Quantitative assessment of human β-globin gene expression in vitro by TaqMan real-time reverse transcription-PCR: Comparison with competitive reverse transcription-PCR and application to mutations or deletions in noncoding regions

Clinical Chemistry

Volumn 51, Issue 12, 2005, Pages 2395-2396

  Irenge, Leonid M ^a   Robert, Annie ^a   Gala, Jean Luc ^a,b  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

^b Defense Laboratories Department   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

6 CARBOXYFLUORESCEIN; BETA GLOBIN; CYCLOPHILIN A; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MESSENGER RNA; TAQ POLYMERASE;

5' UNTRANSLATED REGION; ARTICLE; BETA THALASSEMIA; COMPARATIVE STUDY; COMPETITIVE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; CONTROLLED STUDY; GENE DELETION; GENE EXPRESSION; GENE MUTATION; GENETIC TRANSFECTION; GLOBIN GENE; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REGULATOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBONUCLEASE PROTECTION ASSAY; STUDENT T TEST; WILD TYPE;

GLOBINS; HUMANS; MUTATION; OPEN READING FRAMES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE DELETION; TIME FACTORS;

EID: 28044444751     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2005.056630     Document Type: Article

References (9)

1. Weatherall DJ. The thalassaemias. BMJ 1997;314:1675-8.
2. Huisman TH, Carver M, Abyssal E. A syllabus of thalassemia mutations. Augusta, GA: The Sickle Cell Anemia Foundation, 1997:29pp.
3. Ho PJ, Sloane-Stanley J, Athanassiadou A, Wood WG, Thein SL. An in vitro system for expression analysis of mutations of the β-globin gene: validation and application to two mutations in the 5′ UTR. Br J Haematol 1999;106: 938-47.
4. Romao L, Inacio A, Santos S, Avila M, Faustino P, Pacheco P, et al. J. Nonsense mutations in the human β-globin gene lead to unexpected levels of cytoplasmic mRNA accumulation. Blood 2000;96:2895-901.
5. Sgourou A, Papachatzopoulou A, Psiouri L, Antoniou M, Zoumbos N, Gibbs R, et al. The β-globin C→G mutation at 6 bp 3′ to the termination codon causes β-thalassaemia by decreasing the mRNA level. Br J Haematol 2002;118: 671-6.
6. Irenge LM, Heusterspreute M, Philippe M, Derclaye I, Robert A, Gala JL. Validation of a recombinant DNA construct (micro LCR and full-length β-globin gene) for quantification of human β-globin expression: application to mutations in the promoter, intronic, and 5′- and 3′-untranslated regions of the human β-globin gene. Clin Chem 2002;48:1787-91.
7. Sgourou A, Routledge S, Antoniou M, Papachatzopoulou A, Psiouri L, Athanassiadou A. Thalassaemia mutations within the 5′UTR of the human β-globin gene disrupt transcription. Br J Haematol 2004;124:828-35.
8. Athanassiadou A, Papachatzopoulou A, Zoumbos N, Maniatis GM, Gibbs R. A novel β-thalassaemia mutation in the 5′ untranslated region of the β-globin gene. Br J Haematol 1994;88:307-10.
9. Dekairelle AF, Tombal B, Cosyns JP, Gala JL. Assessment of the transcriptional activity of p53 improves the prediction of recurrence in superficial transitional cell carcinoma (TCC) of the bladder. Clin Can Res 2005;11: 4724-32.