Establishment and characterization of primary and subsequent subcultures of normal mouse urothelial cells

Folia Biologica

Volumn 51, Issue 5, 2005, Pages 126-132

  Kreft, Mateja Erdani ^a   Hudoklin, S ^a   Sterle, M ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Cell culture; Differentiation; Morphology; Mouse urothelium; Urinary bladder

Indexed keywords

UROPLAKIN; UVOMORULIN; VIMENTIN;

ANIMAL CELL; ANTIBODY LABELING; ARTICLE; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; CELL LABELING; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; FIBROBLAST; IMMUNOFLUORESCENCE; MOUSE; NONHUMAN; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; UROTHELIUM;

ANIMALIA;

EID: 27844559632     PISSN: 00155500     EISSN: 25337602     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Acharya, P., Beckel, J., Ruiz, W.G., Wang, E., Rojas, R., Birder, L., Apodaca, G. (2004) Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium. Am. J. Physiol. Renal Physiol. 287, F305-F318.
2. Bryant, P. L., Reid, L. M., Diala, E. S., Abbott, B. D., Buckalew, A. R. (2001) An in vitro model for murine ureteric epithelial cells. In Vitro Cell Dev. Biol. Anim. 37, 395-398.
3. deBoer, W. I., Rebel, J. M., Vermey, M., deJong, A. A., van der Kwast T. H. (1994) Characterization of distinct functions for growth factors in murine transitional epithelial cells in primary organotypic culture. Exp. Cell Res. 214, 510-518.
4. Ehmann, U. K., Terris, M. K. (2002a) Juxtacrine stimulation of normal and malignant human bladder epithelial cell proliferation. J. Urol. 167, 735-741.
5. Ehmann, U. K., Terris M. K. (2002b) Long-term culture of porcine bladder epithelial cells. In Vitro Cell Dev. Biol. Anim. 38, 137-141.
6. Guhe, C., Föllmann, W. (1994) Growth and characterization of porcine urinary bladder epithelial cells in vitro. Am. J. Physiol. 266, F298-F308.
7. Hicks, R. M. (1975) The mammalian urinary bladder: an accommodating organ. Biol. Rev. 50, 215-246.
8. Howlett, A. R., Hodges, G. M., Rowlatt, C. (1986) Epithelial-stromal interactions in the adult bladder: urothelial growth, differentiation, and maturation on culture facsimiles of bladder stroma. Dev. Biol. 118, 403-415.
9. Hu, P., Meyers, S., Liang, F.-X., Deng, F.-M., Kachar, B., Zeidel, M. L., Sun, T.-T. (2002) Role of membrane proteins in permeability barrier function: uroplakin ablation elevates urothelial permeability. Am. J. Physiol. Renal Physiol. 283, F1200-F1207.
10. Kreft, M. E., Sterle, M. (2000) The effect of lamina propria on the growth and differentiation of urothelial cells in vitro. Pflugers Arch. 440, R181-R182.
11. Kreft, M. E., Romih, R., Sterle, M. (2002) Antigenic and ultrastructural markers associated with urothelial cytodifferentiation in primary explant outgrowths of mouse bladder. Cell Biol. Int. 26, 63-74.
12. Kreft, M. E., Sterle, M., Veranič, P., Jezernik, K. (2005) Urothelial injuries and the early wound healing response: Tight junctions and urothelial cytodifferentiation. Histochem. Cell Biol. 123, 529-539.
13. Kurzrock, E. A., Lieu, D. K., DeGraffenried, L. A., Isseroff, R. R. (2005) Rat urothelium: improved techiques for serial cultivation, expansion, freezing and reconstitution onto acellular matrix. J. Urol. 173, 281-285.
14. Master, V. A, Wei, G., Liu, W., Baskin, L. A. (2003) Urothelium facilitates the recruitment and trans-differentiation of fibroblasts into smooth muscle in acellular matrix. J. Urol. 170, 1628-1632.
15. Scriven, S. D., Booth, C., Thomas, D. F. M., Trejdosiewicz, L. K., Southgate, J. (1997) Reconstitution of human urothelium from monolayer cultures. J. Urol. 158, 1147-1152.
16. Smith, B. A., Kennedy, W. J., Harnden, P., Selby, P. J., Trejdosiewicz, L. K., Southgate, J. (2001) Identification of genes involved in human urothelial cell-matrix interactions: implications for the progression pathways of malignant urothelium. Cancer Res. 61, 1678-1685.
17. Southgate, J., Hutton, K. A. R., Thomas, D. F. M., Trejdosiewicz, L. K. (1994) Normal human urothelial cells in vitro: proliferation and induction of stratification. Lab. Invest. 71, 583-594.
18. Sterle, M., Kreft, M. E., Batista, U. (1997) The effect of epidermal growth factor and transforming growth factor β1 on proliferation and differentiation of urothelial cells in urinary bladder explant culture. Biol. Cell 89, 29-41.
19. Sugasi, S., Lesbros, Y., Bisson, I., Zhang, Y. Y., Kucera, P., Frey P. (2000) In vitro engineering of human stratified urothelium: Analysis of its morphology and function. J. Urol. 164, 951-957.
20. Truschel, S. T., Ruitz, W. G., Shulman, T., Pilewski, J., Sun T.-T., Zeidel, M. L., Apodaca, G. (1999) Primary uroepithelial cultures. A model system to analyze umbrella cell barrier function. J. Biol. Chem. 274, 15020-15029.
21. Wu, X.-R, Manabe, M., Yu, J., Sun, T.-T. (1990) Large scale purification and immunolocalization of bovine uroplakins I, II and III. Molecular markers of urothelial differentiation. J. Biol. Chem. 256, 19170-19179.
22. Yu, J., Lin, J. H., Wu, X.-R., Sun, T.-T. (1994) Uroplakins Ia and Ib, two major differentiation products of bladder epithelium, belong to a family of four transmembrane domain (4TM) proteins. J. Cell. Biol. 125, 171-182.
23. Zhang, Y. Y., Ludwikowski, B., Hurst, R., Frey, P. (2001) Expansion and long-term culture of differentiated normal rat urothelial cells in vitro. In Vitro Cell Dev. Biol. Anim. 37, 419-429.