HIV-1 western blot assay: What determines an indeterminate status?

Indian Journal of Medical Sciences

Volumn 59, Issue 10, 2005, Pages 443-450

  Syed, Iqbal ^a   Balakrishnan, P ^a,d   Solomon, Sunil ^a   Murugavel, K ^a   Kumarasamy, N ^a   Vidya, S ^a   Martin, S ^a   Thyagarajan, S ^b   Mayer, Kenneth ^c   Solomon, S ^a  

^a VHS Medical Centre India   (India)

^b UNIVERSITY OF MADRAS   (India)

^c BROWN UNIVERSITY   (United States)

^d YRG CENTRE FOR AIDS RESEARCH AND EDUCATION   (India)

Author keywords

HIV diagnosis; Indeterminate; Interpretation criteria; Western blot

Indexed keywords

HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY;

ARTICLE; CONTROLLED STUDY; DIAGNOSTIC VALUE; HIGH RISK POPULATION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NONHUMAN; RETROSPECTIVE STUDY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DIAGNOSIS; WESTERN BLOTTING;

EID: 28444436631     PISSN: 00195359     EISSN: None     Source Type: Journal    
DOI: 10.4103/0019-5359.17051     Document Type: Article

References (28)

1. Weber B, Hess G, Enzensberger R, Harms F, Evans CJ, Hamann A, et al. Multicenter evaluation of the novel ABN Western blot (immunoblot) system in comparison with an enzyme-linked immunosorbent assay and a different Western blot. J Clin Microbiol. 1992;30:691-7.
2. Lipniacki A, Piasek A.[Laboratory tests for HIV infection] Przegl Lek. 2003;60:478-84.
3. Grady C, Vogel S. Laboratory methods for diagnosing and monitoring HIV infection. J Assoc Nurses AIDS Care. 1993;4:11-21.
4. Chronister CL. Serologic confirmation of HIV infection. Optom Vis Sci. 1995;72:299-301.
5. Meles H, Wolday D, Fontanet A, Tsegaye A, Tilahun T, Aklilu M, et al. Indeterminate human immunodeficiency virus Western blot profiles in ethiopians with discordant screening-assay results. Clin Diagn Lab Immunol. 2002;9:160-3.
6. Celum CL, Coombs RW, Jones M, Murphy V, Fisher L, Grant C, et al. Risk factors for repeatedly reactive HIV-1 EIA and indeterminate western blots. A population-based case-control study. Arch Intern Med. 1994;154:1129-37.
7. Gastaldello R, Gallego S, Isa MB, Maturano E, Sileoni S, Nates S, et al. Immunoflurescence assay reactivity patterns of serum specimens presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina. Rev Inst Med Trop Sao Paulo. 2001;43:277-82.
8. Silverstein DM, Aviles DH, Vehaskari VM. False-positive human immunodeficiency virus antibody test in a dialysis patient. Pediatr Nephrol. 2004;19:547-9.
9. Center for Disease control. Interpretation and use of the Western blot assay for serodiagnosis of human immunodeficiency virus type 1 infections. Morbid. Mortal. Weekly Rep 1989;38:1-7.
10. Genesca J, Shih JW, Jett BW, Hewlett IK, Epstein JS, Alter HJ. What do western blot indeterminate patterns for human immunodeficiency virus mean in EIA-negative blood donors? Lancet 1989;2:1023-5.
11. Mac Donald KL, Jackson JB, Bowman RJ, Polesky HF, Rhame FS, Balfour HH et al. Performance characteristics of serologic tests for human immunodeficiency virus type 1 (HIV-1) antibody among Minnesota blood donors. Public health and clinical implications. Ann Intern Med 1989;110:617-21.
12. Schwartz. J.S.P.E.Dans, and B.P.Kinosian. Human Immunodeficiency virus test evaluation, performace, and use. JAMA 1998;259:2574-9.
