The effects of different decalcification protocols on TUNEL and general cartilage staining

Biotechnic and Histochemistry

Volumn 80, Issue 3-4, 2005, Pages 111-115

  Emans, P J ^a   Bulstra, S K ^a   Kuijer, R ^a,b  

^a MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Cartilage; Decalcification; EDTA; Proteinase K; TUNEL

Indexed keywords

ACETIC ACID; CELL DNA; CITRIC ACID; EDETIC ACID; PROTEINASE K; TRICHLOROACETIC ACID;

ANIMAL TISSUE; APOPTOSIS; ARTICLE; CARTILAGE CELL; CHEMICAL PROCEDURES; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME DEGRADATION; FEMALE; FEMUR CONDYLE; HISTOLOGY; IMMUNOHISTOCHEMISTRY; NICK END LABELING; NONHUMAN; RABBIT;

ACETIC ACID; ANIMALS; CARTILAGE, ARTICULAR; CITRIC ACID; DECALCIFICATION TECHNIQUE; EDETIC ACID; FEMALE; IN SITU NICK-END LABELING; RABBITS; SPECIMEN HANDLING; STAINING AND LABELING; TRICHLOROACETIC ACID;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 29044440015     PISSN: 10520295     EISSN: 14737760     Source Type: Journal    
DOI: 10.1080/10520290500159253     Document Type: Article

References (12)

1. Aigner, T Hemmel, M Neureiter, D Gebhard, PM Zeiler, G Kirchner, T McKenna, L. (2001) Apoptotic cell death is not a widespread phenomenon in normal aging and osteoarthritis human articular knee cartilage: A study of proliferation, programmed cell death (apoptosis), and viability of chondrocytes in normal and osteoarthritic human knee cartilage Arthritis Rheum., 44, pp. 1304 - 1312.
2. Alers, JC Krijtenburg, PJ Vissers, KJ van Dekken, H. (1999) Effect of bone decalcification procedures on DNA in situ hybridization and comparative genomic hybridization. EDTA is highly preferable to a routinely used acid decalcifier J. Histochem. Cytochem., 47, pp. 703 - 710.
3. Arber, J Weiss, L Chang, K Battifora, H Arber, D. (1997) The effect of decalcification on in situ hybridization Mod. Pathol., 10, pp. 1009 - 1014.
4. Blanco, FJ Guitian, R Vazquez-Martul, E de Toro, FJ Galdo, F. (1998) Osteoarthritis chondrocytes die by apoptosis. A possible pathway for osteoarthritis pathology Arthritis Rheum., 41, pp. 284 - 289.
5. Gavrieli, Y Sherman, Y Ben-Sasson, SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation J. Cell Biol., 119, pp. 493 - 501.
6. Hashimoto, S Koji, T Niu, J Kanematsu, T Nakane, PK. (1995) Differential staining of DNA strand breaks in dying cells by non-radioactive in situ nick translation Arch. Histol. Cytol., 58, pp. 161 - 170.
7. Hashimoto, S Ochs, RL Komiya, S Lotz, M. (1998) Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis Arthritis Rheum., 41, pp. 1632 - 1638.
8. Kaneko, H Ogiuchi, H Shimono, M. (1997) Cell death during tooth eruption in the rat: Surrounding tissues of the crown Anat. Embryol. (Berl)., 195, pp. 427 - 434.
9. Kim, HA Lee, YJ Seong, SC Choe, KW Song, YW. (2000) Apoptotic chondrocyte death in human osteoarthritis J Rheumatol., 27, pp. 455 - 462.
10. Kouri, JB Aguilera, JM Reyes, J Lozoya, KA Gonzalez, S. (2000) Apoptotic chondrocytes from osteoarthrotic human articular cartilage and abnormal calcification of subchondral bone J. Rheumatol., 27, pp. 1005 - 1019.
11. Sakai, T Kiyoshima, T Kobayashi, I Moroi, R Ibuki, T Nagadome, M Terada, Y Sakai, H. (1999) Age-dependent changes in the distribution of BrdU- and TUNEL-positive cells in the murine gingival tissue J. Periodontol., 70, pp. 973 - 981.
12. Yamamoto-Fukuda, T Shibata, Y Hishikawa, Y Shin, M Yamaguchi, A. (2000) Effects of various decalcification protocols on detection of DNA strand by terminal dUTP nick end labelling Histochem. J., 32, pp. 697 - 702.