Development of an LC-MS based enzyme activity assay for MurC: Application to evaluation of inhibitors and kinetic analysis

Journal of Pharmaceutical and Biomedical Analysis

Volumn 35, Issue 4, 2004, Pages 817-828

  Deng, Gejing ^a   Gu, Rong Fang ^a   Marmor, Stephen ^a   Fisher, Stewart L ^a   Jahic, Haris ^a   Sanyal, Gautam ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

, methyleneadenosine 5 triphosphate; adenosine 5 diphosphate; adenosine 5 triphosphate; ADP; AMP PCP; ATP; MurC; UDP N acetylmuramyl L alanine ligase; UNAM; UNAM Ala; uridine diphosphate N acetylmuramic acid

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; BACTERIAL ENZYME; BETA,GAMMA METHYLENEADENOSINE TRIPHOSPHATE; LIGASE; MALACHITE GREEN; PEPTIDOGLYCAN; PHOSPHATE; PHOSPHINATE INHIBITOR 1; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLMURAMYLALANINE LIGASE;

ACCURACY; ANALYTIC METHOD; ARTICLE; ARTIFACT; COMPARATIVE STUDY; CONTROLLED STUDY; DRUG ANALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME MECHANISM; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; INTERMETHOD COMPARISON; KINETICS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; SEPARATION TECHNIQUE; TECHNIQUE;

CHROMATOGRAPHY, LIQUID; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; ENZYME ACTIVATION; ENZYME INHIBITORS; ESCHERICHIA COLI PROTEINS; KINETICS; MASS SPECTROMETRY; PEPTIDE SYNTHASES; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY;

EID: 2942552549     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jpba.2004.02.029     Document Type: Article

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