Proteomic analysis of protein components in periodontal ligament fibroblasts

Journal of Periodontology

Volumn 76, Issue 10, 2005, Pages 1645-1653

  Reichenberg, E ^a   Redlich, M ^a   Cancemi, P ^b   Zaks, B ^a   Pitaru, S ^c   Fontana, S ^b   Pucci Minafra, I ^b   Palmon, A ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b UNIVERSITY OF PALERMO   (Italy)

^c TEL AVIV UNIVERSITY   (Israel)

Author keywords

Fibroblasts; Periodontal ligament; Proteome; Proteomics

Indexed keywords

ACTIN; CELL PROTEIN; CYTOSKELETON PROTEIN; PROTEOME; SCAFFOLD PROTEIN; TUBULIN; VIMENTIN;

ARTICLE; CELL CULTURE; CELL CYCLE; CELL FUNCTION; CELL MEMBRANE; CELL MOTILITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DETOXIFICATION; FIBROBLAST; GENETIC TRANSDUCTION; HOMEOSTASIS; HUMAN; HUMAN CELL; MASS SPECTROMETRY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PERIODONTAL LIGAMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN DATABASE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEOMICS; RNA TRANSLATION; STRESS; UNINDEXED SEQUENCE;

ADOLESCENT; CELLS, CULTURED; CHILD; CYTOSKELETAL PROTEINS; DATABASES, PROTEIN; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FEMALE; FIBROBLASTS; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; ISOELECTRIC FOCUSING; MALE; PEPTIDE MAPPING; PERIODONTAL LIGAMENT; PROTEINS; PROTEOME; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 27844597781     PISSN: 00223492     EISSN: None     Source Type: Journal    
DOI: 10.1902/jop.2005.76.10.1645     Document Type: Article

References (24)

1. Berkovitz BKB. The structure of the periodontal ligament: An update. Eur J Orthod 1990;12:51-76.
2. Sodek J, Limeback HF. Comparison of the rates of synthesis, conversion and maturation of type I and type III collagens in rat periodontal tissues. J Biol Chem 1979;254:10496-10502.
3. Shimono M, Ishikawa T, Ishikawa H, et al. Regulatory mechanisms of periodontal regeneration. Microsc Res Tech 2003;60:491-502.
4. Pitaru S, McCulloch CA, Narayanan SA. Cellular origins and differentiation control mechanisms during periodontal development and wound healing. J Periodontal Res 1994;29:81-94.
5. Lekic PC, Pender N, McCulloch CA. Is fibroblast heterogeneity relevant to the health, diseases, and treatments of periodontal tissues? Crit Rev Oral Biol Med 1997;8:253-268.
6. Han X, Amar S. Identification of genes differentially expressed in cultured human periodontal ligament fibroblasts vs. human gingival fibroblasts by DNA microarray analysis. J Dent Res 2002;81:399-405.
7. Kimoto S, Matsuzawa M, Matsubara S, et al. Cytokine secretion of periodontal ligament fibroblasts derived from human deciduous teeth: Effect of mechanical stress on the secretion of transforming growth factor-beta 1 and macrophage colony stimulating factor. J Periodontal Res 1999;34:235-243.
8. Palaiologou AA, Yukna RA, Moses R, Lallier TE. Gingival, dermal, and periodontal ligament fibroblasts express different extracellular matrix receptors. J Periodontol 2001;72:798-807.
9. Chaussain Miller C, Septier D, Bonnefoix M, et al. Human dermal and gingival fibroblasts in a three-dimensional culture: A comparative study on matrix remodeling. Clin Oral Investig 2002;6:39-50.
10. Seliger B, Kellner R. Design of proteome-based studies in combination with serology for the identification of biomarkers and novel targets. Proteomics 2002;2: 1641-1651.
11. Honore B. Genome- and proteome-based technologies: Status and applications in the postgenomic era. Expert Rev Mol Diagn 2001;1:265-274.
12. Palmon A, Roos H, Edel J, et al. Inverse dose-and time-dependent effect of basic fibroblast growth factor on the gene expression of collagen type I and matrix metalloproteinase-1 by periodontal ligament cells in culture. J Periodontol 2000;71:974-980.
13. Redlich M, Roos H, Reichenberg E, et al. The effect of centrifugal force on mRNA levels of collagenase, collagen type-1, tissue inhibitors of metalloproteinases and beta-actin in cultured human periodontal ligament fibroblasts. J Periodontal Res 2004;39:27-32.
14. Pucci-Minafra I, Fontana S, Cancemi P, Alaimo G, Minafra S. Proteomic patterns of cultured breast cancer cells and epithelial mammary cells. Ann N Y Acad Sci 2002;963:122-139.
15. Gorg A, Postel W, Gunther S. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 1988;9:531-546.
16. Bjellqvist B, Pasquali C, Ravier F, Sanchez JC, Hochstrasser D. A nonlinear wide-range immobilized pH gradient for two-dimensional electrophoresis and its definition in a relevant pH scale. Eledrophoresis 1993;14:1357-1365.
17. Hochstrasser DF, Harrington MG, Hochstrasser AC, Miller MJ, Merril CR. Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal Biochem 1988;173:424-435.
18. Swiss Institute of Bioinformatics. ExPASy proteomics server. Available at: http://www.expasy.org/. Accessed February 10, 2005.
19. Boraldi F, Bini L, Liberatori S, et al. Normal human dermal fibroblasts: Proteomic analysis of cell layer and culture medium. Electrophoresis 2003;24:1292-1310.
20. Swiss Institute of Bioinformatics. ExPASy proteomics server. WORLD-2DPAGE: Index to 2-D PAGE databases and services. Available at: http://www.expasy.org/ch2d/2d-index.html/. Accessed February 10, 2005.
21. Canadian Bioinformatics Resource. ExPASy Compute pI/MW tool. Available at: http://expasy.cbr.nrc.ca/tools/pi_tool.html/. Accessed February 10, 2005.
22. National Center for Biotechnology Information. Basic local alignment search tool. Available at: http://www.ncbi.nlm.nih.gov/BLAST/. Accessed February 10, 2005.
23. Phipps RP, Borrello MA, Blieden TM. Fibroblasts heterogeneity in the periodontium and other tissues. J Periodontal Res 1997;32:159-165.
24. Nollen EA, Morimoto RI. Chaperoning signaling pathways: Molecular chaperones as stress-sensing 'heat shock' proteins. J Cell Sci 2002;115:2809-2816.