Diversity of Acacia tortilis rhizobia revealed by PCR/RFLP on crushed root nodules in Tunisia

Annals of Microbiology

Volumn 55, Issue 4, 2005, Pages 249-254

  Ben Romdhane, Samir ^a,c,d   Nasr, Hafedh ^a   Samba Mbaye, Rama ^b   Neyra, Marc ^b   Ghorbal, Mohamed Habib ^c  

^a UNIVERSITY OF CARTHAGE   (Tunisia)

^b Centre IRD de Bel Air   (Senegal)

^c FACULTÉ DES SCIENCES DE TUNIS   (Tunisia)

^d Institut National de Recherche Scientifique et Technique Tunis   (Tunisia)

Author keywords

Acacia tortilis; Diversity; Nodules extracted DNAs; PCR RFLP; Tunisia

Indexed keywords

ACACIA; ACACIA TORTILIS; AGROBACTERIUM; MESORHIZOBIUM; RHIZOBIACEAE; RHIZOBIUM; SINORHIZOBIUM;

EID: 29144463607     PISSN: 15904261     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Ba S., Willems A., De Lajudie P., Roche P., Jeder H., Quatrini P., Neyra M., Ferro M., Prome J-C., Gillis M., Boivin-Masson C., Lorquin J. (2002). Symbiotic and taxonomic diversity of rhizobia isolated from Acacia tortilis subsp. raddiana in Africa. System. Appl. Microbiol., 25: 130-145.
2. Ba A.M., Samba R., Sylla S.N., Le Roux C., Neyra M., Rousteau A., Imbert D., Toribio A. (2004). Caractérisation de la diversité des micoorganismes symbiotiques de Pterocarpus officinalis dans des forêts marécageuses de Guadeloupe et Martinique. Revue d'Ecologie (Terre et Vie), 59: 163-170.
3. Benhizia Y., Benhizia H., Benguedouar A., Muresu R., Giacomini A., Squartini A. (2004). Gamma proteobacteria can nodulate legumes of the genus Hedysarum. System. Appl. Microbiol., 27: 462-468.
4. Chen L.S., Figueredo A., Pedrosa F.O., Hungria M. (2000). Genetic characterization of Soybean rhizobia in Paraguay. Appl. Environ. Microbiol., 66: 5099-5103.
5. Chen W.M., Moulin L., Bontemps C., Vandamme P., Bena G., Boivinmasson C. (2003). Legume symbiotic nitrogen fixation by beta-proteobacteria is widespread in nature. J. Bacteriol., 185: 7266-7272.
6. De Lajudie P., Willems A., Nick G., Mohamed S.H., Torck U., Coopman R., Filali-Maltouf A., Kersters K., Dreyfus B., Lindström K., Gillis M. (1999). Agrobacterium bv.1 strains isolated from nodules of tropical legumes. System. Appl. Microbiol., 22: 119-132.
7. Demba D.M. (1999). Optimisation d'un protocole d'extraction et de purification d'ADN de nodules de légumineuses. Mémoire de DEA, Université Cheikh Anta Diop, Dakar, Sénégal. pp. 55.
8. Demezas D.H., Reardon T.B., Strain S.R., Watson J.M., Gibson A.H. (1995). Diversity and genetic structure of a natural population of Rhizobium leguminosarum bv. trifolii subterraneum L. Mol. Ecol., 4: 209-220.
9. Geniaux E., Laguerre G., Amarger N. (1993). Comparison of geographically distant populations of Rhizobium isolated from root nodules of Phaseolus vulgaris. Mol. Ecol., 2: 295-302.
10. Gtari M., Brusetti L., Aouani M.E., Daffonchio D., Boudabous A. (2002). Frankia nodulating Alnus glutinosa and Casuarinaceae in Tunisia. Ann. Microbiol., 52: 145-153.
11. Gürtler V., Stanisich V.A. (1996). New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology, 142: 3-16.
12. Harasawa R., Mizusawa H., Nozawa K., Nakagawa T., Asada K., Kato I. (1993). Detection and tentative identification of dominant Mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regions. Res. Microbiol., 144: 489-493.
13. Huber I., Selenska-Pobell S. (1994). Pulsed-field electrophoresis- fingerprinting, genome size estimation and rrn loci number of Rhizobium galegae. J. Appl. Bacteriol., 77: 528-533.
14. Jensen M.A., Webster J., Straus N. (1993). Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl. Environ. Microbiol., 59: 945-952.
15. Khbaya B., Neyra M., Normand P., Zerhari K., Filali-Maltouf A. (1998) Genetic diversity and phylogeny of rhizobia that nodulate Acacia spp. in Morroco assessed by analysis of rRNA genes. Appl. Environ. Microbiol., 64: 4912-4917.
16. Laguerre G., Allard M.R., Revoy F., Amarger N. (1994) Rapid identification of rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes. Appl. Environ. Microbiol., 60: 56-63.
17. Mhamdi R., Laguerre G., Aouani M.E., Mars M., Amarger N. (2002). Different species and symbiotic genotypes of field rhizobia can nodulate Phaseolus vulgaris in Tunisian soils. FEMS Microbiol. Ecol., 41: 77-84.
18. nd edn. Faculté des Sciences, Tunis, Tunisie, pp. 132-133.
19. Navarro E., Simonet P., Normand P., Bardin R. (1992). Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer. Arch. Microbiol., 157: 107-115.
20. Odee D.W., Haukka K., McInroy S.G., Sprent J.I., Sutherland J.M., Young J.P.W. (2002). Genetic and symbiotic characterization of rhizobia isolated from tree and herbaceous legumes grown in soils from ecologically diverse sites in Kenya. Soil Biol. Biochem., 34: 804-811.
21. Ponsonnet C., Nesme X. (1994). Identification of Agrobacterium strains by PCR-RFLP analysis of pTi and chromosomal regions. Arch. Microbiol., 16: 300-309.
22. Rouvier C., Prin Y., Reddell P., Normand P., Simonet P. (1996). Genetic diversity among Frankia strains nodulating members of the family Casuarinaceae in Australia revealed by PCR and restriction fragment length polymorphism analysis with crushed root nodules. Appl. Environ. Microbiol., 62: 979-985.
23. Schofield P.R., Gibson A.H., Dudman W.F., Watson J.M. (1987). Evidence for genetic exchange and recombination of Rhizobium symbiotic plasmids in a soil population. Appl. Environ. Microbiol., 53: 2942-2947.
24. Thiao M., Neyra M., Isidore E., Sylla S., Lesueur D. (2004) Diversity and effectiveness of rhizobium from Gliricidia sepium native to Reunion Island, Kenya and New Caledonia. World J. Microbiol., 20: 703-709.
25. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. (1991). 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol., 173: 697-703.
26. Willems A. (2003). The taxonomy of rhizobia: an overview. Plant Soil, 1-11.
27. Zahran H.H. (2001). Rhizobia from wild legumes: diversity, taxonomy, ecology, nitrogen fixation and biotechnology. J. Biotech., 91: 143-153.