A method for extracting genomic DNA from individual elaphostrongyline (Nematoda: Protostrongylidae) larvae and differentiation of Elaphostrongylus spp. from Parelaphostrongylus spp. by PCR assay

Journal of Veterinary Diagnostic Investigation

Volumn 17, Issue 6, 2005, Pages 585-588

  Chilton, Neil B ^a   Huby Chilton, Florence ^b   Lankester, Murray W ^b   Gajadhar, Alvin A ^b  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b CANADIAN FOOD INSPECTION AGENCY   (Canada)

Author keywords

Diagnosis; DNA extraction; Elaphostrongylus; Parelaphostrongylus; PCR

Indexed keywords

ELAPHOSTRONGYLUS; NEMATODA; PARELAPHOSTRONGYLUS; PROTOSTRONGYLIDAE;

EID: 27944461425     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870501700612     Document Type: Article

References (16)

1. Ball MC, Lankester MW, Mahoney SP: 2001, Factors affecting the distribution and transmission of Elaphostrongylus rangiferi (Protostrongylidae) in caribou (Rangifer tarandus caribou) of Newfoundland, Canada. Can J Zool 79:1265-1277.
2. Carreno RA, Hoberg EP: 1999, Evolutionary relationships among the Protostrongylidae (Nematoda: Metastrongyloidea) as inferred from morphological characters, with consideration of parasite-host evolution. J Parasitol 85:638-648.
3. Carreno RA, Lankester MW: 1993, Additional information on the morphology of the Elaphostrongylinae (Nematoda: Protostrongylidae) of North American Cervidae. Can J Zool 71:592-600.
4. Forrester DJ, Lankester MW: 1997, Extracting protostrongylid nematode larvae from ungulate feces. J Wild Dis 33:511-516.
5. Gajadhar A, Steeves-Gurnsey T, Kendall J, et al.: 2000, Differentiation of dorsal-spined elaphostrongyline larvae by polymerase chain reaction amplification of ITS-2 of rDNA. J Wild Dis 36:713-722.
6. Gajadhar AA, Tessaro SV: 1995, Susceptibility of mule deer (Odocoileus hemionus) and two species of North American molluscs to Elaphostrongylus cervi (Metastrongyloidea: Protostrongylidae). J Parasitol 81:593-596.
7. Gajadhar AA, Tessaro SV, Yates WDG: 1994, Diagnosis of Elaphostrongylus cervi infection in New Zealand red deer (Cervus elaphus) quarantined in Canada, and experimental determination of a new extended prepatent period. Can Vet J 35:433-437.
8. Gasser RB, Chilton NB, Hoste H, Beveridge I: 1993, Rapid sequencing of rDNA from single worms and eggs of parasitic helminths. Nucleic Acids Res 21:2525-2526.
9. Gray JB, Samuel WM, Shostak AW, Pybus MJ: 1985, Varestrongylus alpenae (Nematoda: Metastrongyloidea) in whitetailed deer (Odocoileus virginianus) of Saskatchewan. Can J Zool 63:1449-1454.
10. Handeland K, Sparboe O: 1991, Cerebrospinal elaphostrongylosis in dairy goats in northern Norway. J Vet Med B 38:755-761.
11. Huby-Chilton F, Chilton NB, Lankester MW, Gajadar AA: 2005, Single-strand conformation polymorphism (SSCP) analysis as a new diagnotic tool to distinguish dorsal-spined larvae or the Elaphostrongylinae (Nematoda: Protostrongylidae) from cervids. Vet Parasitol in press.
12. Kutz SJ, Hoberg EP, Policy L: 2001, Umingmakstrongylus pallikuukensis (Nematoda: Protostrongylidae) in gastropods: larval morphology, morphometrics, and development rates. J Parasitol 87:527-535.
13. Lankester MW: 2001, Extrapulmonary lungworms of cervids. In: Parasitic diseases of wild animals, eds. Samuel WM, Pybus MJ, Kocan AA, pp. 228-278. Mason Publishing Ltd, London.
14. Lankester MW, Fong D: 1989, Distribution of elaphostrongyline nematodes (Metastrongyloidea: Protostrongylidae) in Cervidae and possible effects of moving Rangifer spp. into and within North America. Alces 25:133-145.
15. Lankester MW, Fong D: 1998, Protostrongylid nematodes from caribou (Rangifer tarandus caribou) and moose (Alces alces) in Newfoundland. Rangifer 10(special issue):73-83.
16. Pybus MJ, Foreyt WJ, Crichton V: 1989, Identification of dorsal-spined larvae from free ranging wapiti (Cervus elaphus) in southwestern Manitoba, Canada. J Wild Dis 25:292-293.