Novel and simple method for isolating autologous mesothelial cells from the tunica vaginalis

BJU International

Volumn 96, Issue 9, 2005, Pages 1409-1413

  Asano, Touko ^a   Takazawa, Ryoji ^a   Yamato, Masayuki ^b   Kageyama, Yukio ^a   Kihara, Kazunori ^a   Okano, Teruo ^b  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^b TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

Author keywords

Mesothelial cells; Postoperative adhesions; Transplantation; Tunica vaginalis

Indexed keywords

CYTOKERATIN; VIMENTIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL ISOLATION; CELL SURFACE; CELL TRANSPLANTATION; CONTROLLED STUDY; HERNIA; MALE; MESOTHELIUM CELL; MICROVILLUS; NONHUMAN; PERITONEUM ADHESION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; TECHNIQUE; TUNICA VAGINALIS;

ADHESIONS; ANIMALS; CELL SEPARATION; CELLS, CULTURED; EPITHELIAL CELLS; HERNIA; HUMANS; MALE; RATS; RATS, INBRED LEW; SURGICAL FLAPS; TESTIS; TRANSPLANTATION, AUTOLOGOUS;

EID: 28544437670     PISSN: 14644096     EISSN: 1464410X     Source Type: Journal    
DOI: 10.1111/j.1464-410X.2005.05816.x     Document Type: Article

References (17)

1. Menzies D, Ellis H. Intestinal obstruction from adhesions - how big is the problem? Ann R Coll Surg Engl 1990; 72: 60-3
2. de Lange JJ. Prevalence of adhesions and the associated costs in general surgery. In diZerega GS ed, Peritoneal Surgery. New York: Springer Verlag, 2000: 307-20
3. Parker MC, Ellis H, Moran BJ et al. Postoperative adhesions: ten-year follow-up of 12,584 patients undergoing lower abdominal surgery. Dis Colon Rectum 2001; 44: 822-30
4. Stylianou E, Jenner LA, Davies M, Coles GA, Williams JD. Isolation, culture and characterization of human peritoneal mesothelial cells. Kidney Int 1990; 37: 1563-70
5. Mutsaers SE. Mesothelial cells: their structure, function and role in serosal repair. Respirology 2002; 7: 171-91
6. Horiuchi T, Miyamoto K, Miyamoto S et al. Image analysis of remesothelialization following chemical wounding of cultured human peritoneal mesothelial cells: the role of hyaluronan synthesis. Kidney Int 2003; 64: 2280-90
7. Di Paolo N, Sacchi G, Vanni L et al. Implant of autologous mesothelial cells in animals and a peritoneal dialysis patient. Int J Artif Organs 1989; 12: 485-501
8. Di Paolo N, Vanni L, Sacchi G. Autologous implant of peritoneal mesothelium in rabbits and man. Clin Nephrol 1990; 34: 179-84
9. Bertram P, Tietze L, Hoopmann M, Treutner KH, Mittermayer C, Schumpelick V. Intraperitoneal transplantation of isologous mesothelial cells for prevention of adhesions. Eur J Surg 1999; 165: 705-9
10. Wu YJ, Parker LM, Binder NE et al. The mesothelial keratins: a new family of cytoskeletal proteins identified in cultured mesothelial cells and nonkeratinizing epithelia. Cell 1982; 31: 693-703
11. Pronk A, Leguit P, Hoynck van Papendrecht AA, Hagelen E, van Vroonhoven TJ, Verbrugh HA. A cobblestone cell isolated from the human omentum: the mesothelial cell; isolation, identification, and growth characteristics. In Vitro Cell Dev Biol 1993; 29A: 127-34
12. Bird SD, Legge M, Walker RJ. Cultured peritoneal mesothelial cells exhibit apical primary cilia. Cell Biol Int 2004; 28: 79-92
13. Nicholson LJ, Clarke JM, Pittilo RM, Machin SJ, Woolf N. The mesothelial cell as a non-thrombogenic surface. Thromb Haemost 1984; 52: 102-4
14. Takazawa R, Yamato M, Kageyama Y, Okano T, Kihara K. Mesothelial cell sheets cultured on fibrin gel prevent adhesion formation in an intestinal hernia model. Tissue Eng 2005; 11: 618-25
15. Foley-Comer AJ, Herrick SE, Al-Mishlab T, Prele CM, Laurent GJ, Mutsaers SE. Evidence for incorporation of free-floating mesothelial cells as a mechanism of serosal healing. J Cell Sci 2002; 115: 1383-9
16. Park JM. Normal and anomalous development of the urogenital system. In Walsh PC, Retik AB, Vaughan ED Jr, Wein AJ eds, Campbell's Urology, 8th edn, Vol. III. Philadelphia: WB Saunders Inc., 2002: 1737-64
17. diZerega GS. Peritoneum, peritoneal healing and adhesion formation. In diZerega, GS ed., Peritoneal Surgery. New York: Springer Verlag, 2000: 3-37