Simultaneous observation of enzyme surface diffusion and surface reaction using microfluidic patterning of substrate surfaces

Analytical Chemistry

Volumn 77, Issue 24, 2005, Pages 8146-8150

  Roy, Shaunak ^a   Thomas, Jerry M ^a   Holmes, Elizabeth A ^a   Kellis Jr , James T ^c   Poulose, A J ^c   Robertson, Channing R ^a   Gast, Alice P ^b  

^a Stanford University   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c GENENCOR INTERNATIONAL INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; MICROFLUIDIC PATTERNING; SUBSTRATE SURFACES; SURFACE DIFFUSION;

DIFFUSION; FLUIDIC DEVICES; PROTEINS; REACTION KINETICS; SUBSTRATES; SURFACE STRUCTURE;

ENZYMES;

BOVINE SERUM ALBUMIN; ENZYME; GLUTARALDEHYDE; PROTEINASE;

ADSORPTION; ARTICLE; DIFFUSION; DIFFUSION COEFFICIENT; ENZYME ACTIVITY; ENZYME SUBSTRATE; FLUORESCENCE; MICHAELIS MENTEN KINETICS; MICROFLUIDICS; MODEL; OBSERVATION; SURFACE PROPERTY;

ANIMALS; CATTLE; DIFFUSION; ENZYMES; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; MICROFLUIDIC ANALYTICAL TECHNIQUES; SERUM ALBUMIN, BOVINE; SUBTILISIN; SURFACE PROPERTIES;

EID: 29244455651     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac050544p     Document Type: Article

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