Smilaxin, a novel protein with immunostimulatory, antiproliferative, and HIV-1-reverse transcriptase inhibitory activities from fresh Smilax glabra rhizomes

Biochemical and Biophysical Research Communications

Volumn 340, Issue 1, 2006, Pages 118-124

  Chu, K T ^a   Ng, T B ^a  

^a and Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics   (Hong Kong)

Author keywords

Antiproliferative; Glycoprotein; Immunostimulatory; Isolation; Protein; Rhizomes; Smilax glabra

Indexed keywords

AMINO ACID; CELLULOSE; CONCANAVALIN A; DIETHYLAMINOETHYL CELLULOSE; GLUCOSE; NITRIC OXIDE; PLANT EXTRACT; RNA DIRECTED DNA POLYMERASE; SEPHAROSE; SMILAX GLABRA EXTRACT; SMILAXIN; THYMIDINE; TRITIUM; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ADIPOSE TISSUE; ADSORPTION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTIFUNGAL ACTIVITY; ARTICLE; BONE MARROW CELL; CELL PROLIFERATION; CONTROLLED STUDY; DRUG ACTIVITY; DRUG ISOLATION; DRUG MECHANISM; DRUG PURIFICATION; DRUG UPTAKE; ENZYME ACTIVITY; ENZYME INHIBITION; FAST PROTEIN LIQUID CHROMATOGRAPHY; GEL FILTRATION; GLUCOSE TRANSPORT; HEMAGGLUTINATION; IC 50; IMMUNOSTIMULATION; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PERITONEUM MACROPHAGE; PRIORITY JOURNAL; RAT; RHIZOME; SARSAPARILLA; SMILAX GLABRA; SPLEEN CELL; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS; BONE MARROW CELLS; CELL LINE, TUMOR; CELL PROLIFERATION; DOSE-RESPONSE RELATIONSHIP, DRUG; HIV-1 REVERSE TRANSCRIPTASE; HUMANS; IMMUNOLOGIC FACTORS; MALE; MICE; NEOPLASMS; PLANT EXTRACTS; PLANT PROTEINS; PLANT ROOTS; RATS; SMILAX; SPLEEN;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS 1; MURINAE; SMILAX ARISTOLOCHIIFOLIA; SMILAX GLABRA;

EID: 29244432770     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.12.010     Document Type: Article

References (51)

