Identification and molecular characterization of an N-acetylmuramyl-L- alanine amidase Sle1 involved in cell separation of Staphylococcus aureus

Molecular Microbiology

Volumn 58, Issue 4, 2005, Pages 1087-1101

  Kajimura, Junko ^a   Fujiwara, Tamaki ^a   Yamada, Sakuo ^b   Suzawa, Yoshika ^a   Nishida, Tetsuya ^a   Oyamada, Yoshihiro ^c   Hayashi, Ikue ^d   Yamagishi, Jun Ichi ^c   Komatsuzawa, Hitoshi ^a   Sugai, Motoyuki ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b KAWASAKI MEDICAL SCHOOL   (Japan)

^c Microbiology Group   (Japan)

^d HIROSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOLYSIN; BACTERIAL ENZYME; GENE PRODUCT; MUTANT PROTEIN; N ACETYLMURAMOYLALANINE AMIDASE; PEPTIDOGLYCAN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; AUTOLYSIS; BACTERIAL CELL; BACTERIOLYSIS; CELL SEPARATION; CHEMICAL BOND; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME PURIFICATION; GENE CLUSTER; GENE IDENTIFICATION; GENE INSERTION; GENE MUTATION; GENETIC LINKAGE; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; STAPHYLOCOCCUS AUREUS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; CELL DIVISION; DISEASE MODELS, ANIMAL; GENE DELETION; GENES, BACTERIAL; MICE; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, INSERTIONAL; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; PEPTIDOGLYCAN; PHENOTYPE; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; SUBSTRATE SPECIFICITY; VIRULENCE FACTORS;

ANIMALIA; BACTERIA (MICROORGANISMS); STAPHYLOCOCCUS AUREUS;

EID: 27944473534     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2005.04881.x     Document Type: Article

References (33)

