Characterization of the penicillin G acylase from Bacillus megaterium ATCC 14945

Brazilian Archives of Biology and Technology

Volumn 48, Issue SPEC. ISS., 2005, Pages 105-111

  De Souza, Vanessa Ribeiro ^a   Silva, Ana C G ^a   Pinotti, Laura Marina ^a   Selistre Araújo, Heloísa Sobreiro ^a   Camargo Giordano, Raquel De Lima ^a  

^a FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

Author keywords

Bacillus megaterium; Characterization; Penicillin G acylase

Indexed keywords

BACILLUS MEGATERIUM;

EID: 28044460622     PISSN: 15168913     EISSN: 15168913     Source Type: Journal    
DOI: 10.1590/s1516-89132005000400013     Document Type: Article

References (16)

1. th ed. New York : John Wiley.
2. Balasingham, K.; Warburton, D.; Dunnill, P. and Lilly, D. (1984), Enzymatic conversion in aqueous two-phase system: Deacylation of benzylpenicillin to 6-aminopenicilanic acid with penicillin acylase. Enzyme Microb. Technol., 6, 301-306.
3. Bradford, M. M. (1976), A rapid and sensitive method for the quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-54.
4. Cheang, B. and Zydney, A. (2003), Separation of α-Lacto albumin and β-Lacto globulin using membrane ultra filtration, Biotechnology and Bioengineering, 83 : (2), 201-209.
5. Duggleby, H. J.; Tolley, S. P.; Dodson, E. J. and Moody, P. C. E. (1995), Penicillin acylase has a single- amino-acid cata. Nature, 373, 264-8.
6. Gómez, A. et al. (1994), Strategies in the design of a Penicillin Acylase Process. Cuernavaca: Instituto de Biotecnologia.
7. Lehninger, A. L. (1991), Princípios de Bioquímca. São Paulo : Sarvier. pp. 127-194.
8. Martín, L.; Prieto, M. A.; Cortés, E. and García, J. L. (1995), Cloning and Sequencing of the pac Gene Encoding the Penicillin G Acylase of Bacillus megaterium ATCC 14945. FEMS Microbiology Letters, 125, 287-292.
9. Modesto, M. (2002), Estimativa de Parâmetros Cinéticos Inerentes de Enzimas Imobilizadas. Trabalho de graduação no curso de Engenharia Quimica da UFSCar, São Carlos.
10. Pinotti, L. M.; Silva, R. G.; Giordano, R. C. and Giordano, R. L. C. (2002), Inoculum Studies in Production of Penicillin G Acylase by Bacillus megaterium ATCC 14945. Applied Biochemistry and Biotechnology, Totowa, New Jersey, 98-100, 679-686.
11. Pinotti, L. M.; Silva, A. F. S.; Silva, R. G. and Giordano, R. L. C. (2000), Study of Different Media for Production of Penicillin G Acylase from Bacillus megaterium ATCC 14945. Applied Biochemistry and Biotechnology, Totowa, New Jersey - USA, 84-86, 655-677.
12. Savidge, T. A. and Cole, M. (1975), Penicillin Acylase (bacterial). Methods in Enzymology, 43, 705-721.
13. Segel, I. H. (1975), Enzyme Kinetics. New York, John Wiley and Sons, Inc., capítulos 1, 2, 3, 4 e 11.
14. Shewale, J. G. and Sivaraman, H. (1989), Penicillin Acylase: Enzyme production and its application in the manufacture of 6-APA, Proc. Biochem., 146-154.
15. Voet, J. G. (1995), Biochemistry. 2.ed. NY, USA : John Wiley and sons.
16. Konstantinovic, M.; Marjanovic, N.; Ljubijankic, G. and Glisin, V. (1994), The Penicillin amidase of Arthrobacter viscosus (ATCC 15294). Gene, 143, 79-83. (http://www.frost.com, mar. 2004).