Construction of engineered bifunctional enzymes and their overproduction in Aspergillus niger for improved enzymatic tools to degrade agricultural by-products

Applied and Environmental Microbiology

Volumn 71, Issue 12, 2005, Pages 8132-8140

  Levasseur, Anthony ^a,d   Navarro, David ^a   Punt, Peter J ^b   Belaïch, Jean Pierre ^a,c   Asther, Marcel ^a   Record, Eric ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b Quality of Life ^*  (Netherlands)

^c CNRS   (France)

^d Centre International de Ressources Microbiennes Champignons Filamenteux   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AGRICULTURE; BIODEGRADATION; BYPRODUCTS; CELLULOSE; IMMUNOLOGY; MICROBIOLOGY;

ASPERGILLUS NIGER; BIFUNCTIONAL ENZYMES; CHIMERIC ENZYMES; IMMUNODETECTION;

ENZYMES;

BINDING PROTEIN; C TERMINAL CARBOHYDRATE BINDING MODULE 1; ENDOXYLANASE B; ESTERASE; FERULIC ACID; FERULOYL ESTERASE A; HYBRID PROTEIN; HYDROLASE; MICROCRYSTALLINE CELLULOSE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE;

BIOTECHNOLOGY;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIGER; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CATALYST; CORN; ENZYME ACTIVITY; ENZYME ENGINEERING; IMMUNODETECTION; MICROBIAL DEGRADATION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYSICAL CHEMISTRY; PROTEIN BINDING; WHEAT BRAN;

AGRICULTURE; AMINO ACID SEQUENCE; ASPERGILLUS NIGER; BIODEGRADATION, ENVIRONMENTAL; CARBOXYLIC ESTER HYDROLASES; COUMARIC ACIDS; ENDO-1,4-BETA XYLANASES; GENE AMPLIFICATION; GENETIC VECTORS; KINETICS; MOLECULAR SEQUENCE DATA; MUTANT CHIMERIC PROTEINS; PROTEIN ENGINEERING;

AGRICULTURE; BIODEGRADATION; BYPRODUCTS; CELLULOSE; ENZYMES; MICROBIOLOGY;

ASPERGILLUS NIGER; TRITICUM AESTIVUM; ZEA MAYS;

EID: 29144464941     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.12.8132-8140.2005     Document Type: Article

References (49)

