Rational engineering of a fluorescein-binding anticalin for improved ligand affinity

Biological Chemistry

Volumn 386, Issue 11, 2005, Pages 1097-1104

  Vopel, Sven ^a   Mühlbach, Hermine ^a   Skerra, Arne ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Circular dichroism; Fluorescence titration; Hapten; Ligand binding; Lipocalin; Protein design; Spectroscopy

Indexed keywords

ALANINE; ARGININE; BINDING PROTEIN; FLUORESCEIN; LIPOCALIN; PROTEIN; TYROSINE;

ARTICLE; BINDING AFFINITY; CIRCULAR DICHROISM; CRYSTAL STRUCTURE; DEFENSE MECHANISM; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN FOLDING;

BACTERIA; CALORIMETRY, DIFFERENTIAL SCANNING; CARRIER PROTEINS; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; FLUORESCEIN; LIGANDS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS;

INSECTA;

EID: 28244462439     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC.2005.126     Document Type: Article

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