Implications of surface charge and curvature for the binding orientation of Thermomyces lanuginosus lipase on negatively charged or zwitterionic phospholipid vesicles as studied by ESR spectroscopy

Biochemistry

Volumn 44, Issue 50, 2005, Pages 16658-16671

  Hedin, Eva M K ^a   Høyrup, Pernille ^b   Patkar, Shamkant A ^c   Vind, Jesper ^c   Svendsen, Allan ^c   Hult, Karl ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c NOVOZYMES A S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; ELECTRON SPIN RESONANCE SPECTROSCOPY; FLUORESCENCE; INTERFACES (MATERIALS); PHOSPHOLIPIDS; QUENCHING;

FLUORESCENCE QUENCHING; LIPID MEMBRANE; THERMOMYCES LANUGINOSUS LIPASE (TLL); ZWITTERIONIC PHOSPHOLIPID VESICLES;

ENZYMES;

1 PALMITOYL 2 OLEOYLGLYCERO 3 PHOSPHATIDYLGLYCEROL; 2 OLEOYL 1 PALMITOYLPHOSPHATIDYLCHOLINE; AMPHOLYTE; CHROMIUM; OXALIC ACID; PHOSPHATIDYLGLYCEROL; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; ELECTRON SPIN RESONANCE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; FLUORESCENCE; FUNGUS; LIPID VESICLE; MEMBRANE BINDING; PHOSPHOLIPID VESICLE; PRIORITY JOURNAL; SPIN LABELING; SURFACE CHARGE; THERMOMYCES LANUGINOSUS;

ASCOMYCOTA; ELECTRON SPIN RESONANCE SPECTROSCOPY; FLUORESCENCE; LIPASE; MODELS, MOLECULAR; MUTATION; PHOSPHOLIPIDS; PROTEIN CONFORMATION; SPIN LABELS; SURFACE PROPERTIES;

FUNGI; THERMOMYCES LANUGINOSUS;

EID: 29244471711     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051478o     Document Type: Article

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