Non-fusion expression in Escherichia coli: Single-step purification of recombinant human annexin A5 for detection of apoptosis

Protein Expression and Purification

Volumn 45, Issue 1, 2006, Pages 80-87

  Wang, Fang ^a   He, Xiao Wen ^a   Yan, Hong Li ^a   Huang, Jing Jing ^a   Zhang, Yi ^a   Jiang, Lei ^a   Gao, Yuan Jian ^a   Sun, Shu Han ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Annexin A5; Apoptosis; DEAE Sepharose anion exchange chromatography; Magnetic beads

Indexed keywords

CALCIUM; DIAGNOSTIC AGENT; LIPOCORTIN 5; PHOSPHOLIPID; RECOMBINANT PROTEIN;

APOPTOSIS; ARTICLE; CELL CULTURE; CELL LINE; CHEMISTRY; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; HUMAN; IN VITRO STUDY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PHYSIOLOGY;

ANNEXIN A5; APOPTOSIS; CALCIUM; CELL LINE; CELLS, CULTURED; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; PHOSPHOLIPIDS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 28844491078     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.05.017     Document Type: Article

References (17)

1. C.M. Lahorte, J.L. Vanderheyden, N. Steinmetz, C. Van deWiele, R.A. Dierckx, and G. Slegers Apoptosis-detecting radioligands: current state of the art and future perspectives Eur. J. Nucl. Med. Mol. Imaging 31 2004 887 919
2. R. Gonzalez-Conejero, J. Corral, and V. Roldan A common polymorphism in the annexin V Kozak sequence(-1C > T) increases translation efficiency and plasma levels of annexin V, and decreases the risk of myocardial infarction in young patients Blood 100 2002 2081 2086
3. J.F. Tait, D. Gibson, and K. Fujikawa Phospholipid binding properties of human placental anticoagulant protein-I, a member of the lipocortin family J. Biol. Chem. 264 1989 7944 7949
4. A.G. Buckland, and D.C. Wilton Inhibition of human cytosolic phospholipase A2 by human annexin V Biochem. J. 329 1998 369 372
5. I. Vermes, C. Haanen, and H. Steffens-Nakken A novel assay for apoptosis flow cytometric detection of phosphatidylserine annexin V J. Immunol. Methods 184 1995 39 51
6. F. Blankenberg, C. Mari, and H.W. Strauss Imaging cell death in vivo Q. J. Nucl. Med. 47 2003 337 348
7. B.L. Kietselaer, L. Hofstra, E.A. Dumont, C.P. Reutelingsperger, and G.A. Heidendal The role of labeled Annexin A5 in imaging of programmed cell death. From animal to clinical imaging Q. J. Nucl. Med. 47 2003 349 361
8. T. Belhocine, N. Steinmetz, A. Green, and P. Rigo In vivo imaging of chemotherapy-induced apoptosis in human cancers Ann. N.Y. Acad. Sci. 1010 2003 525 529
9. J. Romisch, and N. Heimburger Purification and characterization of six annexins from human placenta Biol. Chem. 371 1990 383 388
10. T. Funakoshi, R.L. Heimark, L.E. Hendrickson, B.A. McMullen, and K. Fujikawa Human placental anticoagulant protein: isolation and characterisation. Biochemistry 26 1987 5572 5578
11. G.G. Poghosyan, V.Z. Melkonyan, M.V. Mikaelyan, and V.K. Gasparyan A simplified method for purification of annexin V from human placenta Prep. Biochem. Biotechnol. 33 2003 209 215
12. 1 Protein Expr. Purif. 34 2004 68 74
13. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
14. H. Towbin, T. Staehelin, and J. Gordon Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc. Natl. Acad. Sci. USA 76 1979 4350 4354
15. H.H. Boersma, L.M.L. Stolk, H. Kenis, N.M. Deckers, and J.L. Vanderheyden The ApoCorrect assay: a novel, rapid method to determine the biological functionality of radiolabeled and fluorescent annexin A5 Anal. Biochem. 327 2004 126 134
16. S.J. Martin, C.P. Reutelingsperger, A.J. McGahon, J.A. Rader, R.C. van Schie, D.M. LaFace, and D.R. Green Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Ab1 J. Exp. Med. 182 1995 1545 1556
17. J.F. Tait, D.F. Gibson, and C. Smith Measurement of the affinity and cooperativity of annexin V-membrane binding under conditions of low membrane occupancy Anal. Biochem. 329 2004 112 119