1. S.C. Cheung, N.H. Li, Chinese Medicinal Herbs of Hong Kong, vol. 1, Commercial Press, Hong Kong, 1997.
2. T. Chen, J. Li, J. Cao, Q. Xu, K. Komatsu, and T. Namba A new flavanone isolated from rhizoma smilacis glabrae and the structural requirements of its derivatives for preventing immunological hepatocyte damage Planta Med. 65 1999 56 59
3. T. Chen, J.X. Li, and Q. Xu Phenylpropanoid glycosides from Smilax glabra Phytochemistry 53 2000 1051 1055
4. T. Fukunaga, T. Miura, K. Furuta, and A. Kato Hypoglycemic effect of the rhizomes of Smilax glabra in normal and diabetic mice Biol. Pharm. Bull. 20 1997 44 46
5. J. Jiang, and Q. Xu Immunomodulatory activity of the aqueous extract from rhizome of Smilax glabra in the later phase of adjuvant-induced arthritis in rats J. Ethnopharmacol. 85 2003 53 59
6. S.E. Lee, E.M. Ju, and J.H. Kim Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root Exp. Mol. Med. 33 2001 263 268
7. Y.W. Lam, and T.B. Ng A monomeric mannose-binding lectin from inner shoots of the edible chive (Allium tuberosum) J. Protein Chem. 20 2001 361 366
8. Y. Oda, and K. Minami Isolation and characterization of a lectin from tulip bulbs Tulipa gesneriana Eur. J. Biochem. 159 1986 239 245
9. E.J.M. Van Damme, K. Smeets, J. Engelborghs, H. Aelbers, J. Balzarini, A. Pusztai, F. Van Leuven, I.J. Goldstein, and W.J. Peumans Cloning and characterization of the lectin cDNA clones from onion, shallot, and leek Plant Mol. Biol. 23 1993 365 376
10. E.J.M. Van Damme, K. Smeets, S. Torrekens, F. Van Leuven, I.J. Goldstein, and W.J. Peumans The mannose-specific lectins from ramsons (Allium ursinum L.) are enclosed by three sets of genes Eur. J. Biochem. 217 1993 123 139
11. E.J.M. Van Damme, J. Balzarini, K. Smeets, F. Van Leuven, and W.J. Peumans The monomeric and dimeric mannose binding proteins from the Orchidaceae species Listera ovata and Epipactis helleborine: sequence homologies and differences in biological activities Glycoconjugate J. 11 1994 321 332
12. E.J.M. Van Damme, K. Smeets, S. Torrekens, F. Van Leuven, and W.J. Peumans Characterization and molecular cloning of the mannose binding lectins from three Orchidaceae species: Listera ovata, Epipactis helleborine, and Cymbidium hybrid Eur. J. Biochem. 221 1994 769 777
13. E.J.M. Van Damme, K. Goossens, K. Smeets, F. Van Leuven, P. Verheart, and W.J. Peumans The major tuber storage protein of Araceae species is a lectin Plant Physiol. 107 1995 1147 1158
14. K. Smeets, E.J. Van Damme, P. Verhaert, A. Barre, P. Rouge, F. Van Leuven, and W.J. Peumans Isolation, characterization and molecular cloning of the mannose-binding lectins from leaves and roots of garlic (Allium sativum L.) Plant Mol. Biol. 33 1997 223 234
15. T.B. Ng, and Y.L. Yu Isolation of a novel heterodimeric agglutinin from rhizomes of Smilax glabra, the Chinese medicinal material tufuling Int. J. Biochem. Cell Biol. 33 2001 269 277
16. L.S. Ooi, T.B. Ng, Y. Geng, and V.E. Ooi Lectins from bulbs of the Chinese daffodil Narcissus tazetta (family Amaryllidaceae) Biochem. Cell Biol. 78 2000 463 468
17. H.X. Wang, and T.B. Ng Purification of allivin, a novel antifungal protein from bulbs of the round-cloved garlic Life Sci. 70 2001 357 365
18. B.P.A. Cammue, K. Thevissen, M. Hendriks, K. Eggermont, I.J. Goderis, P. Proost, I. Van Damme, R.W. Osborn, F. Guerbette, J.C Kader, and W.F. Broekaert A potent antimicrobiol protein from onion seeds showing sequence homology to plant lipid transfer proteins Plant Physiol. 109 1995 445 455
19. L.X. Xia, and T.B. Ng Isolation of alliumin, a novel protein with antimicrobial and antiproliferative activities from multiple-cloved garlic bulbs Peptides 26 2005 177 183
20. H.X. Wang, and T.B. Ng Ascalin, a new antifungal peptide with human immunodeficiency virus type 1 reverse transcriptase-inhibiting activity from shallot bulbs Peptides 23 2002 1025 1029
21. H.X. Wang, and T.B. Ng Isolation of lilin, a novel arginine- and glutamate-rich protein with potent antifungal and mitogenic activities from lily bulbs Life Sci. 70 2002 1075 1084
22. K.T. Chu, and T.B. Ng First report of a glutamine-rich antifungal peptide with immunomodulatory and antiproliferative activities from family Amaryllidaceae Biochem. Biophys. Res. Commun. 325 2004 167 173
23. U.K. Laemmli, and M. Favre Gel electrophoresis of proteins J. Mol. Biol. 80 1973 573 599
24. S.S. Lam, H.X. Wang, and T.B. Ng Purification and characterization of novel ribosome inactivating proteins, alpha- and beta-pisavins from seeds of the garden pea Pisum sativum Biochem. Biophys. Res. Commun. 253 1998 135 142
25. T.B. Ng, and X.Y. Ye Fabin, a novel calcyon-like and glucanase-like protein with mitogenic, antifungal and translation-inhibitory activities from broad beans Biol. Chem. 384 2003 811 815
26. P.H.K. Ngai, and T.B. Ng Lentin, a novel and potent antifungal protein from shitake mushroom with inhibitory effects on activity of human immunodeficiency virus-1 reverse transcriptase and proliferation of leukemia cells Life Sci. 73 2003 3363 3374
