Cytoplasmic expression of mature glycylglycine endopeptidase lysostaphin with an amino terminal hexa-histidine in a soluble and catalytically active form in Escherichia coli

Protein Expression and Purification

Volumn 45, Issue 1, 2006, Pages 206-215

  Sharma, Rahul ^b   Sharma, Poonam R ^b   Choudhary, Manohar L ^a   Pande, Amit ^a   Khatri, Ghan Shyam ^a,b  

^a Bharat Biotech Foundation   (India)

^b CSIR INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY   (India)

Author keywords

Expression; Glycylglycine endopeptidase; Lysostaphin; Metalloprotease; Purification; Staphylococcus simulans

Indexed keywords

HISTIDINE; HYBRID PROTEIN; LYSOSTAPHIN;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CHEMISTRY; CLASSIFICATION; COMPARATIVE STUDY; CYTOPLASM; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; PH; PHYLOGENY; SOLUBILITY; STAPHYLOCOCCUS; STRUCTURE ACTIVITY RELATION; TEMPERATURE; TIME;

AMINO ACID SEQUENCE; CATALYSIS; CLONING, MOLECULAR; CYTOPLASM; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HISTIDINE; HYDROGEN-ION CONCENTRATION; LYSOSTAPHIN; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PHYLOGENY; RECOMBINANT FUSION PROTEINS; SOLUBILITY; STAPHYLOCOCCUS; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE; TIME FACTORS;

ESCHERICHIA COLI; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS SIMULANS;

EID: 28844445069     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.025     Document Type: Article

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