Characterization of a monolithic immobilized trypsin microreactor with on-line coupling to ESI-MS

Journal of Separation Science

Volumn 28, Issue 14, 2005, Pages 1675-1684

  Křenková, Jana ^a,b   Bilková, Zuzana ^b   Foret, František ^a  

^a INSTITUTE OF ANALYTICAL CHEMISTRY   (Czech Republic)

^b UNIVERSITY OF PARDUBICE   (Czech Republic)

Author keywords

Immobilized enzymatic reactor; Immobilized trypsin; Mass spectrometry; Monolithic column

Indexed keywords

BIOREACTORS; CHARACTERIZATION; MASS SPECTROMETERS; PH EFFECTS; THERMAL EFFECTS;

IMMOBILIZED ENZYMATIC REACTOR; IMMOBILIZED TRYPSIN; MONOLITHIC COLUMN; TRYPSIN MICROREACTOR;

ENZYME IMMOBILIZATION;

CYTOCHROME C; EPOXIDE; FUNCTIONAL GROUP; METHACRYLIC ACID DERIVATIVE; POLYMER; PROTEINASE; SILICON DIOXIDE; TOSYLPHENYLALANYL CHLOROMETHYL KETONE; TRYPSIN;

ARTICLE; BIOREACTOR; CAPILLARY CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; COVALENT BOND; ELECTROSPRAY MASS SPECTROMETRY; FLOW RATE; IMMOBILIZED ENZYME REACTOR; LABORATORY TEST; LIQUID; NONHUMAN; ONLINE ANALYSIS; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN IMMOBILIZATION; PROTEIN INTAKE; REACTION ANALYSIS; SEQUENCE ANALYSIS; STANDARDIZATION; TEMPERATURE MEASUREMENT; TIME OF FLIGHT MASS SPECTROMETRY;

CAPILLARITY; ENZYMES, IMMOBILIZED; INDICATORS AND REAGENTS; SILICON DIOXIDE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TOSYLPHENYLALANYL CHLOROMETHYL KETONE; TRYPSIN;

EID: 28844501811     PISSN: 16159306     EISSN: None     Source Type: Journal    
DOI: 10.1002/jssc.200500171     Document Type: Article

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