Crystallization and preliminary X-ray crystallographic analysis of PAS factor from Vibrio vulnificus

Molecules and Cells

Volumn 20, Issue 3, 2005, Pages 361-363

  Lee, Jun Hyuck ^a   Kim, Soo Young ^b   Rho, Seong Hwan ^a   Im, Young Jun ^a   Kim, Young Ran ^b   Kim, Mun Kyoung ^a   Kang, Gil Bu ^a   Rhee, Joon Haeng ^b   Eom, Soo Hyun ^a,c  

^a Gwangju Institute of Science and Technology (GIST)   (South Korea)

^b Chonnam National University Medical School   (South Korea)

^c Gwangju TechnoPark   (South Korea)

Author keywords

PAS Factor; Secretion; Vibrio vulnificus; X Ray Crystallography

Indexed keywords

BACTERIAL PROTEIN; CRYOPROTECTIVE AGENT; MONOMER; PERIPLASMIC PROTEIN; PROTEIN PAS FACTOR; SODIUM BROMIDE; UNCLASSIFIED DRUG;

ACHROMOBACTER; ARTICLE; CONTROLLED STUDY; CRYSTALLIZATION; HANGING DROP VAPOR DIFFUSION METHOD; NONHUMAN; PLASMID; PROTEIN SECRETION; PURIFICATION; VIBRIO VULNIFICUS; X RAY CRYSTALLOGRAPHY; CHEMISTRY;

ACHROMOBACTER; BACTERIA (MICROORGANISMS); VIBRIO VULNIFICUS;

BACTERIAL PROTEINS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; VIBRIO VULNIFICUS;

EID: 28944433395     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Finlay, B. B. and Falkow, S. (1997) Common themes in microbial pathogenicity revisited. Microbiol. Mol. Biol. Rev. 61, 136-169.
2. Gray, L. D. and Kreger, A. S. (1989) Detection of Vibrio vulnificus cytolysin in V. vulnificus-infected mice. Toxicon 27, 439-464.
3. Hampel, A., Labanauskas, M., Connor, P. G., Kirkegard, L., RajBhandary, U. L., et al. (1968) Single crystals of transfer RNA from formylmethionine and phenylalanine transfer RNA's. Science 162, 1384-1387.
4. Jeong, M. S. and Jang, S. B. (2005) Crystallization and X-ray crystallographic studies of wild-type and mutant tryptophan synthase α-subunits from Escherichia coli. Mol. Cells 19, 219-222.
5. Kim, Y. R., Lee, S. E., Kim, C. M., Kim, S. Y., Shin, E. K., et al. (2003) Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients. Infect. Immun. 71, 5461-5471.
6. Kreger, A., DeChatelet, L., and Shirley, P. (1981) Interaction of Vibrio vulnificus with human polymorphonuclear leukocytes: association of virulence with resistance to phagocytosis. J. Infect. Dis. 144, 244-248.
7. Matthews, B. W. (1968) Solvent content of protein crystals. J. Mol. Biol. 33, 491-497.
8. McPherson, V. L., Watts, J. A., Simpson, L. M., and Oliver, J. D. (1991) Physiological effects of the lipopolysaccharide of Vibrio vulnificus on mice and rats. Microbios 67, 141-149.
9. Miroux, B. and Walker, J. E. (1996) Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260, 289-298.
10. Morris, J. G. Jr., Wright, A. C., Simpson, L. M., Wood, P. K., Johnson, D. E., et al. (1987) Virulence of Vibrio vulnificus: association with utilization of transferrin-bound iron, and lack of correlation with levels of cytotoxin or protease production. FEMS Microbiol. Lett. 40, 55-59.
11. Oliver, J. D., Wear, J. E., Thomas, M. B., Warner, M., and Linder, K. (1986) Production of extracellular enzymes and cytotoxicity by Vibrio vulnificus. Diagn. Microbiol. Infect. Dis. 5, 99-111.
12. Otwinowsky, Z. and Minor, W. (1997) Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307-326.
13. Simpson, L. M. and Oliver, J. D. (1987) Ability of Vibrio vulnificus to obtain iron from transferrin and other iron-binding proteins. Curr. Microbiol. 15, 155-157.
14. Terwilliger, T. C. (1999) Reciprocal-space solvent flattening. Acta Cryst. D55, 1863-1871.
15. Terwilliger, T. C. and Berendzen, J. (1999) Automated MAD and MIR structure solution. Acta Cryst. D55, 849-861.
16. Tokugawa, K., Kakitani, M., Ishii, T., Nakamura, K., Masaki, H., et al. (1994a) A novel protein secretion factor from a Vibrio species which operates in Escherichia coli. J. Biotechnol. 35, 69-76.
17. Tokugawa, K., Ishii, T., Nakamura, K., Masaki, H., and Uozumi, T. (1994b) A model system for the continuous production of a heterologous protein using a novel secretion promoting factor which operates in Escherichia coli. J. Biotechnol. 37, 33-37.
18. Wright, A. L., Miceli, G. A., Landry, W. L., Christy, J. B., Watkins, W. D., et al. (1993) Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe. Appl. Environ. Microbiol. 59, 541-546.