Characterisation of cytochrome c unfolding by nano-electrospray ionization time-of-flight and Fourier transform ion cyclotron resonance mass spectrometry

European Journal of Mass Spectrometry

Volumn 11, Issue 5, 2005, Pages 519-524

  Youhnovski, Nikolay ^a   Matečko, Irena ^b   Šamalikova, Maria ^b   Grandori, Rita ^b  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b JOHANNES KEPLER UNIVERSITY LINZ   (Austria)

Author keywords

Electrostatic effect; Folding intermediates; Hydrophobic effect; Protein folding; Secondary and tertiary protein structure

Indexed keywords

CYTOCHROME C; ION; TRIFLUOROETHANOL;

ANIMAL; CHEMISTRY; COMPARATIVE STUDY; CONFERENCE PAPER; CYCLOTRON; ELECTROSPRAY MASS SPECTROMETRY; FOURIER ANALYSIS; HORSE; IN VITRO STUDY; MASS SPECTROMETRY; METHODOLOGY; NANOTECHNOLOGY; PH; PROTEIN DENATURATION; PROTEIN FOLDING;

ANIMALS; CYCLOTRONS; CYTOCHROMES C; FOURIER ANALYSIS; HORSES; HYDROGEN-ION CONCENTRATION; IONS; MASS SPECTROMETRY; NANOTECHNOLOGY; PROTEIN DENATURATION; PROTEIN FOLDING; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRIFLUOROETHANOL;

EID: 29144442876     PISSN: 14690667     EISSN: None     Source Type: Journal    
DOI: 10.1255/ejms.730     Document Type: Conference Paper

References (24)

1. M. Przybylski and O. Glocker, "Electrospray mass spectrometry of biomacromolecule complexes with non-covalent interactions - new analytical perspectives for supramolecular chemistry and molecular recognition processes", Angew. Chem. Int. Ed. Engl. 35, 806 (1996).
2. R. Grandori, "Electrospray-ionization mass spectrometry for protein conformational studies", Curr. Org. Chem. 7, 1589 (2003).
3. S.K. Chowdhury, V. Katta and B.T. Chait, "Probing conformational changes in proteins by mass spectrometry", J. Am. Chem. Soc. 112, 9012 (1990).
4. M. Šamalikova, I. Matečko, N. Müller and R. Grandori, "Interpreting conformational effects in protein nano-ESI-MS spectra", Anal. Bioanal. Chem. 378, 1112 (2004).
5. R. Grandori, I. Matečko and N. Müller, "Uncoupled analysis of secondary and tertiary protein structure by circular dichroism and electrospray ionization mass spectrometry", J. Mass Spectrom. 37, 191 (2001).
6. X.M. Pan, X.R. Sheng and J.M. Zhou, "Probing subtle acid-induced conformational changes of ribonuclease A by electrospray mass spectrometry", FEBS Lett. 402, 25 (1997).
7. L. Konermann and D.J. Douglas, "Acid-induced unfolding of cytochrome c at different methanol concentrations: electrospray ionization mass spectrometry specifically monitors changes in the tertiary structure." Biochemistry 36, 12296 (1997).
8. J.W. Nelson and N.R. Kallenbach, "Stabilization of the ribonuclease S-peptide alpha-helix by trifluoroethanol", Proteins 1, 211 (1986).
9. A. Cammers-Goodwin, T.J. Allen, S.L. Oslick, K.F. McClure, J.H. Lee and D.S. Kemp, "Mechanism of stabilization of helical conformations of polypeptides by water containing trifluoroethanol", J. Am. Chem. Soc. 118, 3082 (1996).
10. E.R. Main and S.E. Jackson, "Does trifluoroethanol affect folding pathways and can it be used as a probe of structure in transition states?" Nature Struct. Biol. 6, 831 (1999).
11. R. Grandori, "Detecting equilibrium cytochrome c folding intermediates by electrospray ionization mass spectrometry: two partially folded forms populate the molten-globule state", Protein Sci. 11, 453 (2002).
12. T. Konno, J. Iwashita and K. Nagayama, "Fluorinated alcohol, the third group of cosolvents that stabilize the molten-globule state relative to a highly denatured state of cytochrome c", Protein Sci. 9, 564 (2000).
13. D. Hamada, F. Chiti, J.I. Guijarro, M. Kataoka, N. Taddei and C.M. Dobson, "Evidence concerning rate-limiting steps in protein folding from the effects of trifluoroethanol." Nature Struct. Biol. 7, 58 (2000).
14. L.C. Wu, P.B. Laub, G.A. Elove, J. Carey and H. Roder, "A noncovalent peptide complex as a model for an early folding intermediate of cytochrome c." Biochemistry 32, 10271 (1993).
15. T.A. Fligge, M. Przybylski, J.P. Quinn and A.G. Marshall, "Evaluation of heat-induced conformational changes in proteins by nanoelectrospray Fourier transform ion cyclotron resonance mass spectrometry", Eur. Mass Spectrom. 4, 401 (1998).
16. M. Fountoulakis, F. Vilbois, G. Oesterhelt and W. Vetter, "Phosphoric acid entrapment leads to apparent protein heterogeneity", Biotechnology 13, 383 (1995).
17. 2O", Biochemistry 32, 11878 (1993).
18. S.E. Rodriguez-Cruz, J.T. Khoury and J.H. Parks, "Protein fluorescence measurements within electrospray droplets", J. Am. Soc. Mass Spectrom. 12, 716 (2001).
19. M. Šamalikova and R. Grandori, "Protein charge state distributions in electrospray-ionization mass spectrometry do not appear to be limited by the surface tension of the solvent", J. Am. Chem. Soc. 125, 13352 (2003).
20. M. Šamalikova and R. Grandori, "Role of opposite charges in protein electrospray-ionization mass spectrometry", J. Mass Spectrom. 38, 941 (2003).
21. M. Šamalikova and R. Grandori, "Testing the role of solvent surface tension in protein ionization by electrospray", J. Mass Spectrom. 40, 503 (2005).
22. A.T. Iavarone and E.R. Williams, "Mechanism of charging and supercharging molecules in electrospray ionization", J. Am. Chem. Soc. 125, 2319 (2003).
23. J.F. de la Mora, "Electrospray ionization of large multiply charged species proceeds via Dole's charged residue mechanism", Anal. Chim. Acta 406, 93 (2000).
24. D.R. Lide, in CRC Handbook of Chemistry and Physics, 82nd Edn, Ed by D.R. Lide. CRC Press, Boca Raton, FL, USA (2001).