Purification and characterization of an α-galactosidase from Aspergillus fumigatus

Brazilian Archives of Biology and Technology

Volumn 48, Issue 2, 2005, Pages 195-202

  De Rezende, Sebastião Tavares ^a   Guimarães, Valéria Monteze ^a   De Rodrigues, Marília Castro ^b   Felix, Carlos Roberto ^b  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

^b UNIVERSITY OF BRASILIA   (Brazil)

Author keywords

Aspergillus fumigatus; Raffinose oligosaccharides; galactosidase

Indexed keywords

ASPERGILLUS FUMIGATUS;

EID: 27944510722     PISSN: 15168913     EISSN: 15168913     Source Type: Journal    
DOI: 10.1590/s1516-89132005000200005     Document Type: Article

References (22)

1. Ademark, P.; Larsson, M.; Tjerneld, F. and Stalbrand, H. (2001), Multiple α-galactosidases from Aspergillus niger: purification, characterization and substrate specificities. Enzyme Microb. Technol., 29, 441-448.
2. Adya, S. and Elbein, A. D. (1977), Glycoprotein enzymes secreted by Aspergillus niger: purification and properties of α-galactosidase. J. Bacteriol., 129, 850-856.
3. Bergmeyer, H. U. and Bernt, E. (1974), Determination of glucose with oxidase and peroxidase. In: Bergmeyer, H. U. (Eds.). Methods of Enzymatic Analysis. pp.1205-1215.
4. Blum, H., Beier, H. and Gross, H. (1987), Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis, 8, 93-99.
5. Bradford, M. M. (1976), A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle dye biding. Anal. Biochem., 72, 680-685.
6. Bulpin, P. V.; Gidley, M. J. and Jeffcoat, R. (1990), Development of a biotechnological process for the modification of galactomannan polymers with plant α-galactosidase. Carbohydr. Polym., 12, 155-168.
7. Dey, P. M. and Pridham, J. B. (1972), Biochemistry of α-galactosidases. Adv. Enzymol., 36, 91-130.
8. Duffaud, G. D.; McCutchen, M. C.; Leduc, P.; Parker, K. N. and Kelly, R. M. (1997), Purification and characterization of extremely thermostable β-mannosidase, and α-galactosidase from the hyperthermophilic Eubacterium Thermotoga neapolitana 5068. Appl. Environ. Microbiol., 63, 169-177.
9. Guimarães, V. M.; Rezende, S. T.; Moreira, M. A.; Barros, E. B. and Felix, C. R. (2001), Characterization of α-galactosidases from germinating soybean seed and their use for hydrolysis of oligosaccharides. Phytochemistry, 58, 67-63.
10. Kaneko, R.; Kusakabe, I.; Ida, E. and Murakami, K. (1991), Substrate specificity of α-galactosidase from Aspergillus niger 5-16. Agric. Biol. Chem., 55, 109-115.
11. Laemmli, U. K. (1970), Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature., 227, 680-683
12. Luonteri, E.;Alatalo, E.; Siika-aho, M.; Penttila, M. and Tenkanen, M. (1998), α-Galactosidases of Penicillium simplicimum: production, purification and characterization of the gene encoding AGLI. Biotechnol. Appl Biochem., 28, 179-188.
13. Miller, G. L. (1956), Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426-428.
14. Moser, M.; Menz, G.; Blaser, K. and Crameri, R. (1994), Recombinant expression and antigenic properties of a 32-kilodalton extracellular alkaline protease, representing possible virulence factor form Aspergillus fumigatus. Infect. Immun., 62, 936-942.
15. Rezende, S. T. and Felix C. R. (1997), Raffinose-Hydrolyzing activity of Aspergillus fumigatus. Biotechnol. Lett., 19, 217-220.
16. Rezende, S. T. and Felix C. R. (1999), Production and characterization of raffinose-hydrolynsing and invertase activities of Aspergillus fumigatus. Folia Microbiol., 44 : (2), 191-195.
17. Santos, R. M. B.; Firmino, A. A. P. and Felix, C. R. (1996), Keratinolytic activity of Aspergillus fumigatus Fresenius. Cur. Microbiol., 33, 364-370.
18. Shibuya, H.; Kobayashi, H.; Park, G. G.; Komatsu, Y.; Sato, T.; Kaneko, R.; Nagasaki, H.; Yoshida, S.; Kasamo, K. and Kusakabe, I. (1995), Purification and some properties of α-galactosidases from Penicillium purpurogenum. Biosci. Biotech. Biochem., 59, 2333-2335.
19. Varbanetes, L. D.; Malanchuk, V. M.; Buglova, T. T. and Kuhlmann, R. A. (2001), Penicillium sp. 23 α-galactosidase: purification and substrate specificity. Carbohydr. Polym., 44,357-363.
20. Xiao, M.; Tanaka, K.; Qian, X. M.; Yamamoto, K. and Kumagai, H. (2000), High-yield production and characterization of α-galactosidase from Bifidobacterium breve grown on raffinose. Biotechnol. Lett., 22 : (9), 747-751.
21. Zeilinger, S.; Kristufek, D.; Arissan-Atac, I.; Hodits, R. and Kubicek, C. P. (1993), Condition of formation, purification, and characterization of an α-galactosidase of Trichodrema reesei RUT C-30. Appl. Environ. Microbiol., 59, 1347-1353.
22. Zhu, A.; Leng, L.; Monahan, C.; Zhang, Z.; Hurst, R.; Lenny, L. and Goldstein, J. (1996), Characterization of recombinant α-galactosidase for use in seroconversion from blood group B to O of human erythrocytes. Arch. Biochem. Biophys., 327, 324-329.