1. Bailey, M. J., P. Biely, and K. Poutanen. 1992. Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 23:257-270.
2. Bartolome, B., C. B. Faulds, P. A. Kroon, K. Waldron, H. J. Gilbert, G. Hazlewood, and G. Williamson. 1997. An Aspergillus niger esterase (ferulic acid esterase III) and a recombinant Pseudomonas fluorescens subsp. cellulosa esterase (Xy1D) release a 5-5′ ferulic dehydrodimer (diferulic acid) from barley and wheat cell walls. Appl. Environ. Microbiol. 63:208-212.
3. Béguin, P. 1999. Hybrid enzymes. Curr. Opin. Biotechnol. 10:336-340.
4. Bonnin, E., L. Lesage-Meesen, C. Stentelaire, M. Asther, and J. F. Thibault. 1999. Method for obtaining A. niger cultures and their uses for producing ferulic acid and vanillic acid. Patent 99/04644.
5. Bonnin, E., L. Saulnier, M. Brunel, C. Marot, L. Lesage-Meessen, M. Asther, and J. F. Thibault. 2002. Release of ferulic acid from agroindustrial by-products by the cell wall-degrading enzymes produced by Aspergillus niger I-1472. Enzyme Microb. Technol. 31:1000-1005.
6. Bunzel, M., J. Ralph, J. M. Marita, R. D. Hatfield, and H. Steinhart. 2001. Diferulates as structural components in soluble and insoluble cereal dietary fibre. J. Sci. Food Agric. 81:653-660.
7. Chanliaud, E., L. Saulnier, and J. F. Thibault. 1995. Alkaline extraction and characterisation of heteroxylans from maize bran. J. Cereal Sci. 21:195-203.
8. Conesa, A., P. J. Punt, N. van Luijk, and C. A. M. J. J. van den Hondel. 2001. The secretion pathway in filamentous fungi: a biotechnological view. Fungal Genet. Biol. 33:155-171.
9. de Vries, R., H. C. M. Kester, C. H. Poulsen, J. A. E. Benen, and J. Visser. 2000. Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides. Carbohydr. Res. 327:401-410.
10. de Vries, R. P., B. Michelsen, C. H. Poulsen., P. A. Kroon, R. H. H. van den Heuvel, C. B. Faulds, G. Williamson, J. P. T. W. van den Hombergh, and J. Visser. 1997. The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides. Appl. Environ. Microbiol. 63:4638-4644.
11. de Vries, R. P., P. A. van Kuyk, H. C. Kester, and J. Visser. 2002. The Aspergillus niger faeB gene encodes a second feruloyl esterase involved in pectin and xylan degradation and is specifically induced in the presence of aromatic compounds. Biochem. J. 363:377-386.
12. de Vries, R. P., and J. Visser. 2001. Aspergillus enzymes involved in degradation of plant cell wall polysaccharides. Microbiol. Mol. Biol. Rev. 65:497-522.
13. Din, N., Damude, H. G., N. R. Gilkes, R. C. Miller, Jr., R. A. Warren, and D. G. Kilburn. 1994. Cl-Cx revisited: intramolecular synergism in a cellulase. Proc. Natl. Acad. Sci. USA 91:11383-11387.
14. Din, N., N. R. Gilkes, B. Tekant, R. C., Jr. Miller, R. A. J. Warren, and D. G. Kilburn. 1991. Non-hydrolytic disruption of cellulose fibers by the binding domain of a bacterial cellulose. Biotechnology 9:1096-1099.
15. Faulds, C. B., and G. Williamson. 1995. Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE-III) from Aspergillus niger. Appl. Microbiol. Biotechnol. 463:1082-1087.
16. Faulds, C. B., and G. Williamson. 1999. Effect of hydroxycinnamates and benzoates on the production of feruloyl esterases by Aspergillus niger. J. Sci. Food Agric. 79:450-452.
17. Fierobe, H. P., F. Mingardon, A. Mechaly, A. Belaich, M. T. Rincon, S. Pages, R. Lamed, C. Tardif, J. P. Belaich, and E. A. Bayer. 2005. Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin. J. Biol. Chem. 280:16325-16334.
18. Garcia-Conesa, M. T., V. F. Crepin, A. J. Goldson, G. Williamson, N. J. Cummings, I. F. Connerton, C. B. Faulds, and P. A. Kroon. 2004. The feruloyl esterase system of Talaromyces stipitatus: production of three discrete feruloyl esterases, including a novel enzyme, TsFaeC, with a broad substrate specificity. J. Biotechnol. 108:227-241.
19. Gasteiger, E., C. Hoogland, A. Gattiker, S. Duvaud, M. R. Wilkins, R. D. Appel, and A. Bairoch. 2005. Protein identification and analysis tools on the ExPASy server, p. 571-607. In J. M. Walker (ed.), The proteomics protocols handbook. Humana Press, Inc., Totowa, N.J.
20. Gielkens, M. M., E. Dekkers, J. Visser, and L. H. de Graaff. 1999. Two cellobiohydrolase-encoding genes from Aspergillus niger require D-xylose and the xylanolytic transcriptional activator XlnR for their expression. Appl. Environ. Microbiol. 65:4340-4345.
21. Goldstein, M. A., M. Takagi, S. Hashida, O. Shoseyov, R. H. Doi, and I. H. Segel. 1993. Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A. J. Bacteriol. 175:5762-5768.
22. Gordon, C. L., D. B. Archer, D. J. Jeenes, J. H. Doonan, B. Wells, A. P. J. Trinci, and G. D. Robson. 2000. A glucoamylase::GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger. J. Microbiol. Methods 42:39-48.
23. Graf, E. 1992. Antioxidant potential of ferulic acid. Free Radic. Biol. Med. 13:435-448.
24. Ho, S. N., H. D. Hunt, R. M. Horton, J. K. Pullen, and L. R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
