Direct observation of biaxial confinement of a semiflexible filament in a channel

Macromolecules

Volumn 38, Issue 23, 2005, Pages 9882-9884

  Choi, M C ^a,b   Santangelo, C D ^c   Pelletier, O ^b   Kim, J H ^a   Kwon, S Y ^a,d   Wen, Z ^e   Li, Y ^b   Pincus, P A ^a,b   Safinya, C R ^b   Kim, M W ^a,b  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

^d CHONGQING UNIVERSITY   (China)

^e Korea Research Institute of Standards and Science   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; CORRELATION FUNCTION; VIDEO MICROSCOPES;

MICROSCOPIC EXAMINATION; OPTICAL RESOLVING POWER; TEMPERATURE DISTRIBUTION;

MACROMOLECULES;

EID: 28144460094     PISSN: 00249297     EISSN: None     Source Type: Journal    
DOI: 10.1021/ma051348n     Document Type: Article

References (14)

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10. 2, 0.5 mM ATP, and 0.2 mM DTT, was purchased from Cytoskeleton Inc., Denver, CO.
11. Pfohl, T.; Kim, J. H.; Yasa, M.; Miller, H. D.; Wong, G. C.; Bringezu, F.; Wen, Z.; Wilson, L.; Kim, M. W.; Li, Y.; Safinya, C. R. Langmuir 2001, 17, 5343.
12. Choi, M. C.; Pfohl, T.; Wen, Z.; Li, Y.; Kim, M. W.; Israelachivili, J. N.; Safinya, C. R. Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 17340.
13. SIT camera purchased from Dage-MTI Inc., Michigan, IN.
14. We assume that the angle between the tangent vector of F-actin and the sidewall is small. To verify this, the angular distributions were measured. The distributions fit well with a Gaussian curve centered at 0°, -6.5°, with its fwhm at 16°, 19°, respectively, for W = 3 and 5 μm. It can be interpreted that F-actin does not bend more than 8° (20°) from the sidewall of the 3 μm (5 μm) channel. However, in an unconfined regime (i.e., W ≥ 10 μm), F-actin has a broad angular distribution between -70° and 50°.