Determination of the viability of Aeromonas hydrophila in different types of water by flow cytometry, and comparison with classical methods

Applied and Environmental Microbiology

Volumn 71, Issue 12, 2005, Pages 7948-7954

  Pianetti, Anna ^a   Falcioni, Tania ^a   Bruscolini, Francesca ^a   Sabatini, Luigia ^a   Sisti, Elivio ^b   Papa, Stefano ^a  

^a UNIVERSITY OF URBINO   (Italy)

^b Center for Environmental Study   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

HEALTH; MICROBIOLOGY; PHYSIOLOGY; POTABLE WATER; RISK ASSESSMENT; RIVERS; SPECTROPHOTOMETRY;

AEROMONAS HYDROPHILA; FLOW CYTOMETRY; RIVER WATER; SPRING WATER;

MICROORGANISMS;

DNA; DRINKING WATER; MINERAL WATER; RIVER WATER; RNA;

BACTERIUM; DRINKING WATER; HEALTH RISK; VIABILITY;

AEROMONAS HYDROPHILA; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; FLOW CYTOMETRY; INTERMETHOD COMPARISON; MICROBIOLOGICAL EXAMINATION; NONHUMAN; SURVIVAL; WATER SAMPLING;

AEROMONAS HYDROPHILA; BACTERIOLOGICAL TECHNIQUES; CULTURE MEDIA; FLOW CYTOMETRY; HYDROGEN-ION CONCENTRATION; KINETICS; TIME FACTORS; WATER MICROBIOLOGY;

AEROMONAS; AEROMONAS HYDROPHILA; BACTERIA (MICROORGANISMS);

EID: 29144478863     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.12.7948-7954.2005     Document Type: Article

References (50)

