A novel and convenient relative quantitative method of fluorescence real time RT-PCR assay based on slope of standard curve

Progress in Biochemistry and Biophysics

Volumn 32, Issue 9, 2005, Pages 883-888

  Zhang, Chi Yu ^a   Xu, Shun Gao ^a   Huang, Xin Xiang ^a  

^a JIANGSU UNIVERSITY   (China)

Author keywords

Absolute quantification; Fluorescence real time quantitative RT PCR; mRNA quantification; Relative quantification; Slope; Standard curve

Indexed keywords

EID: 29344466037     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Bustin S A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol, 2000, 25(2): 169-193
2. Giulietti A, Overbergh L, Valckx D, et al. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods, 2001, 25 (4): 386-401
3. Zhang Y, Shen Q. Prog Biochem Biophys, 2003, 30 (3): 474-477
4. Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) Method. Methods, 2001, 25 (4): 402-408
5. Xu S G, Huang X X, Zhou L P. J Jiangsu Univ (Med Sci), 2005, 15 (1): 21-24
6. Zhang C Y, Zhang G H, Yang M, et al. Chin J Biochem Mol Biol, 2004, 20 (3): 387-392
7. Liu W, Saint D A. A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics. Anal Biochem, 2002, 302 (1): 52-59