Substrate-assisted movement of the catalytic Lys 215 during domain closure: Site-directed mutagenesis studies of human 3-phosphoglycerate kinase

Biochemistry

Volumn 44, Issue 51, 2005, Pages 16853-16865

  Flachner, Beáta ^a   Varga, Andrea ^a   Szabó, Judit ^a   Barna, László ^a   Hajdú, István ^a   Gyimesi, Gergely ^a   Závodszky, Péter ^a   Vas, Mária ^a,b  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b INSTITUTE OF ENZYMOLOGY   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; CATALYST ACTIVITY; CONFORMATIONS; ENZYME KINETICS; MUTAGENESIS;

ANION-CAUSED ACTIVATION; CATALYTIC RESIDUES;

ENZYMES;

ADENOSINE DIPHOSPHATE; ADENOSINE DIPHOSPHATE MAGNESIUM; ADENOSINE TRIPHOSPHATE MAGNESIUM; ALANINE; ARGININE; LYSINE; MUTANT PROTEIN; PHOSPHATE; PHOSPHOGLYCERATE KINASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; HUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SITE DIRECTED MUTAGENESIS;

ADENOSINE TRIPHOSPHATE; ALLOSTERIC REGULATION; ALLOSTERIC SITE; AMINO ACID SUBSTITUTION; CALORIMETRY, DIFFERENTIAL SCANNING; CATALYSIS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; DIPHOSPHATES; DIPHOSPHOGLYCERIC ACIDS; ENZYME STABILITY; GLYCERIC ACIDS; HUMANS; KINETICS; LYSINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHOSPHOGLYCERATE KINASE; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; SULFHYDRYL COMPOUNDS;

EID: 29344443515     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051726g     Document Type: Article

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