1. Agenzia per la Protezione dell'Ambiente e per i Servizi Tecnici (APAT), Istituto di Ricerca sulle Acque del Consiglio Nazionale delle Ricerche (IRSA-CNR). 2003. Manuali e linee guida 29/2003. Agenzia per la Protezione dell'Ambiente e per i Servizi Tecnici (APAT), Istituto di Ricerca sulle Acque del Consiglio Nazionale delle Ricerche (IRSA-CNR), Rome, Italy.
2. Alvarez-Barrientos, A. M., J. Arroyo, R. Cantón, C. Nombela, and M. Sánchez-Pérez. 2000. Applications of flow cytometry to clinical microbiology. Clin. Microbiol. Rev. 13:167-195.
3. Araujo, R. M., R. M. Arribas, F. Lucena, and R. Pares. 1989. Relation between Aeromonas and faecal coliforms in fresh waters. J. Appl. Bacteriol. 76:213-217.
4. Ashbolt, N. J., A. Ball, M. Dorsch, C. Turner, P. Cox, A. Chapman, and S. M. Kirov. 1995. The identification and human health significance of environmental aeromonads. Water Sci. Technol. 31:263-269.
5. Barbesti, S., S. Citterio, M. Labra, M. D. Baroni, M. G. Neri, and S. Sgorbati. 2000. Two- and three-color fluorescence flow cytometric analysis of immunoidentified viable bacteria. Cytometry 40:214-218.
6. Biscardi, D., A. Castaldo, O. Gualillo, and R. de Fusco. 2002. The occurrence of cytotoxic Aeromonas hydrophila strains in Italian mineral and thermal waters. Sci. Total Environ. 292:255-263.
7. Borrel, N., M. J. Figueras, and J. Guarro. 1998. Phenotypic identification of Aeromonas genospecies from clinical and environmental sources. Can. J. Microbiol. 44:103-108.
8. Bouix, M., and J.-Y. Leveau. 2001. The applications of flow cytometry in microbiology. Bull. Soc. Fr. Microbiol. 16:210-218.
9. Boussaid, A., B. Baleux, L. Hassani, and J. Lesne. 1991. Aeromonas species in stabilization ponds in the arid region of Marrakesh, Morocco, and relation to fecal pollution and climatic factors. Microb. Ecol. 21:11-20.
10. Dalgaard, P., T. Ross, L. Kamperman, K. Neumeyer, and T. A. McMeekin. 1994. Estimation of bacterial growth rates from turbidimetric and viable count data. Int. J. Food Microbiol. 23:391-404.
11. Dalgaard, P., and K. Koutsoumanis. 2001. Comparison of maximum specific growth rates and lag times estimated from absorbance and viable count data by different mathematical models. J. Microbiol. Methods 43:183-196.
12. Davey, H. M., and D. B. Kell. 1996. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analysis. Microbiol. Rev. 60:641-696.
13. Davey, H. M., D. H. Welchart, D. B. Douglas, and A. S. Kaprelyants. 1999. Estimation of microbial viability using flow cytometry, p. 11.3.11-11.3.20. In Current protocols in cytometry. Wiley, New York, N.Y.
14. Farmer, J. J., III, M. J. Arduino, and F. W. Hickman-Brenner. 1992. The genera Aeromonas and Plesiomonas p. 3012-3028. In A. Balows, H. G. Trüper, M. Dworkin, W. Harder, and K.-H. Schleifer (ed.), The procaryotes, 2nd ed. Springer-Verlag, New York, N.Y.
15. Fiorentini, C., E. Barbieri, L. Falzano, P. Matarrese, W. Baffone, A. Pianetti, M. Katouli, I. Kühn, R. Möllby, F. Bruscolini, A. Casiere, and G. Donelli. 1998. Occurrence, diversity and pathogenicity of mesophilic Aeromonas in estuarine waters of the Italian coast of the Adriatic sea. J. Appl. Microbiol. 85:501-511.
16. Graviel, A. A., J. P. Landre, and A. J. Lamb. 1998. Incidence of mesophilic Aeromonas within a public drinking water supply in north-east Scotland. J. Appl. Microbiol. 84:383-392.
17. Hanninen, M. L., and A. Siitonen. 1995. Distribution of Aeromonas phenospecies among strains isolated from water, foods or from human clinical samples. Epidemiol. Infect. 115:39-50.
18. Haugland, R. P. 1998. Handbook of fluorescent probes and research chemicals, p. 680. Molecular Probes, Inc., Eugene, Oreg.
19. Havelaar, A. H., F. M. Schets, A. van Silfhout, W. H. Jansen, G. Wieten, and D. van der Kooij. 1992. Typing of Aeromonas strains from patients with diarrhoea and from drinking-water. J. Appl. Bacteriol. 72:435-444.
20. Ivanova, E. P., N. V. Zhukova, N. M. Gorshkova, and E. L. Chaikina. 2001. Characterization of Aeromonas and Vibrio species isolated from a drinking water reservoir. J. Appl. Microbiol. 90:919-927.
21. Janda, J. M., and S. L. Abbott. 1996. Human pathogens, p. 151-173. In B. Austin et al. (ed.), The genus Aeromonas. Wiley, London, United Kingdom.
22. Kaouther, B. A., P. Breeuwer, P. Verbaarschot, F. M. Rombouts, A. D. L. Akkermans, W. M. De Vos, and T. Abee. 2002. Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead Bifidobacterium cells during bile salt stress. Appl. Environ. Microbiol. 68:5209-5216.
23. Kirov, S. M. 1997. Aeromonas and Plesiomonas species, p. 265-287. In M. P. Doyle, L. R. Beuchat, and T. J. Montville (ed.), Food microbiology. Fundamentals and frontiers. AMS Press, Washington, D.C
