A homogeneous FRET assay system for multiubiquitin chain assembly and disassembly

Methods in Enzymology

Volumn 399, Issue , 2005, Pages 663-682

  Gururaja, Tarikere L ^a   Pray, Todd R ^b   Lowe, Raymond ^b   Dong, Guoqiang ^a   Huang, Jianing ^a   Daniel Issakani, Sarkiz ^a   Payan, Donald G ^a  

^a RIGEL PHARMACEUTICALS INC   (United States)

^b Acumen Pharmaceuticals Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCEIN; PEPTIDASE; POLYMER; PROTEASOME; UBIQUITIN;

BIOGENESIS; CATALYSIS; CONJUGATION; DEPOLYMERIZATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; EUKARYOTIC CELL; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROLYSIS; KINETICS; LIGATION; MONITORING; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN TARGETING; PURIFICATION; REVIEW;

BASE SEQUENCE; BIOPOLYMERS; BLOTTING, WESTERN; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENCE RESONANCE ENERGY TRANSFER; RECOMBINANT PROTEINS; UBIQUITIN;

EUKARYOTA;

EID: 28844449189     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(05)99044-7     Document Type: Review

References (40)

1. Baek K.H. Conjugation and deconjugation of ubiquitin regulating the destiny of proteins Exp. Mol. Med. 35 2003 1 7
2. Boisclair M.D., McClure C., Josiah S., Glass S., Bottomley S., Kamerkar S., and Hemmila I. Development of a ubiquitin transfer assay for high throughput screening by fluorescence resonance energy transfer J. Biomol. Screen. 5 2000 319 328
3. Cai S.Y., Babbitt R.W., and Marchesi V.T. A mutant deubiquitinating enzyme (Ubp-M) associates with mitotic chromosomes and blocks cell division Proc. Natl. Acad. Sci. USA 96 1999 2828 2833
4. Clegg R.M. Fluorescence resonance energy transfer (FRET) X.F. Wang B. Herman "Fluorescence Imaging Spectroscopy and Microscopy" 1996 John Wiley and Sons New York 179 252
5. Colbert D.L., and Childerstone M. Multiple drugs of abuse in urine detected with a single reagent and fluorescence polarization Clin. Chem. 33 1987 1921 1923
6. Dang L.C., Melandri F.D., and Stein R.L. Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes Biochemistry 37 1998 1868 1879
7. Davydov I.V., Woods D., Safiran Y.J., Oberoi P., Fearnhead H.O., Fang S., Jensen J.P., Weissman A.M., Kenten J.H., and Vousden K.H. Assay for ubiquitin ligase activity: High-throughput screen for inhibitors of HDM2 J. Biomol. Screen. 9 2004 695 703
8. Earnshaw D.L., Moore K.J., Greenwood C.J., Djaballah H., Jurewicz A.J., Murray K.J., and Pope A.J. Time-resolved fluorescence energy transfer DNA helicase assays for high throughput screening J. Biomol. Screen. 4 1999 239 248
9. Fairclough R.H., and Cantor C.R. The use of singlet-singlet energy transfer to study macromolecular assemblies Methods Enzymol. 48 1978 347 379
10. Fang S., and Weissmann A.M. Ubiquitin-proteasome system: A field guide to ubiquitylation Cell Mol. Life Sci. 61 2004 1546 1561
11. Finley D., Ciechanover A., and Varshavsky A. Ubiquitin as a central cellular regulator Cell 116 Suppl. 2 2004 S29 S32 2 p following S32.
12. Haugland R.P. "Handbook of Fluorescent Probes and Research Products." 9th Ed. 2002 Molecular Probes Inc. Eugene, OR
13. Hershko A., and Ciechanover A. The ubiquitin system Annu. Rev. Biochem. 67 1998 425 479
14. Hochstrasser M. Ubiquitin-dependent protein degradation Annu. Rev. Genet. 30 1996 405 439
15. Hong C.A., Swearingen E., Mallari R., Gao X., Cao Z., North A., Young S.W., and Huang S.G. Development of a high throughput time-resolved fluorescence resonance energy transfer assay for TRAF6 ubiquitin polymerization Assay Drug Dev. Technol. 1 2003 175 180
16. Huang J., Sheung J., Dong G., Coquilla C., Daniel-Issakani S., and Payan D.G. High-throughput screening for inhibitors of ubiquitin ligase APC Methods Enzymol. 399 2005 739 753
