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Volumn 348, Issue 1, 2006, Pages 154-156

Practical conditions for photoaffinity labeling with 3-trifluoromethyl-3- phenyldiazirine photophore

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

EID: 29144483964     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.10.015     Document Type: Article
Times cited : (22)

References (12)
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    • 3-Trifluoromethyl-3-phenyldiazirine: A new carbene generating group for photolabeling reagents
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    • T. Hosoya, T. Hiramatsu, T. Ikemoto, M. Nakanishi, H. Aoyama, A. Hosoya, T. Iwata, K. Maruyama, M. Endo, and M. Suzuki Novel bifunctional probe for radioisotope-free photoaffinity labeling: compact structure comprised of photospecific ligand ligation and detectable tag anchoring units Org. Biomol. Chem. 2 2004 637 641
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    • note
    • The stoichiometric amounts of photoligands to target molecules were used and the concentration of ligand was made to be 10 times higher than the binding constants of corresponding ligands for minimizing the unbound photoligand.


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