24. Krovacek, K., V. Pasquale, S. B. Baloda, V. Soprano, M. Conte, and S. Dumontet. 1994. Comparison of putative virulence factors in Aeromonas hydrophila strains isolated from the marine environment and human diarrheal cases in southern Italy. Appl. Environ. Microbiol. 60:1379-1382.
25. Lebaron, P., N. Parthuisot, and P. Catala. 1998. Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems. Appl. Environ. Microbiol. 64:1725-1730.
26. Leclerc, H., and A. Moreau. 2002. Microbial safety of natural mineral water. FEMS Microbiol. Rev. 26:207-222.
27. Maalej, S., M. Denis, and S. Dukan. 2004. Temperature and growth-phase effects on Aeromonas hydrophila survival in natural seawater microcosms: role of protein synthesis and nucleic acid content on viable but temporarily nonculturable response. Microbiology 150:181-187.
28. Mary, P., N. E. Chihib, O. Charafeddine, C. Defives, and J. P. Hornez. 2002. Starvation survival and viable but noneulturable states in Aeromonas hydrophila. Microb. Ecol. 43:250-258.
29. Massa, S., C. Altieri, and A. D'Angela. 2001. The occurrence of Aeromonas spp. in mineral water and well water. Int. J. Food Microbiol. 63:169-173.
30. Merino, S., X. Rubiers, S. Knøchel, and J. M. Tornas. 1995. Emerging pathogens: Aeromonas spp. Int. J. Food Microbiol. 28:157-168.
31. Messi, P., E. Guerrieri, and M. Bondi. 2002. Survival of an Aeromonas hydrophila in an artificial mineral water microcosm. Water Res. 36:3410-3415.
32. Miettinen, I. T., T. Vartiainen, and P. J. Martikainen. 1997. Phosphorus and bacterial growth in drinking water. Appl. Environ. Microbiol. 63:3242-3245.
33. Morita, R. Y. 1993. Bioavailability of energy and the starvation state, p. 1-23. In S. Kjellerberg (ed.), Starvation in bacteria. Plenum Press, New York, N.Y.
34. Nebe-von-Caron, G., P. J. Stephens, C. J. Hewitt, J. R. Powell, and R. A. Badley. 2000. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J. Microbiol. Methods 42:97-114.
35. Oliver, J. D. 1993. Formation of viable but nonculturable cells, p. 239-272. In S. Kjellerberg (ed.), Starvation in bacteria. Plenum Press, New York, N.Y.
36. Oliver, J. D. 2000. The public health significance of viable but nonculturable bacteria, p. 277-300. In R. R. Colwell and D. J. Grimes (ed.), Nonculturable microrganisms in the environment. ASM Press, Washington, DC.
37. Pianetti, A., W. Baffone, F. Bruscolini, E. Barbieri, M. R. Biffi, L. Salvaggio, and A. Albano. 1998. Presence of several pathogenic bacteria in the Metauro and Foglia rivers (Pesaro-Urbino, Italy). Water Res. 32:1515-1521.
38. Pianetti, A., L. Sabatini, F. Bruscolini, F. Chiaverini, and G. Cecchetti. 2004. Faecal contamination indicators, Salmonella, Vibrio and Aeromonas in water used for the irrigation of agricultural products. Epidemiol. Infect. 132:231-238.
39. Porter, J., D. Deere, R. Pickup, and C. Edwards. 1996. Fluorescent probes and flow cytometry: new insights into environmental bacteriology. Cytometry 23:91-96.
40. Roszak, D. B., and R. R. Colwell. 1987. Survival strategies of bacteria in the natural environment. Microbiol. Rev. 51:365-379.
41. Sgorbati, S., S. Barbesti, M. G. Neri, C. Davegna, and S. Citterio. 1997. Rapid flow cytometric immunodetection of bacteria to monitor aquatic environments. Eur. J. Histochem. 41:187-188.
42. Shapiro, H. M. 2000. Microbial analysis at the single-cell level: tasks and techniques. J. Microbiol. Methods 42:3-16.
43. Soler, L., M. J. Figueras, M. R. Chacon, J. Vila, F. Marco, A. J. Martinez-Murcia, and J. Guarro. 2002. Potential virulence and antimicrobial susceptibility of Aeromonas popoffii recovered from freshwater and seawater. FEMS Immunol. Med. Microbiol. 32:243-247.
44. Thorne, S. H., and H. D. Williams. 1999. Cell density-dependent starvation survival of Rhizobium leguminosarum bv. phaseoli: identification of the role of an N-acyl homoserine lactone in adaptation to stationary-phase survival. J. Bacteriol. 181:981-990.
45. Thornley, J. P., J. G. Shaw, I. A. Gryllos, and A. Eley. 1996. Adherence of Aeromonas caviae to human cell lines Hep-2 and Caco-2. J. Med. Microbiol. 45:445-451.
46. Tsai, G. J., and S. C. Yu. 1997. Microbiological evaluation of bottled uncarbonated mineral water in Taiwan. Int. J. Food Microbiol. 37:137-143.
47. Vives-Riego, J., P. Lebaron, and G. Nebe-von-Caron. 2000. Current and future applications of flow cytometry in aquatic microbiology. FEMS Microbiol. Rev. 24:429-448.
48. Wadstrom, T., and A. Ljungh. 1991. Aeromonas and Plesiomonas as food and waterborne pathogens. Int. J. Food Microbiol. 12:303-312.
49. Wai, S. N., Y. Mizunoe, and A. Takade. 2000. A comparison of solid and liquid media for resuscitation of starvation- and low-temperature-induced nonculturable cells of Aeromonas hydrophila. Arch. Microbiol. 173:307-310.
50. Warburton, D. W., J. K. McCormick, and B. Bowen. 1994. Survival and recovery of Aeromonas hydrophila in water: development of methodology for testing bottled water in Canada. Can. J. Microbiol. 40:145-148.