13. Busch MP, Kleinman SH, Williams AE, Smith JW, Ownby HE, Laycock ME, et al. Frequency of human immunodeficiency virus (hiv) infection among contemporary anti-hiv-1 and anti-hiv-1/2 supplemental test-indeterminate blood donors. The retrovirus epidemiology donor study. Transfusion 1996;36:37-44.
14. Richard LD, Denise M, Holodniy M, Mole L, Margolis D, Blattner W, et al. Evaluation of Chiron HIV-1.HIV-2 Recombinant Immunoblot Assay. J Clin Microbiol 1996:34;2650-3.
15. Soriano V, Concheiro CL, Gutierrez M, Tuset C, Martinez-Zapico R, Ortiz de Lejarazu R, et al. Evaluation of various criteria for the interpretation of western blot for the diagnosis of human immunodeficiency virus infection. Spanish Group for the Study of HIV-2 Med Clin (Barc) 1993;100:561-6.
16. Mylonakis E, Paliou M, GreenBough TC, Flaningan TP, Letvin NL, Rich JD. Report of a False-positive HIV test result and the potential use of additional tests in establishing HIV Serostatus. Arch Intern Med 2000;160:2386-8.
17. Schochetman G, George JR. Serologic Testing for the Detection of Human Immunodeficiency Virus Infection. In: AIDS Testing. Methodology and management Issues. Springer-Verlog: Atlanta, USA; 1992;48-78.
18. Gerald SJ, George R. AIDS Testing, Methodology and Management issues. Springer-Verlag: New York USA; 1992;60:90-105.
19. Josephson SL, Swack NS, Ramirez MT, Hausler WJ Jr. Investigation of Atypical Western Blot Reactivity Involving Core Proteins of Human Immunodeficiency Virus Type 1 Journal of Clinical Microbiology 1989;27:932-7.
20. Courouce AM, Muller JY, Richard D. False positive Western Blot reactions to human immunodeficiency virus in blood donors. Lancet 1986;2:921-2
21. Drabick JJ, Horning VL, Lennox JL, Coyne PE, Oster CN, Knight RD, et al. A retrospective analysis of diseases associated with indeterminate HIV western blot patterns. Mil Med 1991;156:93-6.
22. Lee JH. Follow-up investigation of indeterminate western blot results for antibody to human immunodeficiency virus type 1. J Formos Med Assoc 1994;93:283-8.
23. Chattopadhya D, Aggarwal RK, Baveja UK, Doda V, Kumari S. Evaluation of epidemiological and serological predictors of human immunodeficiency virus type-1 (HIV-1) infection among high risk professional blood donors with western blot indeterminate results. J Clin Virol 1998;11:39-49.
24. Kleinman S. Fitzpatrick L, Secord K, Wilke D. Follow-up testing and notificaton of anti-HIV Western blot atypical (indeterminate) donors. Transfusion 1998;28:280-2
25. Phair J, Hoover D, Huprikar J, Detels R, Kaslow R, Rinaldo C, et al. The significance of western blot assays indeterminate for antibody to HIV in a cohort of homosexual/bisexual men. The Multicenter AIDS Cohort Study. J Acquir Immune Defic Syndr 1992;5:988-92.
26. Dock NL, Lamberson HV, O' Brien TA, Tribe DE, Alexander SS, Poiesz BJ. Evaluation of atypical human immunodeficiency virus immunoblot reactivity in blood donors. Transfusion 1988;28:412-8.
27. Jackson JB, MacDonald KL, Cadwell J, Sullivan C, Kline WE, Hanson M, et al. Absence of HIV infection in blood donors with indeterminate western blot tests for antibody to HIV-1. N Engl J Med 1990;322:217-22.
28. Midthun K, Garrison L, Clements ML, Farzadegan H, Fernie B, Quinn T. Frequency of indeterminate western blot tests in healthy adults at low risk for human immunodeficiency virus infection. The NIAID AIDS Vaccine Clinical Trails Network. J Infect Dis 1990;162:1379-82.