Molecular cloning of a novel rat gene Tsarg1, a member of the DnaJ/HSP40 protein family

DNA Sequence - Journal of DNA Sequencing and Mapping

Volumn 16, Issue 3, 2005, Pages 166-172

  Yang, Hong Mei ^a   Liu, Gang ^a   Nie, Zhao Yan ^a   Nie, Dong Song ^a   Deng, Yun ^a   Lu, Guang Xiu ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Azoospermia; Gene cloning; Rat HSP40; Testis apoptosis

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; HEAT SHOCK PROTEIN 40; HYBRID PROTEIN; PROTEIN DNAJ; TSARG1 PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELLULAR DISTRIBUTION; CHROMOSOME 1Q; CONTROLLED STUDY; DNA SEQUENCE; GENE IDENTIFICATION; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPERMATOCYTE; TESTIS; TISSUE DISTRIBUTION;

AMINO ACID SEQUENCE; ANIMALS; APOPTOSIS; BASE SEQUENCE; BLOTTING, NORTHERN; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; COS CELLS; DNA PRIMERS; DNA, COMPLEMENTARY; ESCHERICHIA COLI; EXONS; EXPRESSED SEQUENCE TAGS; FEMALE; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEINS; HEAT-SHOCK PROTEINS; HSP40 HEAT-SHOCK PROTEINS; INTRONS; MALE; MICE; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; OLIGOSPERMIA; OPEN READING FRAMES; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; RATS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, AMINO ACID; SPERMATOGENESIS; TESTIS; TISSUE DISTRIBUTION; TRANSFECTION;

ANIMALIA;

EID: 27844550504     PISSN: 10425179     EISSN: 10292365     Source Type: Journal    
DOI: 10.1080/10425170500129736     Document Type: Article

References (29)

1. Baccetti B, Collodel G, Piomboni P. 1996. Apoptosis in human ejaculated sperm cells (notulae seminologicae 9). J Submicrosc Cytol Pathol 28:587-596.
2. Bukau B, Horwich A. 1998. The Hsp70 and Hsp60 chaperone machines. Cell 92:351-366.
3. Cheetham ME, Caplan AJ. 1998. Structure, function and evolution of DnaJ: Conservation and adaptation of chaperone function. Cell Stress Chaperon 3:28-36.
4. Chen CH, Lee SS, Chen DC, Chien HH, Chen IC, Chu YN, Liu JY, Chen WH, Wu GJ. 2004. Apoptosis and kinematics of ejaculated spermatozoa in patients with varicocele. J Androl 25:348-353.
5. Douglas M Cyr, Langer T, Michael G. 1994. DnaJ-like proteins molecular chaperones and specific regulators of Hsp 70. Trend Biochem Sci 19:176-181.
6. Fu JJ, Li LY, Lu GX. 2002. Relationship between microdeletion on Y chromosome and patients with idiopathic azoospermia and severe oligozoospermia in the Chinese. Chinese Med J 115:72-75.
7. Gabai VL, Yaglom JA, Volloch V, Meriin AB, Force T, Koutroumanis M, et al. 2000. Hsp72-mediated suppression of c-Jun N-terminal kinase is implicated in development of tolerance to caspase-independent cell death. Mol Cell Biol 20:6826-6836.
8. Hunter ES, Dix DJ. 2001. Heat shock proteins Hsp70-1 and Hsp70-3 are necessary and sufficient to prevent arsenite-induced dysmorphology in mouse embryos. Mol Reprod Dev 59:285-293.
9. Jiang H, Li LY, Lu GX. 2001. Molecular cloning of genes related to apoptosis in spermatogenic cells of mouse. Acta Biochim Biophys Sin 33:421-425.
10. Li D, Lu GX. 2004. Identification and expression of a novel human testis-specific gene by digital differential display. Chinese Med J 117:1791-1796.
11. Liu G, Lu GX, Fu JJ, Liu SF, Xing XW. 2003. Molecular cloning of mTSARG3 gene related to apoptosis in mouse spermatogenic cells. Acta Biochim Biophys Sin 35:1133-1139.
12. Liu G, Lu GX, Xing XW. 2004. Molecular cloning of TSARG6 gene related to apoptosis in human spermatogenic cells. Acta Biochim Biophys Sin 36:93-98.
13. Lu Z, Cyr DM. 1998. The conserved carboxyl terminus and zinc finger-like domain of the co-chaperone Ydj1 assist Hsp70 in protein folding. J Biol Chem 273:5970-5978.
14. Mieusset R, Bujan L, Mondinat C, Mansat A, Pontonnier F, Grandjean H. 1987. Association of scrotal hyperthermia with impaired spermatogenesis in infertile men. Fertil Steril 48:1006-1011.
15. Mieusset R, Bujan L, Plantavid M, Grandjean H. 1989. Increased levels of serum follicle-stimulating hormone and luteinizing hormone associated with intrinsic testicular hyperthermia in oligospermic infertile men. J Clin Endocrinol Metab 68:419-425.
16. Nielsen H, Engelbrecht J, Brunak S, Heijne G. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10:1-6.
17. Ohtsuka K, Hata M. 2000. Molecular chaperone function of mammalian Hsp70 and Hsp40. Int J Hyperther 16:231-245.
18. Rassow J, von A O, Bömer U, Pfanner N. 1997. Molecular chaperones: Towards a characterization of the heat-shock protein 70 family. Trends Cell Biol 7:129-133.
19. Robinson D, Rock J, Menkin MR. 1968. Control of human spermatogenesis by induced changes of intrascrotal temperature. J Am Med Assoc 204:290-297.
20. Rockett JC, Mapp FL, Garges JB, Luft JC, Mori C, Dix DJ. 2001. Effects of hyperthermia on spermatogenesis, apoptosis, gene expression, and fertility in adult male mice. Biol Reprod 65:229-239.
21. Sarge KD, Cullen KE. 1997. Regulation of hsp expression during rodent Spermatogenesis. Cell Mol Life Sci 3:191-197.
22. Szabo A, Korszun R, Hartl FU, Flanagan J. 1996. A zinc finger-like domain of the molecular chaperone DnaJ is involved in binding to denatured protein substrates. EMBO J 15:408-417.
23. Wall D, Zylicz M, Georgopoulos C. 1995. The conserved G/F motif of the DnaJ chaperone is necessary for the activation of the substrate binding properties of the DnaK chaperone. J Biol Chem 270:2139-2144.
24. Wu Y, Li J, Jin Z, Fu Z, Sha B. 2005. The crystal structure of the c-terminal fragment of yeast Hsp40 Ydj1 reveals novel dimerization motif for Hsp40. J Mol Biol 346:1005-1011.
25. Xu J, Xu ZL, Jiang YT, Qian XJ, Huang YL. 1991. Cryptorchidism induces testicular germ cell apoptosis in mice testes and changes of Bcl-2, Bax protein expression. ACTA Anat Sin 30:82-85.
26. Yamamoto CM, Hikim AP, Lue Y, Portugal AM, Guo TB, Hsu SY, Salameh WA, Wang C, Hsueh AJ, Swerdloff RS. 2001. Impairment of spermatogenesis in transgenic mice with selective overexpression of Bcl-2 in the somatic cells of the testis. J Androl 22:981-991.
27. Yen P. 2001. The fragility of fertility. Nat Genet 29:243-244.
28. Zorgniotti AW, MacLeod J. 1973. Studies in temperature, human semen quality and varicocele. Fertil Steril 24:854-863.
29. Zorgniotti AW, Sealfon AI. 1988. Measurement of intrascrotal temperature in normal and subfertile men. J Reprod Fertil 82:563-566.