A β-N-acetylhexosaminidase from Symbiobacterium thermophilum; Gene cloning, overexpression, purification and characterization

Enzyme and Microbial Technology

Volumn 38, Issue 3-4, 2006, Pages 457-464

  Ogawa, Masahiro ^a   Kitagawa, Mai ^a   Tanaka, Hideharu ^a   Ueda, Kenji ^a   Watsuji, Tomo O ^a   Beppu, Teruhiko ^a   Kondo, Akihiro ^a   Kawachi, Ryu ^a   Oku, Tadatake ^a   Nishio, Toshiyuki ^a  

^a NIHON UNIVERSITY   (Japan)

Author keywords

N acetylhexosaminidase; Enzyme characterization; Enzyme purification; Gene cloning; Symbiobacterium thermophilum

Indexed keywords

CLONING; DNA; ENZYME KINETICS; ESCHERICHIA COLI; GENES; THERMODYNAMIC STABILITY;

ENZYME CHARACTERIZATION; ENZYME PURIFICATION; GENE CLONING; SYMBIOBACTERIUM THERMOPHILUM;

ENZYMES;

4 NITROPHENYL BETA ACETYL BETA DEXTRO GALACTOSAMINIDE; 4 NITROPHENYL BETA GLUCOSIDE; 4 NITROPHENYL N ACETYL BETA DEXTRO GLUCOSAMINIDASE; AMINO ACID; BACTERIAL ENZYME; BETA N ACETYLHEXOSAMINIDASE; CHITIN; DNA FRAGMENT; GALACTOSAMINE; GENE PRODUCT; GLUCOSIDE; GLYCOSIDASE; OLIGOSACCHARIDE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ALKALINITY; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL CELL; CELL CULTURE; CELL FREE SYSTEM; CHROMOSOME; DNA SEQUENCE; DNA TEMPLATE; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE CONSTRUCT; GENE OVEREXPRESSION; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN FAMILY; SYMBIOBACTERIUM THERMOPHILUM; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SYMBIOBACTERIUM THERMOPHILUM;

EID: 28844449234     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2005.07.009     Document Type: Article

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