1. Bateman, A., and Bycroft, M. (2000) The structure of a LysM domain from E. coli membrane-bound lytic murein transglycosylase D (MltD). J Mol Biol 299: 1113-1119.
2. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
3. De Las Rivas, B., Garcia, J.L., Lopez, R., and Garcia, P. (2002) Purification and polar localization of pneumococcal LytB, a putative endo-beta-N-acetylglucosaminidase: the chain-dispersing murein hydrolase. J Bacteriol 184: 4988-5000.
4. Ghuysen, J.M., Tipper, D.J., and Strominger, J.L. (1966) Enzymes that degrade bacterial cell walls. Methods Enzymol 8: 685-699.
5. Heidrich, C., Templin, M.F., Ursinus, A., Merdanovic, M., Berger, J., Schwarz, H., et al. (2001) Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli. Mol Microbiol 41: 167-178.
6. von Heijne, G. (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14: 4683-4690.
7. Heilmann, C., Thumm, G., Chhatwal, S., Hartleib, J., Uekotter, A., and Peters, G. (2003) Identification and characterization of a novel autolysin (Aae) with adhesive properties from Staphylococcus epidermidis. Microbiology 149: 2769-2778.
8. Höltje, J.V. (1998) Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli. Microbiol Mol Bio Rev 62: 181-203.
9. Komatsuzawa, H., Suzuki, J., Sugai, M., Miyake, Y., and Suginaka, H. (1994) The effect of Triton X-100 on the in vitro susceptibility of methicillin-resistant Staphylococcus aureus to oxacillin. J Antimicrob Chemother 34: 885-897.
10. Komatsuzawa, H., Sugai, M., Nakashima, S., Yamada, S., Matsumoto, A., Oshida, T., and Suginaka, H. (1997) Subcellular localization of the major autolysin, ATL and its processed proteins in Staphylococcus aureus. Microbiol Immunol 41: 469-479.
11. Mani, N., Baddour, L.M., Offutt, D.Q., Vijaranakul, U., Nadakavukaren, M.J., and Jayaswal, R.K. (1994) Autolysis-defective mutant of Staphylococcus aureus: pathological considerations, genetic mapping, and electron microscopic studies. Infect Immun 62: 1406-1409.
12. Nugroho, F.A., Yamamoto, H., Kobayashi, Y., and Sekiguchi, J. (1999) Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis. J Bacteriol 181: 6230-6237.
13. Ohnishi, R., Ishikawa, S., and Sekiguchi, J. (1999) Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis. J Bacteriol 181: 3178-3184.
14. Ohta, K., Komatsuawa, H., Sugai, M., and Suginaka, H. (1998) Zymographic characterization of Staphylococcus aureus cell wall. Microbiol Immunol 42: 231-235.
15. Oshida, T., Sugai, M., Komatsuzawa, H., Hong, Y.-M., Suginaka, H., and Tomasz, A. (1995) A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-β-N- acetylglucosaminidase domain: cloning, seuence analysis, and characterization. Proc Natl Acad Sci USA 92: 285-289.
16. Ramadurai, L., and Jayaswal, R.K. (1997) Molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. J Bacteriol 179: 3625-3631.
17. Roos, M., Pittenauer, E., Schmid, E., Beyer, M., Reinike, B., Allmaier, G., and Labischinski, H. (1998) Improved high-performance liquid chromatographic separation of peptidoglycan isolated from various Staphylococcus aureus strains for mass spectrometric characterization. J Chromatogr B Biomed Sci Appl 705: 183-192.
18. Sambrook, J., Maniatis, T., and Fritsch, E.F. (1989) Molecular Cloning: A Laboratory Manual. Plainview, New York: Cold Spring Harbor Laboratory Press.
19. Severin, A., Horne, D., and Tomaz, A. (1997) Autolysis and cell wall degradation in a choline-independent strain of Streptococcus pneumoniae. Microb Drug Resist 3: 391-400.
20. Shockman, J.D., and Barret, J.F. (1983) Structure, function, and assembly of cell walls of Gram-positive bacteria. Annu Rev Microbiol 37: 501-527.
21. Shockman, J.D., and Höltje, J.V. (1994) Microbial peptidoglycan (murein) hydrolases. In Bacterial Cell Wall, Vol. 27. Ghuysen, J.M., and Hakenbeck, R. (eds). Amsterdam: Elsevier Science B. V., pp. 131-166.
22. Stranden, A.M., Ehlert, K., Labischinski, H., and Berger-Baechi, B. (1997) Cell wall monoglycine cross-bridges and methicillin hypersusceptibility in a femAB null mutant of methicillin-resistant Staphylococcus aureus. J Bacteriol 179: 9-16.
23. Sugai, M. (1997) Peptidoglycan hydrolases of the Staphylococci. J lnfect Chemother 3: 113-127.
24. Sugai, M., Akiyama, T., Komatsuzawa, H., Miyake, Y., and Suginaka, H. (1990) Characterization of sodium dodecyl sulfate-stable Staphylococcus aureus bacteriolytic enzymes by polyacrylamide gel electrophoresis. J Bacteriol 172: 6494-6498.
25. Sugai, M., Komatsuawa, H., Tomita, S., Akiyama, T., Miyake, Y., and Suginaka, H. (1991) Detection of a staphylococcal endo-beta-N- acetylglucosaminidase using polyacrylamide gels. J Microbiol Methods 13: 11-16.
26. Sugai, M., Komatsuzawa, H., Akiyama, T., Hong, Y.-M., Oshida, T., Miyake, Y., et al. (1995) Identification of endo-β-N-acetylglucosaminidase and N-acetylmuramyl-L-alanine amidase as cluster-dispersing enzymes in Staphylococcus aureus. J Bacteriol 177: 1491-1496.
27. Sugai, M., Yamada, S., Nakashima, S., Komatsuawa, H., Matsumoto, A., Oshida, T., and Suginaka, H. (1997a) Localized perforation of the cell wall by a major autolysin: atl gene products and the onset of penicillin-induced lysis of Staphylococcus aureus. J Bacteriol 179: 2958-2962.
28. Sugai, M., Fujiwara, T., Ohta, K., Komatsuzawa, H., Ohara, M., and Suginaka, H. (1997b) epr, which encodes glycylglycine endopeptidase resistance, is homologous to femAB and affects serine content of peptidoglycan cross bridges in Staphylococcus capitis and Staphylococcus aureus. J Bacteriol 179: 4311-4318.
29. Takahashi, J., Komatsuzawa, H., Yamada, S., Nishida, T., Labischinski, H., Fujiwara, T., et al. (2002) Molecular characterization of an atl null mutant of Staphylococcus aureus. Microbiol Immunol 46: 601-612.
30. Thompson, J.S., and Shockman, J.D. (1969) A modification of the Park and Johnson reducing sugar determination suitable for the assay of insoluble materials: its application to bacterial cell wall. Anal Biochem 22: 260-268.
31. Ward, J.B., and Williamson, R. (1984) Bacterial autolysins: specificity and function. In Microbial Cell Wall Synthesis andAutolysis. Nombela, C. (ed.). Amsterdam: Elsevier Science Publishers B. V., pp. 159-166.
32. Yamada, S., Sugai, M., Komatsuawa, H., Nakashima, S., Oshida, T., Matsumoto, A., and Suginaka, H. (1996) An autolysin ring associated with cell separation of Staphylococcus aureus. J Bacteriol 178: 1565-1571.
33. Yothrer, J., Leopold, K., White, J., and Fischer, W. (1998) Generation and properties of a Streptococcus pneumoniae mutant which does not require choline or analogs for growth. J Bacteriol 180: 2093-2101.