A technique for the rapid extraction of microalgal DNA from single live and preserved cells

Molecular Ecology Notes

Volumn 5, Issue 3, 2005, Pages 688-691

  Richlen, M L ^a   Barber, P H ^a  

^a BOSTON UNIVERSITY MARINE PROGRAM   (United States)

Author keywords

Dinoflagellates; DNA extraction; Gambierdiscus; Harmful algal blooms; Single cell sequencing

Indexed keywords

ALGAE; DINOPHYCEAE; GAMBIERDISCUS; GAMBIERDISCUS TOXICUS;

EID: 28344441261     PISSN: 14718278     EISSN: 14718286     Source Type: Journal    
DOI: 10.1111/j.1471-8286.2005.01032.x     Document Type: Article

References (25)

1. Adachi R, Fukuyo Y (1979) The thecal structure of a marine toxic dinoflagellate Gambierdiscus toxicus gen et sp. nov. collected in a ciguatera endemic area. Bulletin of the Japanese Society of Science Fish, 45, 67-71.
2. Bucklin A, Allen LD (2004) mtDNA sequencing from zooplankton after long-term preservation in buffered formalin. Molecular Phylogenetics and Evolution, 30, 879-882.
3. Chinain M, Germain M, Sako Y, Pauillac S, Legrand A (1998) Genetic diversity in French Polynesian strains of the ciguatera-causing dinoflagellate Gambierdiscus toxicus: RFLP and sequence analyses on the SSU and LSU rRNA genes. Harmful Algae.
4. Chinain M, Revel T, Holmes MJ, Ung A, Pauillac S (2001) Molecular characterization and classification of the ciguatera dinoflagellate Gambierdiscus. In: Harmful Algal Blooms 2000 (eds Hallegraeff GM, Blackburn SI, Bolch CJ, Lewis RJ), Intergovernmental Oceanographic Commission of UNESCO, Paris, France.
5. Edvardsen B, Shalchian-Tabrizi K, Jakobsen KS et al. (2003) Genetic variability and molecular phylogeny of Dinophysis (Dinophyceae) species from Norwegian waters inferred from single cell analyses of rDNA. Journal of Phycology, 39, 395-408.
6. Godhe A, Anderson DM, Rehnstam-Holm A-S (2002a) PCR amplification of microalgal DNA for sequencing and species identification: studies on fixatives and algal growth stages. Harmful Algae, 1, 375-382.
7. Godhe A, Rehnstam-Holm A-S, Karunasagar I, Karunasagar I (2002b) PCR detection of dinoflagellate cysts in field sediment samples from tropic and temperate environments. Harmful Algae, 1, 361-373.
8. Hallegraeff GM (2003a) Harmful algal blooms: a global overview. In: Manual on Harmful Marine Microalgae (eds Hallegraeff GM, Anderson DM, Cembella AD), pp. 25-49. UNESCO, Paris, France.
9. Hallegraeff GM (2003b) Taxonomic principles. In: Manual on Harmful Marine Microalgae (eds Hallegraeff GM, Anderson DM, Cembella AD). UNESCO, Landais, France.
10. John U, Fensome RA, Medlin LK (2003) The application of a molecular clock based on molecular sequences and the fossil record to explain biogeographic distributions within the Alexandrium tamarense 'species complex' (Dinophyceae). Molecular Biology and Evolution, 20, 1015-1027.
11. Legrand B, de Mazancourt P, Durigon M, Khalifat V, Crainic K (2002) DNA genotyping of unbuffered formalin fixed paraffin embedded tissues. Forensic Science International, 125, 205-211.
12. Lenaers G, Maroteaux L, Michot B, Herzog M (1989) Dinoflagellates in evolution. A molecular phylogenetic analysis of large subunit ribosomal RNA. Journal of Molecular Evolution, 29, 40-51.
13. Marín I, Aguilera A, Reguera B, Abad JP (2001) Preparation of DNA suitable for PCR amplification from fresh or fixed single dinoflagellate cells. BioTechniques, 30, 88-93.
14. Oldach DW, Delwiche CF, Jakobsen KS et al (2000) Heteroduplex mobility assay-guided sequence discovery: elucidation of the small subunit (18S) rDNA sequences of Pfiesteria piscicida and related dinoflagellates from complex algal culture and environmental sample DNA pools. PNAS, 97, 4303-4308.
15. Ruiz Sebastián C, O'Ryan C (2001) Single-cell sequencing of dinoflagellate (Dinophyceae) nuclear ribosomal genes. Molecular Ecology Notes, 1, 329-331.
16. Scholin CA, Anderson DM (1998) Detection and quantification of HAB species using antibody and DNA probes: progress to date and future research objectives. In: Harmful Algae Proceedings of the 8th International Conference on Harmful Algae (eds Reguera JB B, Fernández ML, Wyatt T), pp. 253-257. UNESCO, Paris.
17. Scholin CA, Herzog M, Sogin M, Anderson DM (1994b) Identification of group and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragment of the LSU rRNA gene. Journal of Phycology, 30, 999-1011.
18. Scholin C, Villac M, Buck K et al (1994a) Ribosomal DNA sequences discriminate among toxic and non-toxic Pseudonitzschia species. Natural Toxins, 2, 152-165.
19. Scholin CA, Vrieling E, Peperzak L, Rhodes L, Rublee P (2003) Detection of HAB species using lectin, antibody and DNA probes. In: Manual on Harmful Marine Microalgae (eds Hallegraeff GM, Anderson DM, Cembella AD), pp. 131-163. UNESCO, Paris, France.
20. Singer-Sam J, Tanguay RL, Riggs AD (1989) Use of Chelex to improve the PCR signal from a small number of cells. Amplifications: A Forum for PCR Users, 11.
21. Soller R, Warnke K, Saint-Paul U, Blohm D (2000) Sequence divergence of mitochondrial DNA indicates cryptic biodiversity in Octopus vulgaris and supports the taxonomic distinctiveness of Octopus mimus (Cephalopoda: Octopodidae). Marine Biology, 136, 29-35.
22. Uribe PC, Suarez-Isla BA, Espejo RT (1999) Ribosomal RNA heterogeneity and identification of toxic dinoflagellate cultures by heteroduplex mobility assay. Journal of Phycology, 35, 884-888.
23. Vernoux JP, Lejeune J (1994) Ciguatera in the French West Indies. Memoirs of the Queensland Museum, 34, 631-638.
24. Walsh SP, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques, 10, 506-513.
25. Wang Q, Deeds JR, Place AR, Belas R (2005) Dinoflagellate community analysis of a fish kill using denaturing gradient gel electrophoresis. Harmful Algae, 4, 151-162.