Real-time pneumolysin polymerase chain reaction with melting curve analysis differentiates pneumococcus from other α-hemolytic streptococci

Diagnostic Microbiology and Infectious Disease

Volumn 53, Issue 4, 2005, Pages 293-299

  Kaijalainen, Tarja ^a   Saukkoriipi, Annika ^a   Bloigu, Aini ^a   Herva, Elja ^a   Leinonen, Maija ^a  

^a NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

Author keywords

hemolytic streptococcus; Nasopharyngeal specimen; Real time PCR; Streptococcus pneumoniae

Indexed keywords

NUCLEIC ACID; PNEUMOLYSIN;

ALPHA HEMOLYTIC STREPTOCOCCUS; ARTICLE; BACTERIUM ISOLATION; CONTROLLED STUDY; NASOPHARYNX; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; STREPTOCOCCUS PNEUMONIAE;

BACTERIAL PROTEINS; BASE SEQUENCE; DNA, BACTERIAL; HUMANS; INFANT; MOLECULAR SEQUENCE DATA; NASOPHARYNX; PNEUMOCOCCAL INFECTIONS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE; STREPTOLYSINS; TRANSITION TEMPERATURE;

EID: 29144476761     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2005.07.005     Document Type: Article

References (15)

1. J.C. Arbique, C. Poyart, P. Trieu-Cout, G. Quesne, M. da G.S. Carvalho, A.G. Steigerwalt, R.E. Morey, D. Jackson, R.J. Davidson, and R.R. Facklam Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. Nov J. Clin. Microbiol. 42 2004 4686 4696
2. D.G. Cvitkovitch, Y.H. Li, and R.P. Ellen Quorum sensing and biofilm formation in streptococcal infections J. Clin. Invest. 112 2003 1626 1632
3. G. Denys, and R.B. Carey Identification of Streptococcus pneumoniae with a DNA probe J. Clin. Microbiol. 30 1992 2725 2727
4. T. Kaijalainen, S. Rintamäki, E. Herva, and M. Leinonen Evaluation of gene-technological and conventional methods in the identification of Streptococcus pneumoniae J. Microbiol. Methods 51 2002 111 118
5. T. Kilpi, E. Herva, T. Kaijalainen, R. Syrjänen, and A.K. Takala Bacteriology of acute otitis media in a cohort of Finnish children followed for the first two years of life Pediatr. Infect. Dis. J. 20 2001 654 662
6. S. Kontiainen, and A. Sivonen Optochin resistance in Streptococcus pneumoniae strains isolated from blood and middle ear fluid Eur. J. Clin. Microbiol. 6 1987 422 424
7. L.S. Mundy, E.N. Janoff, K.E. Schwebke, C.J. Shanholtzer, and K.E. Willard Ambiguity in the identification of Streptococcus pneumoniae. Optochin, bile solubility, quellung, and the AccuProbe DNA Probe tests Am. J. Clin. Pathol. 109 1998 55 61
8. C. Neeleman, C.H.W. Klaassen, D.M. Klomberg, H.A. De Valk, and J.W. Mouton Pneumolysin is a key in misidentification of macrolide-resistant Streptococcus pneumoniae and is a putative virulence factor of S. mitis and other streptococci J. Clin. Microbiol. 42 2004 355 4357
9. S. Rintamäki, A. Saukkoriipi, P. Salo, A. Takala, and M. Leinonen Detection of Streptococcus pneumoniae DNA by using the polymerase chain reaction and microwell hybridisation with europium-labelled probes J. Microbiol. Methods 50 2002 313 318
10. Saukkoriipi A (2003) Ph.D. thesis. University of Oulu, Finland. Detection of pneumococcus by PCR.
11. A. Saukkoriipi, A. Palmu, T. Kilpi, and M. Leinonen Real-time quantitative PCR for detection of Streptococcus pneumoniae in the middle ear fluid of children with acute otitis media Mol. Cell. Probes 16 2002 385 390
12. P. Ukkonen, K. Varis, M. Jernfors, E. Herva, J. Jokinen, E. Ruokokoski, D. Zopf, and T. Kilpi Treatment of acute otitis media with an antiadhesive oligosaccharide: A randomised, double-blind, placebo-controlled trial Lancet 356 2000 1398 1402
13. R. Verhelst, T. Kaijalainen, T. De Baere, G. Verschraegen, G. Claeys, L. Van Simaey, C. De Ganck, and M. Vaheechoutte Comparison of five genotypic techniques for identification of optochin-resistant pneumococcus-like isolates J. Clin. Microbiol. 41 2003 3521 3525
14. A. Virolainen, P. Salo, J. Jero, P. Karma, J. Eskola, and M. Leinonen Comparison of PCR assay with bacterial culture for detecting Streptococcus pneumoniae in middle ear fluids of children with acute otitis media J. Clin. Microbiol. 32 1994 2667 2670
15. A.M. Whatmore, A. Efstratiou, A.P. Pickerill, K. Broughton, G. Woodard, D. Sturgeon, R. George, and C.G. Dowson Genetic relationships between clinical isolates of Streptococcus pneumoniae, Streptococcus oralis, and Streptococcus mitis: Characterization of "atypical" pneumococci and organisms allied to S. mitis harboring S. pneumoniae virulence factor-encoding genes Infect. Immun. 68 2000 1374 1382