17. Kane S.A., Fleener C.A., Zhang Y.S., Davis L.J., Musselman A.L., and Huang P.S. Development of a binding assay for p53/HDM2 by using homogeneous time-resolved fluorescence Anal. Biochem. 278 2000 29 38
18. Kolb J.M., Yamanaka G., and Manly S.P. Use of a novel homogeneous fluorescent technology in high throughput screening J. Biomol. Screen. 1 1996 203 210
19. Lai Z., Yang T., Kim Y.B., Sielecki T.M., Diamond M.A., Strack P., Rolfe M., Caligiuri M., Benfield P.A., Auger K.R., and Copeland R.A. Differentiation of Hdm2-mediated p53 ubiquitination and Hdm2 autoubiquitination activity by small molecular weight inhibitors Proc. Natl. Acad. Sci. USA 99 2002 14734 14739
20. Lakowicz J.R. "Principles of Fluorescence Spectroscopy" 1999 Kluwer Academic/Plenum Publishers New York
21. Laney J.D., and Hochstrasser M. Assaying protein ubiquitination in Saccharomyces cerevisiae Methods Enzymol. 351 2002 248 257
22. Owicki J.C. Fluorescence polarization and anisotropy in high throughput screening: Perspectives and primer J. Biomol. Screen. 5 2000 297 306
23. Pickart C.M. Targeting substrates to the 26S proteasome FASEB J. 11 1997 1055 1066
24. Pickart C.M. Mechanisms underlying ubiquitination Annu. Rev. Biochem. 70 2001 505 533
25. Pray T.R., Parlati F., Huang J., Wong B.R., Payan D.G., Bennett M.K., Issakani S.D., Molineaux S., and Demo S.D. Cell cycle regulatory E3 ubiquitin ligases as anticancer targets Drug Resist. Update 5 2002 249 258
26. Robinson P.A. Ubiquitin-protein ligases-novel therapeutic targets? Curr. Protein Pept. Sci. 5 2004 163 176
27. Selvin P.R. Flourescence resonance energy transfer Methods Enzymol 246 1995 300 334
28. Selvin P.R., Rana T.M., and Hearst J.E. Luminescence resonance energy transfer J. Am. Chem. Soc. 116 1994 6029
29. Sun Y. Targeting E3 ubiquitin ligases for cancer therapy Cancer Biol. Ther. 2 2003 623 629
30. Sun L., and Chen Z.J. The novel functions of ubiquitination in signaling Curr. Opin. Cell Biol. 16 2004 119 126
31. Szollosi J., Damjanovich S., and Matyus L. Application of fluorescence resonance energy transfer in the clinical laboratory: Routine and research Cytometry 34 1998 159 179
32. Takada K., Nasu H., Hibi N., Tsukada Y., Ohkawa K., Fujimuro M., Sawada H., and Yokosawa H. Immunoassay for the quantification of intracellular multi-ubiquitin chains Eur. J. Biochem. 233 1995 42 47
33. Tang Z., Li B., Bharadwaj R., Zhu H., Ozkan E., Hakala K., Deisenhofer J., and Yu H. APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex Mol. Biol. Cell 12 2001 3839 3851
34. VanDemark A.P., and Hill C.P. Structural basis of ubiquitylation Curr. Opin. Struct. Biol. 12 2002 822 830
35. Wee K.E., Lai Z., Auger K.R., Ma J., Horiuchi K.Y., Dowling R.L., Dougherty C.S., Corman J.I., Wynn R., and Copeland R.A. Steady-state kinetic analysis of human ubiquitin-activating enzyme (E1) using a fluorescently labeled ubiquitin substrate J. Protein Chem. 19 2000 489 498
36. Wilkinson K.D. Regulation of ubiquitin-dependent processes by deubiquitinating enzymes FASEB J. 11 1997 1245 1256
37. Wilkinson K.D. Ubiquitination and deubiquitination: Targeting of proteins for degradation by the proteasome. Semin Cell Dev. Biol. 11 2000 141 148
38. Wong B.R., Parlati F., Qu K., Demo S., Pray T., Huang J., Payan D.G., and Bennett M.K. Drug discovery in the ubiquitin regulatory pathway Drug Discov. Today 8 2003 746 754
39. Wu P., and Brand L. Resonance energy transfer: Methods and applications Anal. Biochem. 218 1994 1 13
40. Yabuki N., Watanabe S., Kudoh T., Nihira S., and Miyamato C. Application of homogeneous time-resolved fluorescence (HTRFTM) to monitor poly-ubiquitination of wild-type p53 Comb. Chem. High Throughput Screen. 2 1999 279 287