27. X.Y. Ye, and T.B. Ng Isolation of a new cyclophilin-like protein from chickpeas with mitogenic, antifungal and anti-HIV-1 reverse transcriptase activities Life Sci. 70 2002 1129 1138
28. J.H. Wong, and T.B. Ng Purification of a trypsin stable lectin with antiproliferative and HIV-1 reverse transcriptase inhibitory activity Biochem. Biophys. Res. Commun. 301 2003 545 550
29. J.H. Wong, and T.B. Ng Isolation and characterization of a glucose/mannose/rhamnose-specific lectin from the knife bean Arch. Biochem. Biophys. 439 2005 91 98
30. P.H. Ngai, and T.B. Ng A mushroom (Ganoderma capense) lectin with spectacular thermostability, potent mitogenic activity on splenocytes, and antiproliferative activity toward tumor cell Biochem. Biophys. Res. Commun. 314 2004 988 993
31. C.H. Han, Q.H. Liu, T.B. Ng, and H.X. Wang A novel homodimeric lactose-binding lectin from the edible split gill medicinal mushroom Schizophyllum commune Biochem. Biophys. Res. Commun. 336 2005 252 257
32. H.X. Wang, and T.B. Ng First report of an arabinose-specific fungal lectin Biochem. Biophys. Res. Commun. 337 2005 621 625
33. T.B. Ng, Y.W. Lam, and H.X. Wang Calcaelin, a new protein with translation-inhibiting, antiproliferative and antimitogenic activities from the mosaic puffball mushroom Calvatia caelata Planta Med. 69 2003 212 217
34. S.K. Lam, and T.B. Ng First stimultaneous isolation of a ribosome inactivating protein and an antifungal protein from a mushroom (Lyophyllum shimeiji) together with evidence for synergism of their antifungal effects Arch. Biochem. Biophys. 393 2001 271 280
35. S.K. Lam, and T.B. Ng Hypsin, a novel thermostable ribosome-inactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom Hypsizigus marmoreus Biochem. Biophys. Res. Commun. 285 2001 1071 1075
36. H.X. Wang, and T.B. Ng Isolation and characterization of velutin, a novel low-molecular-weight ribosome inactivating protein from winter mushroom (Flammulina velutipes) fruiting bodies Life Sci. 68 2001 2151 2158
37. J.H. Wong, and T.B. Ng Sesquin, a potent defensin-like antimicrobial peptide from ground beans with inhibitory activities toward tumor cells and HIV-1 reverse transcriptase Peptides 26 2005 1120 1126
38. J.H. Wong, and T.B. Ng Vulgarinin, a broad-spectrum antifungal peptide from haricot beans (Phaseolus vulgaris) Int. J. Biochem. Cell Biol. 37 2005 1626 1632
39. P.H. Ngai, and T.B. Ng Phaseococcin, an antifungal protein with antiproliferative and anti-HIV-1 reverse transcriptase activities from small scarlet runner beans Biochem. Cell Biol. 83 2005 212 220
40. K.T. Chu, and T.B. Ng Purification and characterization of a chitinase-like antifungal protein from black turtle bean with stimulatory effect on nitric oxide production by macrophages Biol. Chem. 386 2005 19 24
41. L. Xia, and T.B. Ng Isolation of alliumin, a novel protein with antimicrobial and antiproliferative activities from multiple-cloved garlic bulbs Peptides 26 2005 177 183
42. P.H. Ngai, and T.B. Ng Coccinin, an antifungal peptide with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from large scarlet runner beans Peptides 25 2004 2063 2068
43. T.B. Ng, S.K. Lam, and W.P. Fong A homodimeric sporamin-type trypsin inhibitor with antiproliferative, HIV reverse transcriptase inhibitory and antifungal activities from wampee (Clausena lansium) seeds Biol. Chem. 384 2003 289 293
44. X.Y. Ye, T.B. Ng, and P.F. Rao A Bowman-Birk-type trypsin-chymotrypsin inhibitor from broad beans Biochem. Biophys. Res. Commun. 289 2001 91 96
45. X.Y. Ye, and T.B. Ng A new peptidic protease inhibitor from Vicia faba seeds exhibits antifungal, HIV-1 reverse transcriptase inhibiting and mitogenic activities J. Pept. Sci. 8 2002 656 662
46. P.H. Ngai, and T.B. Ng A napin-like polypeptide with translation- inhibitory, trypsin-inhibitory, antiproliferative and antibacterial activities from kale seeds J. Pept. Res. 64 2004 202 208
47. P.H. Ngai, and T.B. Ng A napin-like polypeptide from dwarf Chinese white cabbage seeds with translation-inhibitory, trypsin-inhibitory, and antibacterial activities Peptides 25 2004 171 176
48. M.V. Kolomiets, H. Chen, R.J. Gladon, E.J. Braun, and D.J. Hannapel A leaf lipoxygenase of potato induced specifically by pathogen infection Plant Physiol. 124 2000 1121 1130
49. M.V. Kolomiets, D.J. Hannapel, H. Chen, M. Tymeson, and R.J. Gladon Lipoxygenase is involved in the control of potato tuber development Plant Cell 13 2001 613 626
50. U. Maciejewska, L. Polkowaka-Kowalczyk, E. Swiezewska, and A. Szkopinska Plastoquinone: possible involvement in plant disease resistance Acta Biochim. Pol. 49 2002 775 780
51. T.B. Ng, W.K. Liu, S.F. Sze, and H.W. Yeung Action of alpha-momorcharin, a ribosome inactivating protein, on cultured tumor cell lines Gen. Pharmacol. 25 1994 1611 1616