. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248-54.
9. Yan ZJ, Chen YD, and Qian RL. Developmental stage-specific factors in the mouse hematopoietic tissues binding to the 5′-flanking cis-acting element of human e-globin gene. Chinese Science Bulletin 1995; 40:778-83.
10. Kornberg RD. Structure of chromatin. Annu Rev Biochem 1977; 46:931-54.
11. Luger K, Mader AW, Richmond RK, Sargent DF, and Richmond TJ. Crystal structure of the nucleosome core particle at 2.8 ? resolution. Nature 1997; 389:251-60.
12. Allen MJ, Dong XF, O'Neill TE, Yau P, Kowalczykowski SC, Gatewood J, Balhorn R and Bradbury EM. Atomic force microscope measurement of nucleosome cores assembled along defined DNA sequences. Biochemistry 1993; 32:8390-6.
13. Leuba SH, Bustamante C, Van holde K and Zlatanova J. Linker histone tails and N-tails of histone H3 are redundant: Scanning force microscopy studies of reconstituted fibers. Biophys J 1998; 74:2830-9.
14. Bustin M, and Reeves R. High mobility group chromosomal proteins: architectural components that facilitate chromatin function. Prog Nucleic Acid Res Mol Biol 1996; 54:35-100.
15. Michael B and Raymond R. High-mobility-group chromosomal proteins: architectural components that facilitate chromatin function. Progress in Nucleic Acid Research and Molecular Biology 1996; 54:35-101.
16. Sandeen G, Wood WI and Felsenfeld G. The interaction of high mobility proteins HMG14 and 17 with nucleosomes. Nucleic Acids Res 1980; 8:3757-78.
17. Druckmann S, Mendelson E, Landsman D and Bustin M. Immunofractionation of DNA sequences associated with HMG 17 in chromatin. Exp Cell Research 1986; 166:486-96.
18. Brotherton TW, Reneker J and Ginder GD. Binding of HMG 17 to mononucleosomes of the avian b-globin gene cluster in erythroid and non-erythroid cells. Nucleic Acids Res 1990; 18:2011-6.
19. Postnikov YV, Shick VV, Belyavsky AV, Khrapko KR, Brodolin KL, Nikolskaya TA and Mirzabekov AD. Distribution of high mobility group proteins 1/2, E and 14/17 and linker histones H1 and H5 on transcribed and non-transcribed regions of chicken erythrocyte chromatin. Nucleic Acids Res 1991; 19:717-25.
20. Trieschmann L, Alfonso PJ, Crippa MP, Wolffe AP and Bustin M. Incorporation of chromosomal proteins HMG-14/HMG-17 into nascent nucleosomes induces an extended chromatin conformation and enhances the utilization of active transcription complexes. EMBO J 1995; 14:1478-89.
21. Yan ZJ, Chen YD and Qian RL. Interactions between HMG proteins and 5′flanking cis-acting elements of human e-globin gene. Acta Biochimica et Biophysica Sinica 1998; 30:471-5.
22. Qian RL, Chen YD, Hu YL, Li MQ, Wang ZH and Xu YL. Interaction between HMG proteins (1+2) and the negative regulatory region 1 (NCR1) in the 5′-flanking sequence of the human b-globin gene. Science in China (series B) 1993; 36:933-9.