Purification of tubulin from limited volumes of cultured cells

Protein Expression and Purification

Volumn 45, Issue 1, 2006, Pages 183-190

  Fourest Lieuvin, Anne ^a  

^a CEA GRENOBLE   (France)

Author keywords

HeLa; Microtubule; Purification; Tubulin

Indexed keywords

TUBULIN;

ARTICLE; CELL CULTURE; CHEMISTRY; HELA CELL; HUMAN; ISOLATION AND PURIFICATION; MICROTUBULE; SENSITIVITY AND SPECIFICITY;

CELLS, CULTURED; HELA CELLS; HUMANS; MICROTUBULES; SENSITIVITY AND SPECIFICITY; TUBULIN;

EID: 28844487532     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.05.011     Document Type: Article

References (28)

1. R. Heald, and E. Nogales Microtubule dynamics J. Cell Sci. 115 2002 3 4
2. J.A. Hadfield, S. Ducki, N. Hirst, and A.T. McGown Tubulin and microtubules as targets for anticancer drugs Prog. Cell Cycle Res. 5 2003 309 325
3. R.C. Weisenberg, G.G. Borisy, and E.W. Taylor The colchicine-binding protein of mammalian brain and its relation to microtubules Biochemistry 7 1968 4466 4479
4. M.L. Shelanski, F. Gaskin, and C.R. Cantor Microtubule assembly in the absence of added nucleotides Proc. Natl. Acad. Sci. USA 70 1973 765 768
5. R.B. Vallee Reversible assembly purification of microtubules without assembly-promoting agents and further purification of tubulin, microtubule-associated proteins, and MAP fragments Methods Enzymol. 134 1986 89 104
6. R.C. Williams Jr., and J.C. Lee Preparation of tubulin from brain Methods Enzymol. 85 Pt. B 1982 376 385
7. D.L. Sackett Rapid purification of tubulin from tissue and tissue culture cells using solid-phase ion exchange Anal. Biochem. 228 1995 343 348
8. J.C. Bulinski, and G.G. Borisy Self-assembly of microtubules in extracts of cultured HeLa cells and the identification of HeLa microtubule-associated proteins Proc. Natl. Acad. Sci. USA 76 1979 293 297
9. J.A. Weatherbee, R.B. Luftig, and R.R. Weihing Purification and reconstitution of HeLa cell microtubules Biochemistry 19 1980 4116 4123
10. R.B. Vallee A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs) J. Cell Biol. 92 1982 435 442
11. R.B. Vallee, and C.A. Collins Purification of microtubules and microtubule-associated proteins from sea urchin eggs and cultured mammalian cells using taxol, and use of exogenous taxol-stabilized brain microtubules for purifying microtubule-associated proteins Methods Enzymol. 134 1986 116 127
12. C.N. Newton, J.G. DeLuca, R.H. Himes, H.P. Miller, M.A. Jordan, and L. Wilson Intrinsically slow dynamic instability of HeLa cell microtubules in vitro J. Biol. Chem. 277 2002 42456 42462
13. N. Caudron, O. Valiron, Y. Usson, P. Valiron, and D. Job A reassessment of the factors affecting microtubule assembly and disassembly in vitro J. Mol. Biol. 297 2000 211 220
14. M. Bornens, and M. Moudjou Studying the composition and function of centrosomes in vertebrates Methods Cell Biol. 61 1999 13 34
15. T. Mitchison, and M. Kirschner Microtubule assembly nucleated by isolated centrosomes Nature 312 1984 232 237
16. A. Lieuvin, J.C. Labbe, M. Doree, and D. Job Intrinsic microtubule stability in interphase cells J. Cell Biol. 124 1994 985 996
17. V. Gache, M. Louwagie, J. Garin, N. Caudron, L. Lafanechere, and O. Valiron Identification of proteins binding the native tubulin dimer Biochem. Biophys. Res. Commun. 327 2005 35 42
18. L. Paturle, J. Wehland, R.L. Margolis, and D. Job Complete separation of tyrosinated, detyrosinated, and nontyrosinatable brain tubulin subpopulations using affinity chromatography Biochemistry 28 1989 2698 2704
19. L. Guillaud, C. Bosc, A. Fourest-Lieuvin, E. Denarier, F. Pirollet, L. Lafanechere, and D. Job STOP proteins are responsible for the high degree of microtubule stabilization observed in neuronal cells J. Cell Biol. 142 1998 167 179
20. E. Denarier, A. Fourest-Lieuvin, C. Bosc, F. Pirollet, A. Chapel, R.L. Margolis, and D. Job Nonneuronal isoforms of STOP protein are responsible for microtubule cold stability in mammalian fibroblasts Proc. Natl. Acad. Sci. USA 95 1998 6055 6060
21. D. Job, M. Pabion, and R.L. Margolis Generation of microtubule stability subclasses by microtubule-associated proteins: implications for the microtubule "dynamic instability" model J. Cell Biol. 101 1985 1680 1689
22. T. Mitchison, and M. Kirschner Dynamic instability of microtubule growth Nature 312 1984 237 242
23. T. Horio, and H. Hotani Visualization of the dynamic instability of individual microtubules by dark-field microscopy Nature 321 1986 605 607
24. R.A. Walker, E.T. O'Brien, N.K. Pryer, M.F. Soboeiro, W.A. Voter, H.P. Erickson, and E.D. Salmon Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies J. Cell Biol. 107 1988 1437 1448
25. D. Panda, H.P. Miller, A. Banerjee, R.F. Luduena, and L. Wilson Microtubule dynamics in vitro are regulated by the tubulin isotype composition Proc. Natl. Acad. Sci. USA 91 1994 11358 11362
26. A. Banerjee, M.C. Roach, P. Trcka, and R.F. Luduena Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin J. Biol. Chem. 265 1990 1794 1799
27. A. Banerjee, M.C. Roach, P. Trcka, and R.F. Luduena Preparation of a monoclonal antibody specific for the class IV isotype of beta-tubulin. Purification and assembly of alpha beta II, alpha beta III, and alpha beta IV tubulin dimers from bovine brain J. Biol. Chem. 267 1992 5625 5630
28. Q. Lu, and R.F. Luduena In vitro analysis of microtubule assembly of isotypically pure tubulin dimers. Intrinsic differences in the assembly properties of alpha beta II, alpha beta III, and alpha beta IV tubulin dimers in the absence of microtubule-associated proteins J. Biol. Chem. 269 1994 2041 2047