25. Juge, N., G. Williamson, A. Puigserver, N. J. Cummings, I. F. Connerton, and C. B. Faulds. 2001. High-level production of recombinant Aspergillus niger cinnamoyl esterase (FAEA) in the methylotrophic yeast Pichia pastoris. FEMS Yeast Res. 1:127-132.
26. Kikuzaki, H., M. Hisamoto, K. Hirose, K. Akiyama, and H. Taniguchi. 2002. Antioxidant properties of ferulic acid and its related compounds. J. Agric. Food Chem. 50:2161-2168.
27. Kroon, P. A., G. Williamson, N. M. Fish, D. B. Archer, and N. J. Belshaw. 2000. A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module. Eur. J. Biochem. 267:6740-6752.
28. Ljungcrantz, P., H. Carlsson, M. O. Mansson, P. Buckel, K. Mosbach, and L. Bulow. 1989. Construction of an artificial bifunctional enzyme, beta-galactosidase/galactose dehydrogenase, exhibiting efficient galactose channeling. Biochemistry 28:8786-8792.
29. Levasseur, A., M. Asther, and E. Record. 2005. Overproduction and characterization of xylanase B from Aspergillus niger. Can. J. Microbiol. 51:177-183.
30. Levasseur, A., I. Benoit, M. Asther, M. Asther, and E. Record. 2004. Homologous expression of the feruloyl esterase B gene from Aspergillus niger and characterization of the recombinant enzyme. Protein Expr. Purif. 37:126-133.
31. Levasseur, A., S. Pagès, H. P. Fierobe, D. Navarro, P. J. Punt, J. P. Belaïch, M. Asther, and E. Record. 2004. Design and production in Aspergillus niger of a chimeric protein associating a fungal feruloyl esterase and a clostridial dockerin domain. Appl. Environ. Microbiol. 70:6984-6991.
32. Libby, C. B., C. A. Cornett, P. J. Reilly, and C. Ford. 1994. Effect of amino acid deletions in the O-glycosylated region of Aspergillus awamori glucoamylase. Protein Eng. 7:1109-1114.
33. Linder, M., and T. T. Teeri. 1997. The roles and function of cellulose-binding domains. J. Biotechnol. 57:15-28.
34. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31:426-428.
35. Nixon, A. E., M. Ostermeier, and S. J. Benkovic. 1998. Hybrid enzymes: manipulating enzyme design. Trends Microbiol. 16:258-264.
36. Pagès, S., L. Gal, A. Belaich, C. Gaudin, C. Tardif, and J. P. Belaich. 1997. Role of scaffolding protein CipC of Clostridium cellulolyticum in cellulose degradation. J. Bacteriol. 179:2810-2816.
37. Porath, J., J. Carlsson, I. Olsson, and G. Belfrage. 1975. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258:598-599.
38. Punt, P. J., and C. A. van den Hondel. 1992. Transformation of filamentous fungi based on hygromycin B and phleomycine resistance markers. Methods Enzymol. 216:447-457.
39. Ralet, M. C., C. B. Faulds, G. Williamson, and J. F. Thibault. 1994. Degradation of feruloylated oligosaccharides from sugar-beet pulp and wheat bran by ferulic acid esterases from Aspergillus niger. Carbohydr. Res. 263:257-269.
40. Record, E., M. Asther, C. Sigoillot, S. Pagès, P. J. Punt, M. Delattre, M. Haon, C. A. van den Hondel, J. C. Sigoillot, L. Lesage-Meessen, and M. Asther. 2003. Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application. Appl. Microbiol. Biotechnol. 62:349-355.
41. Sagt, C. M., B. Kleizen, R. Verwaal, M. D. deJong, W. H. Muller, A. Smits, C. Visser, J. Boonstra, A. J. Verkleij, and C. T. Verrips. 2000. Introduction of N glycosylation site increases secretion of heterologous proteins in yeasts. Appl. Environ. Microbiol. 66:4940-4944.
42. Saulnier, L., and J. F. Thibault. 1999. Ferulic acid and diferulic acids as components of sugar-beet pectins and maize bran heteroxylans. J. Sci. Food Agric. 79:396-402.
43. Shibuya, I., G. Tamura, H. Shima, T. Ishikawa, and S. Hara. 1992. Construction of an α-amylase/glucoamylase fusion gene and its expression in Saccharomyces cerevisiae. Biosci. Biotech. Biochem. 56:884-889.
44. Srisodsuk, M., J. Lehtio, M. Linder, E. Margolles-Clark, and T. Reinikainen, and T. T. Teeri. 1997. Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose. J. Biotechnol. 57:49-57.
45. Tomme, P. N., R. Gilkes, C. M. Miller, Jr., A. J. Warren, and D. G. Kilburn. 1994. An internal cellulose-binding domain mediates adsorption of an engineered bifunctional xylanase/cellulase. Protein Eng. 7:117-123.
46. Topakas, E., H. Stamatis, P. Biely, and P. Christakopoulos. 2004. Purification and characterization of a type B feruloyl esterase (StFAE-A) from the thermophilic fungus Sporotrichum thermophile. Appl. Microbiol. Biotechnol. 63:686-690.
47. van Hartingsveldt, W., I. E. Mattern, C. M. van Zeul, P. H. Pouwells, and C. A. van den Hondel. 1987. Development of a homologous transformation system for Aspergillus niger based on the pyrG gene. Mol. Gen. Genet. 206:71-75.
48. Wood, T. M. 1988. Preparation of crystalline, amorphous, and dyed cellulase substrates Methods Enzymol. 160:19-25.
49. Yu, P., D. D. Maenz, J. J. McKinnon, V. J. Racz, and D. A. Christensen. 2002. Release of ferulic acid from oat hulls by Aspergillus ferulic acid esterase and Trichoderma xylanase. J. Agric. Food Chem. 50:1625-1630.